Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode

Serafín, Verónica; Hernández, Pedro; Agüı́, L.; Yáñez‐Sedeño, P.; Pingarrón, José M.

doi:10.1016/j.electacta.2013.03.005

Cited by 40 publications

(20 citation statements)

References 27 publications

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“…As was reported earlier by Almeida et al [6], since Clark glucose sensor [7], this system was improved highly [6][7][8][9], and novel sensing systems for detection i.e. urea [10], creatinine [11], cholesterol [12], insulin [13], cancer cells [14] were developed. Electrochemical biosensors were also found for determination of microorganisms as Escherichia coli [15] or Salmonella typhimurium [16].…”

Section: Introductionmentioning

confidence: 81%

Electrochemical and Optical Biosensors in Medical Applications

Sołoducho¹,

Cabaj²

2015

Biosensors - Micro and Nanoscale Applications

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Abstract"nalysis of many biochemical processes is of great significance for clinical, biological, food, environmental as well as bioterror applications. "ut, exchanging of the biochemical information to kind of electronic signal is a defiance due to connecting an electronic tool directly to a biological surrounding. Electrochemical detection instrument due to its advantageous to analyze the subject of a biological sample has a great potential in conversion of a biochemical occurrence to an electronic signal.In this chapter we presented the advancements made in the field of electrochemical biosensors targeted at medical field as well as optical elements used in modern biosensors. This material encompasses the technology, performance and commercialization of this brood of sensors. We have focused on the future development perspectives of this class of sensors, based on the experience gathered by different groups researching this field.

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Section: Introductionmentioning

confidence: 81%

Electrochemical and Optical Biosensors in Medical Applications

Sołoducho¹,

Cabaj²

2015

Biosensors - Micro and Nanoscale Applications

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“…The voltage of -0.3 V in the β-CD-Fc-OH/SPE is lower than for many other amperometric sensors. 30,31 Also, we investigated the direct reduction of H2O2 at -0.3 V. When 100 μM H2O2 was added to the β-CD-Fc-OH/SPE, the reduction current increased significantly. This was mainly because H2O2 was reduced to H2O under the catalysis of HRP.…”

Section: Sensitivity Difference Between β-Cd-fc-oh/spe and Fc-oh/ Spementioning

confidence: 99%

“…Usually, ferrocene and horseradish peroxidase (HRP) were both introduced to minimize such interference. Kinoshita et al 30 designed a sensor with peroxidase entrapped on a Fc embedded carbon paste, which decreased the potential to 0.1 V. The sensor of Serafín et al 31 just needed 0.0 V to work with HRP and ferrocene. Harada and colleague prepared and studied the cyclodextrin-ferrocene inclusion complexes.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Detection of Serum Creatinine Based on β-Cyclodextrin-Ferrocenylmethanol Modified Screen-printed Electrode

Liu

et al. 2019

ANAL. SCI.

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Ferrocenylmethanol (Fc-OH) is included in β-cyclodextrin (β-CD) to form the β-CD-Fc-OH complex by host-guest supramolecular interaction. β-CD dissociates from the β-CD-Fc-OH complex due to the conversion of Fc-OH to Fc +-OH under a stimulus of oxidant. In our study, Fc-OH is oxidized after a series of enzymatic reactions of creatinine, which blocks the other means for oxidation of Fc-OH. And the background noise is reduced for testing for serum creatinine (sCr). The chronoamperometry signal for creatinine (with a constant potential-0.3 V vs. Ag/AgCl) increases linearly in the 1-1000 μM range, with a limit of detection as low as 0.5 μM. The amperometric potential of-0.3 V greatly prevents the interference of various redox substances in serum. The biosensor was used to test 120 clinical specimens and the results showed a linear correlation with the biochemical analyzer (R 2 = 0.9885). The biosensor could be applied to clinical trials and offers good prospects for clinical sCr detection.

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“…When one enzyme reaction cannot be effectively coupled with electrode reactions, multiple enzymes that work in a cascade mode are required. In this case, immobilization and colocalization of multiple enzymes are necessary for sensor fabrication, and various methods for immobilization and colocalization of multi-enzyme have been reported [ 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Development Perspective of Bioelectrocatalysis-Based Biosensors

Adachi

Kitazumi

Shirai

et al. 2020

Sensors

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Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.

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Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode

Cited by 40 publications

References 27 publications

Electrochemical and Optical Biosensors in Medical Applications

Electrochemical and Optical Biosensors in Medical Applications

Sensitive Detection of Serum Creatinine Based on β-Cyclodextrin-Ferrocenylmethanol Modified Screen-printed Electrode

Development Perspective of Bioelectrocatalysis-Based Biosensors

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