Electrochemical Control of Growth Factor Presentation To Steer Neural Stem Cell Differentiation

Herland, Anna; Persson, Kristin; Lundin, Vanessa; Fahlman, Mats; Berggren, Magnus; Jager, Edwin; Teixeira, Ana I.

doi:10.1002/anie.201103728

Cited by 58 publications

(42 citation statements)

References 27 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, GAG's such as HA, CS, DS and heparin sulfate (HS) are often incorporated through doping into conducting polymers with the intention of promoting favourable interactions with proteins to improve biocompatibility 38,45 . For example, the interaction of heparin with the protein, fibroblast growth factor-2 (FGF-2), has been implicated in the ability to control stem cell differentiation on PEDOT/heparin films 47 .…”

Section: Resultsmentioning

confidence: 99%

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Gelmi

Higgins

Wallace

2013

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

The interaction of ECM proteins is critical in determining the performance of materials used in biomedical applications such as tissue regeneration, implantable bionics and biosensing. Methods: To improve our understanding of ECM protein-conducting polymer interactions, we have used Atomic Force Microscopy (AFM) to elucidate the interactions of fibronectin (FN) on polypyrrole (PPy) doped with different glycosaminoglycans. Results: We were able to classify four main types of FN interactions, including those related to 1) non-specific adhesion, 2) protein unfolding and subsequent unbinding from the surface, 3) desorption and 4) interactions with no adhesion. FN adhesion on PPy/hyaluronic acid showed a significantly lower density of surface adhesion with the adhesion restricted to nodule structures, as opposed to their peripheries, of the polymer morphology. In contrast, PPy/chondroitin sulfate showed a significantly higher density of surface adhesion to the point where the distribution of adhesion effectively masked the topography. Through conductive AFM imaging, we found that the conductive regions correlated with regions of FN adhesion. Conclusions: Given that the conductivity requires doping of the polymer, these findings suggest that FN adhesion is mediated by interactions with chondroitin sulfate and hyaluronic acid at the polymer surface and may be indicative of specific interactions due to contributions from electrostatic attraction between the FN and sulfate/anionic groups of the dopants. General significance: This study demonstrates the ability of AFM to resolve the protein-conducting polymer interactions at the molecular and nanoscale level, which will be important for interfacing these polymer materials with biological systems. This article is part of a Special Issue entitled Organic Bioelectronics -Novel Applications in Biomedicine.

show abstract

Section: Resultsmentioning

confidence: 99%

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Gelmi

Higgins

Wallace

2013

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

show abstract

“…They have been used for bio-sensing [5][6][7], as electrodes for stimulation or recording [8,9], for controlling cell adhesion, proliferation [10][11][12] and stem-cell differentiation [13], for controlled drug release [14,15] and even to mechanically stimulate cells [16]. They can be fabricated electrochemically using various counter ions [17,18] and modified with a variety of biomolecules [5,6,19].…”

Section: Introductionmentioning

confidence: 99%

Tunable conjugated polymers for bacterial differentiation

Golabi

Turner

Jager

2016

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

A novel rapid method for bacterial differentiation is explored based on the specific adhesion pattern of bacterial strains to tunable polymer surfaces. Different types of counter ions were used to electrochemically fabricate dissimilar polypyrrole (PPy) films with diverse physicochemical properties such as hydrophobicity, thickness and roughness. These were then modulated into three different oxidation states in each case. The dissimilar sets of conducting polymers were Principal Component Analysis showed that each strain of bacteria had its own specific adhesion pattern. Hence, they could be discriminated by this simple, label-free method. , based on tunable polymer arrays combined with pattern recognition.

show abstract

“…[41] Due to its biocompatibility [42] and excellent stability when compared to polypyrrole, [43] PEDOT, synthesised using different methods and with different counter ions, has been increasingly used within bioelectronics applications. Examples of such applications include drug delivery, [7] sensors, [44] interactions with cells [45,46] and as the active material in ionic transport devices (iontronics).…”

Section: Pedotmentioning

confidence: 99%

“…[63] As heparin also binds several growth factors, redox switching can thus be used to control the amount of growth factors available to cells. [45] …”

Section: Stimulation Through the Substratementioning

confidence: 99%

“…Surfactants can be added to circumvent the low monomer solubility in water, [160,167] but it is also possible to dissolve sufficient amounts of monomer with long stirring times. [45,168] The applied potential must be above the oxidation potential of the monomer but sufficiently low to avoid overoxidation, [169] which results in non-conducting films. The thickness will depend largely on the charge passed during polymerisation, but also on the counter ion.…”

Section: Electropolymerisationmentioning

confidence: 99%

See 1 more Smart Citation

Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces

Persson¹

2014

View full text Add to dashboard Cite

In the field of bioelectronics various electronic materials and devices are used in combination with biological systems in order to create novel applications within cell biology and medicine. A famous example of a successful bioelectronics application is the pacemaker. Metals are the most common electrical conductors, whereas polymers are generally considered being insulators. However, in the late 1970s it was shown that a special class of polymers with conjugated double bonds, could in fact, after some chemical modifications, conduct electricity. This was the start of the research field known as conducting polymers, and then later on organic electronics, a research area that has grown rapidly during the last decades. Conjugated polymers are also suitable to interact and interface with cells and tissues, as they are generally soft, flexible and biocompatible. In addition, their chemical properties can be tailor-made through synthesis to fit biological requirements and functions. During the last years applications using organic bioelectronics have become numerous. This thesis describes applications based on different conjugated polymers aiming to stimulate and control cell cultures. When culturing cells it is of interest to be able to control events such as adhesion, spreading, proliferation, differentiation and detachment. First, the impact of different polymer compositions and redox states on the adhesion of bacteria and subsequent biofilm formation was investigated. Similar polymer electrodes were also used to steer differentiation of neural stem cells, through changes in the surface exposure of a relevant biomolecule. Controlled delivery of molecules was achieved by coating nanoporous membranes with polymers that swell and contract when changing the redox state. Finally, electronic control over cell detachment using a water-soluble polymer was achieved. When applying a positive potential to this polymer, it swells, cracks and finally detaches, taking the cells that was cultured on top along with it. Together, the work and results presented in this thesis demonstrate a versatile conjugated polymer technology to achieve electronic control of the different growth stages of cell cultures as well as cellular functions. POPULÄRVETENSKAPLIG SAMMANFATTNINGOlika plaster finns numera överallt omkring oss och utgör ett av de absolut vanligaste materialen i vår vardag. Även elektronik har blivit en naturlig del av vår tillvaro och finns i en mängd olika produkter. Inom medicinsk teknik kommer allt fler applikationer som innefattar elektronik, ett exempel är pacemakern. Arbetet som ligger till grund för denna avhandling strävar efter att kombinera plaster och elektronik för tillämpningar inom medicin och cellbiologi.Plaster består till största delen av polymerer samt olika tillsatser för att få ett material med önskade egenskaper. Polymerer är i sin tur uppbyggda av långa kedjor av identiska molekylära byggstenar, så kallade monomerer. Monomerens kemi och struktur bestämmer även egenskaperna hos polymeren. Med hjä...

show abstract

Electrochemical Control of Growth Factor Presentation To Steer Neural Stem Cell Differentiation

Cited by 58 publications

References 27 publications

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Tunable conjugated polymers for bacterial differentiation

Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces

Contact Info

Product

Resources

About