Electrochemical detection as an alternative to UV in RP‐HPLC peptide mapping

Abstract

This article describes the possibility of electrochemical (EC) detection as an alternative to ultraviolet (C!) detection doing peptide mapping. The high sensitivity and selectivity of the electrochemical detection can be applied to determine some digested peptides. This approach demonstrated that peptides containing electroactive amino acids can be determined by EC detection. Furthermore, upon irradiation, noneiectroactive amino acids and peptides can be made electroactive, thereby increasing the sensitivity and selectivity of peptide mapping. A variety of effects are discussed to help in understanding the possibility of this useful approach.

Resolution of Differential Pulse Voltammetric Peaks Using Genetic Algorithm Based Variable Selection-Partial Least Squares and Principal Component-Artificial Neural Networks

“…Numerous procedures have been described for the determination of amino acids in complex biological samples involving liquid chromatographic separations followed by detection. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] Because the majority of amino acids do not possess chromophoric moieties, their photometric detection requires pre-injection or post-column derivatization using appropriate chromophoric reagents. [1][2][3][4][5] Direct electrochemical detection without a prior derivatization is gaining prominence for amino acids in conjunction with flow injection analysis (FIA), high performance liquid chromatography (HPLC), and capillary electrophoresis (CE).…”

“…[1][2][3][4][5] Direct electrochemical detection without a prior derivatization is gaining prominence for amino acids in conjunction with flow injection analysis (FIA), high performance liquid chromatography (HPLC), and capillary electrophoresis (CE). [6][7][8][9][10][11][12] The noble electrodes Pt, Au and glassy carbon are well known to exhibit sensitive anodic response for small polar organic molecules including amino acids. However, the anodic response is quickly attenuated as a consequence of electrode fouling by adsorption of detected products and/or formation of inert surface oxides.…”

Theory of bulk and flow electrolysis and approach to parameter optimisation for chromatographic electrochemical detection

“…amines, phenols, mercaptans, aromatic nitro and halogen compounds, aldehydes and ketones) [2][3][4][5] to inorganic ones (anions and cations) [6][7]. Nowa d ays electro chemical detection is ex t e n s ive ly adopted in biochemistry for organic analytes (sugars, pept i d e s , c at e ch o l a m i n e s , v i t a m i n s , d rugs...) [8][9][10][11][12]; it is also e m p l oyed for env i ronmental analysis of organic contaminants (phenols, pesticides...) [13][14], whereas it is less commonly applied to metal ion determination [15][16].…”