Sensitive Detection of Serum Creatinine Based on β-Cyclodextrin-Ferrocenylmethanol Modified Screen-printed Electrode

Abstract

Ferrocenylmethanol (Fc-OH) is included in β-cyclodextrin (β-CD) to form the β-CD-Fc-OH complex by host-guest supramolecular interaction. β-CD dissociates from the β-CD-Fc-OH complex due to the conversion of Fc-OH to Fc +-OH under a stimulus of oxidant. In our study, Fc-OH is oxidized after a series of enzymatic reactions of creatinine, which blocks the other means for oxidation of Fc-OH. And the background noise is reduced for testing for serum creatinine (sCr). The chronoamperometry signal for creatinine (with a constant potential-0.3 V vs. Ag/AgCl) increases linearly in the 1-1000 μM range, with a limit of detection as low as 0.5 μM. The amperometric potential of-0.3 V greatly prevents the interference of various redox substances in serum. The biosensor was used to test 120 clinical specimens and the results showed a linear correlation with the biochemical analyzer (R 2 = 0.9885). The biosensor could be applied to clinical trials and offers good prospects for clinical sCr detection.

Simple and sensitive electrochemical sensing of amethopterin by using carbon nanobowl/cyclodextrin electrode

“…As a simple oligosaccharide, β-cyclodextrins (β-CD) is considerable cheap and can be used to improve the water-solubility and dispersibility of the β-CD-based nanohybrids as the solubilizer [ [36] , [37] , [38] , [39] , [40] ]; meanwhile, the β-CD molecule exhibits excellent molecule recognition and accumulation capability [ [41] , [42] , [43] , [44] ]. Thus in this work, the nanohybrids composed of CNB and β-CD (CNB-CD) was prepared with a simple way for the first time ( Scheme 1 ) and dedicated to fabricate a low-cost, simple, and highly sensitive electrochemical sensing platform of ATP.…”

Highly sensitive determination of anesthetic drug benzocaine based on hydroxypropyl-β-cyclodextrin–carbon black nanohybrids

“…28 Importantly, the b-CD molecules can selectively bind with numerous organic targets into the cavities, exhibiting excellent molecule recognition and preconcentration capability. [29][30][31] Most recently, a CB/b-CD nanocomposite was prepared and used for the electroanalysis of utamide and nitrophenol; 32 the experiments demonstrated that CB/b-CD can show the collaborative advantages of CB (e.g., excellent electrical properties, large surface area) and b-CD (e.g., high molecule recognition and preconcentration capabilities). Inspired through these insights, a hydroxypropyl-b-cyclodextrin-CB (HP-b-CD-CB) nanohybrid was prepared herein and then used for the electroanalysis of BZC.…”

Electrochemical Enzyme-free Sensing of Oxalic Acid Using an Amine-mediated Synthesis of CuS Nanosphere

“…However, simple fabrication of nanomaterials with inexpensive and attractive properties for OA detection is still lacking. Among the different types of nanomaterials, transition metal based materials are playing key role and applied in many applications due to their good electronic conductivity, low cost, easy preparation [20,21]. In the past, copper sulfide (CuS) of special structural property was used for various electrochemical sensor applications.…”