Electrochemical detection of short sequences of hepatitis C 3a virus using a peptide nucleic acid-assembled gold electrode

Hejazi, Mohammad Saeid; Pournaghi-Azar, M.H.; Ahour, F.

doi:10.1016/j.ab.2009.11.019

Cited by 59 publications

(32 citation statements)

References 52 publications

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“…Notably, higher temperatures, solutions with low ionic strength and shorter sequences can be employed. 29,30 Electrochemical DNA detection is facilitated by redox-active probes, which are introduced into the PNA recognition layer as free-diffusing redox mediator, 31 redox-active intercalators or minor-groove binders 28,[32][33][34][35] or as covalently bound redox labels. [36][37][38][39] Label-free PNA-based biosensors were, in general, exploited using electrochemical impedance spectroscopy, 31,40,41 Scanning…”

Section: Metal-containing Pnas For Biosensing Purposesmentioning

confidence: 99%

Metal-containing peptide nucleic acid conjugates

2011

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Peptide Nucleic Acids (PNAs) are non-natural DNA/RNA analogues with favourable physicochemical properties and promising applications. Discovered nearly 20 years ago, PNAs have recently re-gained quite a lot of attention. In this Perspective article, we discuss the latest advances on the preparation and utilisation of PNA monomers and oligomers containing metal complexes. These metalconjugates have found applications in various research fields such as in the sequence-specific detection of nucleic acids, in the hydrolysis of nucleic acids and peptides, as radioactive probes or as modulators of PNA˙DNA hybrid stability, and last but not least as probes for molecular and cell biology.

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Section: Metal-containing Pnas For Biosensing Purposesmentioning

confidence: 99%

Metal-containing peptide nucleic acid conjugates

2011

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“…A PNA probe was designed to recognize the target DNA related to tumour necrosis factor. Hejazi et al (2010) [216] developed an electrochemical DNA biosensor, using a gold electrode modified with a self-assembled monolayer composed of a PNA probe and 6-mercapto-1-hexanol. They covalently attached first the 14-mer PNA probe related to the hepatitis C virus genotype 3a (pHCV3a) core/E1 region on the electrode.…”

Section: Peptide Nucleic Acids (Pnas) Biosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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“…We did not find any report concerning the development of electrochemical biosensor for direct detection and discrimination of DNA sequence and single base mismatch in either double-stranded oligonucleotide (ds-oligonucleotide) chains or double-strand PCR products without denaturating dsDNA into ssDNA. We have previously reported the electrochemical detection of single-stranded DNA (ssDNA), using methylene blue (MB) [19][20][21] and brilliant cresyl blue (BCB) [22] reduction signals and also utilizing guanine oxidation signal [23][24][25]. Recently we have reported the development of a electrochemical biosensor for direct detection of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a [26].…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Electrochemical Biosensor for Detection and Discrimination of DNA Sequence and Single Base Mutation in dsDNA Samples Based on PNA‐dsDNA Hybridization – a new Platform Technology

et al. 2010

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In spite of the extensive attention paid on the development of various DNA detection strategies, very few studies have been reported regarding direct detection of DNA sequence and mutation in dsDNA. Here, we describe the feasibility of detection and discrimination of target DNA sequences and single base mutations (SBM) directly in double-stranded oligonucleotides and PCR products without the need for denaturation of the target dsDNA samples. This goal was achieved by employing a peptide nucleic acid (PNA) chain, self-assembled on the gold electrode as a probe, which binds to dsDNA and forms PNA-dsDNA hybrid.

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Electrochemical detection of short sequences of hepatitis C 3a virus using a peptide nucleic acid-assembled gold electrode

Cited by 59 publications

References 52 publications

Metal-containing peptide nucleic acid conjugates

Metal-containing peptide nucleic acid conjugates

Electrospinning-Based Nanobiosensors

Development of a Novel Electrochemical Biosensor for Detection and Discrimination of DNA Sequence and Single Base Mutation in dsDNA Samples Based on PNA‐dsDNA Hybridization – a new Platform Technology

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