Electrochemical biosensors based on in situ grown carbon nanotubes on gold microelectrode array fabricated on glass substrate for glucose determination

Abstract

A highly sensitive electrochemical sensor is reported for glucose detection using carbon nanotubes grown in situ at low temperatures on photolithographically defined gold microelectrode arrays printed on a glass substrate (CNTs/Au MEA). One of the main advantages of the present design is its potential to monitor 64 samples individually for the detection of glucose. The selectivity of the fabricated MEA towards glucose detection is achieved via modification of CNTs/Au MEA by immobilizing glucose oxidase (GOx) enzyme in the matrix of poly (paraphenylenediamine) (GOx/poly (p-PDA)/CNTs/Au MEA). The electrocatalytic and electrochemical responses of the proposed sensing platform towards glucose determination were examined via cyclic voltammetry and electrochemical impedance spectroscopy. The developed impedimetric biosensor exhibits a good linear response towards glucose detection, i.e., 0.2–27.5 µM concentration range with sensitivity and detection limits of 168.03 kΩ−1 M−1 and 0.2 ± 0.0014 μM, respectively. The proposed glucose biosensor shows excellent reproducibility, good anti-interference property, and was successfully tested in blood serum samples. Further, the applicability of the proposed sensor was successfully validated through HPLC. These results supported the viability of using such devices for the simultaneous detection of multiple electroactive biomolecules of physiological relevance. Graphical Abstract

Sensitivity enhancement of a Cu (II) metal organic framework-acetylene black-based electrochemical sensor for ultrasensitive detection of imatinib in clinical samples

“…In contrast, in the low-frequency region, the straight lines correspond to ion diffusion in redox reactions. In addition, the change in R ct value is determined by the characteristics of the modifiers and modification reaction on the surface ( Singh et al, 2023 ).…”

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“…Moreover, the developed biosensor was extended for the detection of fentanyl spiked in urine samples. In another study, a glucose biosensor was fabricated using in situ grown CNTs over a gold microelectrode printed on a glass substrate [ 69 ]. Finally, GOD was encapsulated by a polymer matrix made of paraphenylenediamine (p-PDA) over the CNT/AuE to obtain the desired biosensor.…”

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