Electrochemical Determination of Vitamin D₃ in Pharmaceutical Products by Using Boron Doped Diamond Electrode

Abstract

A sensitive square‐wave voltammetry method was developed to determine cholecalciferol (vitamin D3) in pharmaceutical products at boron‐doped diamond electrode as a working electrode. Vitamin D3 provided a well‐defined voltammetric peak at around +1.00 V (vs. Ag/AgCl, 3.5 mol dm−3) in 0.02 mol dm−3 Britton‐Robinson buffer pH 5.0 prepared in 50 % ethanol. The influence of various factors such as type and pH of the supporting electrolyte, scan rate and square‐wave parameters were studied and optimized. Under optimum conditions, the oxidation peak current increased linearly with the concentration of vitamin D3 over the range of 2 to 200 μmol dm−3. The calculated limit of detection and limit of quantitation were 0.17 μmol dm−3 and 0.51 μmol dm−3, respectively. The boron‐doped diamond electrode exhibited specific recognition capability for cholecalciferol amongst possible interferences, and the determination of vitamin D3 was possible in samples such as commercial pharmaceutical products without complicated sample pretreatments.

Recent Point of Care (PoC) Electrochemical Testing Trends of New Diagnostics Platforms for Vitamin D

“…Vitamin D 3 could be detected in samples like commercial pharmaceutical products without the need for laborious sample pretreatment because the boron-doped diamond electrode demonstrated a specific recognition ability for cholecalciferol among potential interferences. [81] Fathima et al researched carbon nanotubes' electrocatalytic activity on Vitamin D 3 . The electro-oxidation of vitamin D 3 was found to be unaffected by carbonaceous and metallic impurities.…”

The development of wearable technologies and their potential for measuring nutrient intake: Towards precision nutrition

“…Similarly to water‐soluble vitamins, fat‐soluble vitamins can carry out electron transfer in organic solvents, making electrochemical assay a commonly used method for fat‐soluble vitamins detection. For example, a boron‐doped diamond electrode was developed to detect vitamin D 3 in pharmaceutical products using a square‐wave voltammetry method (Đurović et al, 2019). Samples need to be pre‐treated with organic solvents and reagents to separate vitamins and their carriers in order to improve sensing performance.…”

High-Performance, Nitrogen-Doped, Carbon-Nanotube-Based Electrochemical Sensor For Vitamin D3 Detection

“…In the present work, we develop an antibody-free nitrogen-doped CNT (NCNT)-based electrochemical sensor for vitamin D3 sensing in picomolar levels. The sensor has a much higher selectivity and lower LOD in comparison to many other carbon-based sensors used for vitamin D detection. − NCNT is successfully characterized by XRD, SEM, TEM, XPS, BET, EIS, and CV measurement techniques. The results show the superior electrochemical performance of NCNT and its sensitivity for detection of vitamin D3 as compared to previously used traditional techniques or sensors.…”