Electrochemical detection of the neomycin phosphotransferase gene (NPT-II) in transgenic plants with a novel DNA biosensor

Abstract

A novel DNA electrochemical biosensor is described for the detection of neomycin phosphotransferase gene (NPT-II), a selection marker for transgenic plants. A thiol-modified capture probe immobilized onto the surface of a gold electrode and a biotinylated signaling probe were designed to be complementary to target regions of NPT-II flanked by PCR primers to eliminate false-positive signals from non-specific PCR products. The electrochemical assay of hybrids on the electrode surface was evaluated by means of both cyclic voltammetry (CV) and square wave voltammetry (SWV) after the coupling of biotinylated catalase with streptavidin-modified hybrids based on dendritic signal magnification and subsequent formation of polymerized aniline (PAn). It has been revealed that the sensor showed a linear increase within the target concentration (1.0-100 9 10 -6 mmol L -1 ). The limit of detection was about 0.2 9 10 -6 mmol L -1 and the specificity was enhanced significantly.

Iron/iron oxide-based magneto-electrochemical sensors/biosensors for ensuring food safety: recent progress and challenges in environmental protection

“…110 Streptavidin-biotin, avidin-biotin, and fluorescein-anti-Fab-fluorescein compounds also have been used to bind enzyme to DNA sequences. Alkaline phosphatase, 111,112 catalase, 113 glucose oxidase and horseradish peroxidase 114 are the most commonly used enzymes. Recently, Liao and coworkers 114 have used a two-enzyme approach, which includes an enzymatic cascade reaction with two different enzymes.…”

“…Table 1 ref. 50–175 summarizes the types of food contamination studied using the electrochemical sensors based on iron magnetic beads.…”

3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize

“…These devices have been reported in the literature for the binding of the GMO capture strand onto a surface of carbon or gold electrodes [8][9][10][11], or complex nanostructures such as composites of grapheneTiO 2 nanorods [12] and carbon nanotubes [13]. The use of nanostructured materials has been intensely increased since these nanomaterials constitute new platforms for biomolecular sensing that provide improved sensitivity and amenability to miniaturization [14][15][16].…”