Electrochemical biosensors for detection of avian influenza virus--current status and future trends.

Grabowska, Iwona; Malecka, Kamila; Jarocka, Urszula; Radecki, Jerzy; Radecka, Hanna

doi:10.18388/abp.2014_1866

Cited by 47 publications

(24 citation statements)

References 63 publications

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“…From electrochemical measurement methods, electrochemical impedance spectroscopy (EIS) is favorable thanks to real-time reaction monitoring, various data generation (about the electrode structure, and chemical and psychical changes during analyte binding), being label-free, and having low-current requirements and a nondestructive impact on the analyte [43].…”

Section: Introductionmentioning

confidence: 99%

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Cebula¹,

Żołędowska²,

Dziąbowska³

et al. 2019

Sensors

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The present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of Psl were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 103–1.2 × 105 CFU/mL (colony-forming unit) with an R2 coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.

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Section: Introductionmentioning

confidence: 99%

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Cebula¹,

Żołędowska²,

Dziąbowska³

et al. 2019

Sensors

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show abstract

“…Little success is also achieved with few potential molecules for novel therapeutic, antimicrobial, and drug delivery. Invention on this line leads to discovery of electrochemical biosensors as reliable analytical devices for pathogen detection of avian influenza virus in the complex matrices (Grabowska et al, 2014 ). More recent report revealed potential applications of affinity-based biosensors in sport medicine and doping control analysis (Mazzei et al, 2014 ).…”

Section: Current Research Trends Future Challenges and Limitations Omentioning

confidence: 99%

Recent Advances in Biosensor Technology for Potential Applications – An Overview

Vigneshvar

Sudhakumari

Senthilkumaran

et al. 2016

Front. Bioeng. Biotechnol.

440

237

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Imperative utilization of biosensors has acquired paramount importance in the field of drug discovery, biomedicine, food safety standards, defense, security, and environmental monitoring. This has led to the invention of precise and powerful analytical tools using biological sensing element as biosensor. Glucometers utilizing the strategy of electrochemical detection of oxygen or hydrogen peroxide using immobilized glucose oxidase electrode seeded the discovery of biosensors. Recent advances in biological techniques and instrumentation involving fluorescence tag to nanomaterials have increased the sensitive limit of biosensors. Use of aptamers or nucleotides, affibodies, peptide arrays, and molecule imprinted polymers provide tools to develop innovative biosensors over classical methods. Integrated approaches provided a better perspective for developing specific and sensitive biosensors with high regenerative potentials. Various biosensors ranging from nanomaterials, polymers to microbes have wider potential applications. It is quite important to integrate multifaceted approaches to design biosensors that have the potential for diverse usage. In light of this, this review provides an overview of different types of biosensors being used ranging from electrochemical, fluorescence tagged, nanomaterials, silica or quartz, and microbes for various biomedical and environmental applications with future outlook of biosensor technology.

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“…Lum et al (2015) had developed an impedance aptasensor which integrated a microfluidics flow cell and an interdigitated microelectrode for specific detection H5N1 virus with a detection limit of 0.0128 HAU (Lum et al, 2015). A more comprehensive review of biosensors for detection AIVs was reported by Grabowska et al (2014) and Wang and Li (2016) (Grabowska et al, 2014;Wang and Li, 2016). Biosensors for AIVs detection are not readily applied currently.…”

Section: Biosensormentioning

confidence: 99%

Detection methods of avian influenza - current and novel approaches

Yee¹,

Shamsuddin²,

Nizam³

et al. 2019

MJM

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Avian influenza (AI), caused by the avian strain of influenza A virus (AIV) is one of the significant health concerns globally. Human infections with AI viruses were reported sporadically and often exhibited high mortality and morbidity rate. AI outbreaks also influenced the safety of the food supply and caused significant economic losses. Immediate control measures are required during AI outbreaks in poultry to prevent further viruses spreading. Hence, accurate, sensitive, and rapid detection methods are pivotal for decision making. Traditional methods of detection, such as virus isolation in embryonated chicken eggs, immuno-based methods, and nucleic acid amplification method, pose different limitations. These always grab the attention of researchers to improve existing methods or invent novel diagnostic approaches to compensate for the shortcoming of current methods applied. However, the method of choice is highly dependent on the availability of facilities and resources. Among the detection methods, reverse transcription-polymerase chain reaction (RT-PCR) is the most favourable method used for detecting AIV. However, a constant review of the virus genome is crucial to maintain the assay's sensitivity. More comprehensive research and evaluation study are needed for new diagnostic approaches.

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Electrochemical biosensors for detection of avian influenza virus--current status and future trends.

Cited by 47 publications

References 63 publications

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Recent Advances in Biosensor Technology for Potential Applications – An Overview

Detection methods of avian influenza - current and novel approaches

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