Electrochemical Detection in Polymer Microchannels

Rossier, Joël S.; Roberts, Matthew; Ferrigno, Rosaria; Girault, Hubert H.

doi:10.1021/ac981382i

Cited by 140 publications

(140 citation statements)

References 60 publications

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…47,50,51 The width, depth and length of the prepared microchannels were 35 mm, 40 mm and 6.5 cm for the open microchannel, 15 mm, 20 mm and 4 cm for the working electrode (WE) and 35 mm, 40 mm and 2 cm for the CE/RE. The open microchannel and the one dedicated as WE were aligned at each side of the PET film, while the open microchannel and the one used as CE/RE were perpendicularly connected over the same PET face (Fig.…”

Section: Fountain Pen Probe Preparationmentioning

confidence: 99%

Fountain pen for scanning electrochemical microscopy

et al. 2010

View full text Add to dashboard Cite

A fountain pen probe has been developed to extend the scope of scanning electrochemical microscopy (SECM) experiments to dry surfaces. The fountain pen is fabricated by UV-photoablation of a polyethylene terephthalate (PET) film and consists on one side of one microchannel for flowing an electrolyte solution to the open tip, integrating a reference/counter electrode and on the other side a carbon track. The exposed tip of the track forms the working electrode located close to the microchannel outlet. The fountain pen can operate in a pointillist mode where a nanolitre droplet at the bottom of the probe connects it to a well-defined surface area to study locally the substrate, but can also operate in a scanning mode leaving a linear wet track of solution behind it to monitor the surface activity. The electrochemical characterization of the proposed fountain pen probe was performed by cyclic voltammetry, approach curves and lateral line scans over insulating and conductive substrates, showing that the flow rate and the probe-substrate distance have a major influence on its electrochemical behavior. An SECM image of a gold on glass EPFL logo is presented as a proof-ofconcept that fountain pen probes can be employed for the detection of surface activity when scanning in a contact regime.

show abstract

Section: Fountain Pen Probe Preparationmentioning

confidence: 99%

Fountain pen for scanning electrochemical microscopy

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Although most detection protocols for microfluidic devices use off-chip fluorescence, carbon electrodes can be integrated into microfluidic devices for electrochemical detection (29,30). The electrodes are constructed from carbon ink containing a polymer binder.…”

Section: Applicationsmentioning

confidence: 99%

Peer Reviewed: Polymeric Microelectromechanical Systems.

Soper¹,

et al. 2000

View full text Add to dashboard Cite

show abstract

“…For example, analytical separations such as capillary electrophoresis (CE) [3,4], two-dimensional electrokinetic separations [5], liquid chromatography (LC) [6], reaction and detection microchambers [7][8][9] have been implemented in the microfluidic format. More recently, analytical microfluidic systems have caught interest in the particular field of proteomics [10,11] because of the high analytical constraints demanded by this field, namely the low amount of sample usually available, the high complexity of the initial protein mixture, and the need for high-throughput analysis [12].…”

mentioning

confidence: 99%

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting. (J Am Soc Mass Spectrom 2004, 15, 1767-1779

show abstract

Electrochemical Detection in Polymer Microchannels

Cited by 140 publications

References 60 publications

Fountain pen for scanning electrochemical microscopy

Fountain pen for scanning electrochemical microscopy

Peer Reviewed: Polymeric Microelectromechanical Systems.

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Contact Info

Product

Resources

About