Electrochemical detection of urinary microRNAs via sulfonamide-bound antisense hybridisation

Smith, Daniel; Newbury, Lucy J.; Drago, Guido A.; Bowen, Timothy; Redman, James E.

doi:10.1016/j.snb.2017.06.069

Cited by 51 publications

(34 citation statements)

References 32 publications

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“…We are still beginning to understand the role of non-coding RNAs. Currently, the techniques used for their isolation and analyses (including Northern blotting, RT-qPCR, microarray, surface plasmon resonance and fluorescence-based techniques [ 103 ]), despite being technically advanced, do not provide results rapidly. Due to the fact that RNA-based sequences are quite small, their analysis frequently requires the conversion of transcripts into a pool of cDNAs constituting the sequencing library.…”

Section: Mirna Ncrna Lncrna and Lincrna Biomarkersmentioning

confidence: 99%

“…However, we believe that in several years, the results of the most significant findings will be translated into diagnostic tests, perhaps in manner less simple than performing dipstick tests, but nonetheless less complicated and time- and effort-consuming than present methods. Smith et al [ 103 ] made a step in this direction designing a method of electrochemical detection of miRNAs in a urine sample which requires minimal liquid handling in a straightforward dipstick-style test. Moreover, in their method urine sample can be small and there is no need for extensive miRNA extraction procedures as well as toxic and expensive reagents.…”

Section: Mirna Ncrna Lncrna and Lincrna Biomarkersmentioning

confidence: 99%

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Novel Biomarkers in the Diagnosis of Chronic Kidney Disease and the Prediction of Its Outcome

Rysz

Gluba-Brzózka²,

Franczyk

et al. 2017

IJMS

210

154

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In its early stages, symptoms of chronic kidney disease (CKD) are usually not apparent. Significant reduction of the kidney function is the first obvious sign of disease. If diagnosed early (stages 1 to 3), the progression of CKD can be altered and complications reduced. In stages 4 and 5 extensive kidney damage is observed, which usually results in end-stage renal failure. Currently, the diagnosis of CKD is made usually on the levels of blood urea and serum creatinine (sCr), however, sCr has been shown to be lacking high predictive value. Due to the development of genomics, epigenetics, transcriptomics, proteomics, and metabolomics, the introduction of novel techniques will allow for the identification of novel biomarkers in renal diseases. This review presents some new possible biomarkers in the diagnosis of CKD and in the prediction of outcome, including asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA), uromodulin, kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), miRNA, ncRNA, and lincRNA biomarkers and proteomic and metabolomic biomarkers. Complicated pathomechanisms of CKD development and progression require not a single marker but their combination in order to mirror all types of alterations occurring in the course of this disease. It seems that in the not so distant future, conventional markers may be exchanged for new ones, however, confirmation of their efficacy, sensitivity and specificity as well as the reduction of analysis costs are required.

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Section: Mirna Ncrna Lncrna and Lincrna Biomarkersmentioning

confidence: 99%

Section: Mirna Ncrna Lncrna and Lincrna Biomarkersmentioning

confidence: 99%

Novel Biomarkers in the Diagnosis of Chronic Kidney Disease and the Prediction of Its Outcome

Rysz

Gluba-Brzózka²,

Franczyk

et al. 2017

IJMS

210

154

View full text Add to dashboard Cite

show abstract

“…However, miRs appear to be ideal future biomarkers for urinary analysis because of their presence and stability in urine [ 19 ], their relatively easy detectability (by qRT-PCR), their stability even after freeze-thaw cycles, and their specificity to tissue or disease states. Therefore, it is noteworthy that Smith et al [ 74 ] reported a promising approach to detect urinary miRs with high sensitivity via sulfonamide-bound antisense hybridisation. The authors claim that this method has several significant advantages over circulating biomarker analysis including safety, cost, speed, and ease of conversion.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs as Urinary Biomarker for Oncocytoma

et al. 2018

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The identification of benign renal oncocytoma, its differentiation from malignant renal tumors, and their eosinophilic variants are a continuous challenge, influencing preoperative planning and being an unnecessary stress factor for patients. Regressive changes enhance the diagnostic dilemma, making evaluations by frozen sections or by immunohistology (on biopsies) unreliable. MicroRNAs (miRs) have been proposed as novel biomarkers to differentiate renal tumor subtypes. However, their value as a diagnostic biomarker of oncocytoma in urines based on mechanisms known in oncocytomas has not been exploited. We used urines from patients with renal tumors (oncocytoma, renal cell carcinoma: clear cell, papillary, chromophobe) and with other urogenital lesions. miRs were extracted and detected via qRT-PCR, the respective tumors analyzed by immunohistology. We found isocitrate dehydrogenase 2 upregulated in oncocytoma and oncocytic chromophobe carcinoma, indicating an increased Krebs cycle metabolism. Since we had shown that all renal tumors are stimulated by endothelin-1, we analyzed miRs preidentified by microarray after endothelin-1 stimulation of renal epithelial cells. Four miRs are proposed as presurgical urinary biomarkers due to their known regulatory mechanism in oncocytoma: miR-498 (formation of the oncocytoma-specific slice-form of vimentin, Vim3), miR-183 (associated with increased CO2 levels), miR-205, and miR-31 (signaling through downregulation of PKC epsilon, shown previously).

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“…Unspecific protein adsorption and matrix effects appear when using patient samples with label-free techniques. In an impedimetric hybridization-based biosensor for miRNA-21 reporting fM LOD, urine was digested with proteinase K and then filtrated (Smith et al, 2017). That way, an antifouling strategy to protect the surface is unnecessary while miRNA integrity is preserved, though direct detection is not viable.…”

Section: Dna-based Yellow Diagnosticsmentioning

confidence: 99%

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Miranda‐Castro

Palchetti

de‐los‐Santos‐Álvarez

2020

Front. Chem.

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Latest technological advancement has tremendously expanded the knowledge on the composition of body fluids and the cancer-associated changes, which has fueled the replacement of invasive biopsies with liquid biopsies by using appropriate specific receptors. DNA emerges as a versatile analytical reagent in electrochemical devices for hybridization-based or aptamer-based recognition of all kind of biomarkers. In this mini review, we briefly introduce the current affordable targets (tumor-derived nucleic acids, circulating tumor cells and exosomes) in body fluids, and then we provide an overview of selected electrochemical methods already applied in clinical samples by dividing them into three large categories according to sample type: red (blood), yellow (urine), and white (saliva and sweat) diagnostics. This review focuses on the hurdles of the complex matrices rather than a comprehensive and detailed revision of the format schemes of DNA-based electrochemical sensing. This diverse perspective compiles some challenges that are often forgotten and critically underlines real sample analysis or clinical validation assays. Finally, the needs and trends to reach the market are briefly outlined.

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Electrochemical detection of urinary microRNAs via sulfonamide-bound antisense hybridisation

Abstract: HighlightsA modified glassy carbon electrochemical sensor for microRNAs was developed.The electrode allowed detection of femtomolar concentrations of miR-21.The method was applied to detection of urinary miR-21.

Cited by 51 publications

References 32 publications

Novel Biomarkers in the Diagnosis of Chronic Kidney Disease and the Prediction of Its Outcome

Novel Biomarkers in the Diagnosis of Chronic Kidney Disease and the Prediction of Its Outcome

MicroRNAs as Urinary Biomarker for Oncocytoma

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

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