Electrochemical Biosensor Based on Surface Imprinting for Zika Virus Detection in Serum

Abstract

Zika virus (ZIKV) is a flavivirus that was first identified in 1947. Initially, the virus was of little concern for health authorities given there were very few casualties among those suffering an infection. As such, only limited studies were performed on ZIKV. Recently, the viral infection has been linked to microcephaly in infants, which has prompted a dramatic increase in scientific interest in ZIKV research, including methods to allow for rapid virus identification. In this work we report the development of a new type of ZIKV electrochemical biosensor based on surface imprinted polymers and graphene oxide composites. The biosensor was used to detect ZIKV by measuring changes in the electrical signal with changing virus concentrations in buffer and serum using standard electrochemical techniques. The detection limit of our method is similar to the detection limit of the real-time quantitative reverse transcription PCR method.

Molecularly Imprinted Polymers and Surface Imprinted Polymers Based Electrochemical Biosensor for Infectious Diseases

“…Deposition and spin coating are two simple methods for preparing MIPs/SIPs modified electrode. Tancharoen et al [52] used spin coating method to prepare a SIPs for Zika virus (ZIKV) detection. In their procedures, a certain amount of the prepolymer−graphene oxide mixture was coated on a 1 × 1 cm 2 gold electrode before spinning at 1000 rpm for 10 s to remove excess prepolymer.…”

“…UV light-induced polymerization also can be used to prepare MIPs/SIPs on the electrode. It has been used to prepare SIPs-graphene oxide composites on the electrode for Zika virus (ZIKV) detection ( Figure 5B) [52]. Idil et al fabricated SIPs under UV-polymerization for E. coli detection [57].…”

“…As a member of the Flaviviridae virus family, Zika virus usually infects human beings and typically causes a skin rash, conjunctivitis, red eyes, malaise, muscle and joint pain, headache, or mild fever. Recently, Tancharoen et al [52] developed an electrochemical sensor based on SIPs and graphene oxide composite for Zika virus detection. The sensor was applied to detect virus in both PBS solutions and serum.…”

Application of Conducting Polymer Nanostructures to Electrochemical Biosensors

“…Recently, there is an increasing interest in developing a rapid Zika virus identification assay due to the appearance of the viral infection in infants. Tancharoen et al report the development of a new type of ZIKV electrochemical biosensor [44]. This biosensor consisted of surface imprinted polymers (SIPs)/graphene oxide composites, as shown in Figure 3c.…”

“…(c) Scheme depicting surface imprinted polymers (SIPs)-graphene oxide composites preparation on a gold surface for making the ZIKV biosensor. Reproduced with permission[44]. Copyright 2019 ACS publication.…”