Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform

Torati, Sri Ramulu; Reddy, Venu; Yoon, Seok Soo; Kim, CheolGi

doi:10.1016/j.bios.2015.11.098

Cited by 68 publications

(23 citation statements)

References 43 publications

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“…In addition to traditional PCR and hybridization based methods, there have been numerous reports of alternative technologies for ultrasensitive detection of nucleic acids (Lapitan et al 2015; Sun et al 2014). These have exploited innovative DNA nanotechnology schemes and a variety of readouts including electrochemical detection, quantum dots, and derivatized nanoparticles (Bernacka-Wojcik et al 2013; Hwang et al 2014; Torati et al 2016; Yan et al 2015). However, the clinical utility of these methods has not been established and few of these studies focus on drug resistance detection in Mtb.…”

Section: Discussionmentioning

confidence: 99%

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Bengtson

Homolka

Niemann

et al. 2017

Biosensors and Bioelectronics

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Current diagnostic tools for Mycobacterium tuberculosis (Mtb) have many disadvantages including low sensitivity, slow turnaround times, or high cost. Accurate, easy to use, and inexpensive point of care molecular diagnostic tests are urgently needed for the analysis of multidrug resistant (MDR) and extensively drug resistant (XDR) Mtb strains that emerge globally as a public health threat. In this study, we established proof-of-concept for a novel diagnostic platform (TB-DzT) for Mtb detection and the identification of drug resistant mutants using binary deoxyribozyme sensors (BiDz). TB-DzT combines a multiplex PCR with single nucleotide polymorphism (SNP) detection using highly selective BiDz sensors targeting loci associated with species typing and resistance to rifampin, isoniazid and fluoroquinolone antibiotics. Using the TB-DzT assay, we demonstrated accurate detection of Mtb and 5 mutations associated with resistance to three anti-TB drugs in clinical isolates. The assay also enables detection of a minority population of drug resistant Mtb, a clinically relevant scenario referred to as heteroresistance. Additionally, we show that TB-DzT can detect the presence of unknown mutations at target loci using combinatorial BiDz sensors. This diagnostic platform provides the foundation for the development of cost-effective, accurate and sensitive alternatives for molecular diagnostics of MDR- and XDR-TB.

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Section: Discussionmentioning

confidence: 99%

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Bengtson

Homolka

Niemann

et al. 2017

Biosensors and Bioelectronics

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“…Several mechanisms have been reported regarding the interaction between MB and ssDNA based on the electrochemical behavior [ 28 , 54 ]. Fundamentally, the most reported MB binding modes based on their binding to nucleic acid are electrostatic interactions between cationic MB and anionic DNA, intercalation of MB in the major or minor grooves of dsDNA helix and preferential binding between MB and guanine bases [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

“…To the best of our knowledge, there are not many reports [ 14 , 28 ] that study conducting polymer nanofiber-graphene composite as a biosensor for the detection Mycobacterium tuberculosis and their properties as modified electrodes. This work reports the behaviour of polyaniline/graphene (PANI/GP) composite nanofibers as a DNA biosensor.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis

Mohamad

Zaid

Abdullah

et al. 2017

Sensors

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This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of Mycobacterium tuberculosis by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10−6–10−9 M with the detection limit of 7.853 × 10−7 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors.

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“…Tuberculosis (TB) is one of the deadliest infectious diseases that became a significant public health problem worldwide [1]. The disease is mainly caused by the infection of Mycobacterium tuberculosis, which can be transmitted via minute aerosol droplets such as coughing, sneezing, or even talking by an infected TB person [2]. This airborne contagious disease caused more than nine million new cases annually, making TB the second leading cause of death after human immunodeficiency virus (HIV) infection [3].…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Immunoassay for Naked Eye Detection of Mycobacterium tuberculosis

et al. 2020

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Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Detection and control of infectious diseases is a major problem, especially in developing countries. Lateral flow immunoassay (LFIA) has been introduced as a handheld immunoassay-based point-of-care platform for an automated detection of TB. The CFP10-ESAT6 antigen of M. tuberculosis was used as the target in early detection of TB using LFIA strip-based POC strategy. An interesting platform based on optical signals is implemented as a colour change in the detection area that is visible to the naked eye. The gold nanoparticles (AuNPs) were used as the colour probe for the detection of a target of interest. The high-resolution transmission electron microscopy (HRTEM) image and ultraviolet-visible spectrophotometer (UV-Vis) analysis confirmed that the synthesized AuNPs were appropriate for the immunoassay designed. The platform consists of AuNPs conjugated with specific antibodies (Ab) to capture the antigen of M. tuberculosis. Under the capillary effect, sandwich immunoreactions of AuNP-Ab-antigen were performed on the test pad of the immunostrip, which can be observed by the colour signal on the test line of the strip with a short assay time. Furthermore, the newly developed biosensor was utilized in CFP10-ESAT6 antigen detection in human sputum specimens with satisfactory results. The characteristic coloured bands enable visual detection (naked eye) of target analyte without instrumentation. This noninvasive diagnose system which is sputum-based detection could provide user-friendly and affordable diagnostic tests in developing countries.

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Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform

Cited by 68 publications

References 43 publications

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis

Lateral Flow Immunoassay for Naked Eye Detection of Mycobacterium tuberculosis

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