Electrochemical Biosensor Based on Laser-Induced Graphene for COVID-19 Diagnosing: Rapid and Low-Cost Detection of SARS-CoV-2 Biomarker Antibodies

Oliveira, Marcely Echeverria; Lopes, Bruno Vasconcellos; Rossato, Jéssica Helisa Hautrive; Maron, Guilherme Kurz; Gallo, Betty Braga; Rosa, Andrei Borges La; Balboni, Raphael D. C.; Alves, Mariliana Luiza Ferreira; Ferreira, Marcos Roberto Alves; Pinto, Luciano da Silva; Conceição, Fabrício Rochedo; Piva, Evandro; Pereira, Cláudio M. P.; Escote, M. T.; Carreño, Neftalí Lênin Villarreal

doi:10.3390/surfaces5010012

Cited by 18 publications

(5 citation statements)

References 36 publications

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“…The electrochemical evaluation of the proposed biosensor using DPV resulted in linear dynamic detection behavior with wide concentration range of 1.0 × 105–0.1 cell/mL and LOD of ∼0.02 cell/mL. Other graphene-based electrochemical biosensors include identification of SARS-CoV-2 using dCas9 [ 99 ], determination of testosterone [ 100 ], determination of ascorbic acid, dopamine and uric acid [ 101 ], and detection of SARS-CoV-2 specific antigen using disposable electrochemical biosensor containing laser-induced graphene [ 102 ] and buffer-based zinc oxide/reduced graphene oxide nanosurface for the detection of SARS-CoV-2 antigens [ 103 ].…”

Section: Hyphenationmentioning

confidence: 99%

Smart Graphene-Based Electrochemical Nanobiosensor for Clinical Diagnosis: Review

Irkham

Ibrahim²,

Pwavodi

et al. 2023

Sensors

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The technological improvement in the field of physics, chemistry, electronics, nanotechnology, biology, and molecular biology has contributed to the development of various electrochemical biosensors with a broad range of applications in healthcare settings, food control and monitoring, and environmental monitoring. In the past, conventional biosensors that have employed bioreceptors, such as enzymes, antibodies, Nucleic Acid (NA), etc., and used different transduction methods such as optical, thermal, electrochemical, electrical and magnetic detection, have been developed. Yet, with all the progresses made so far, these biosensors are clouded with many challenges, such as interference with undesirable compound, low sensitivity, specificity, selectivity, and longer processing time. In order to address these challenges, there is high need for developing novel, fast, highly sensitive biosensors with high accuracy and specificity. Scientists explore these gaps by incorporating nanoparticles (NPs) and nanocomposites (NCs) to enhance the desired properties. Graphene nanostructures have emerged as one of the ideal materials for biosensing technology due to their excellent dispersity, ease of functionalization, physiochemical properties, optical properties, good electrical conductivity, etc. The Integration of the Internet of Medical Things (IoMT) in the development of biosensors has the potential to improve diagnosis and treatment of diseases through early diagnosis and on time monitoring. The outcome of this comprehensive review will be useful to understand the significant role of graphene-based electrochemical biosensor integrated with Artificial Intelligence AI and IoMT for clinical diagnostics. The review is further extended to cover open research issues and future aspects of biosensing technology for diagnosis and management of clinical diseases and performance evaluation based on Linear Range (LR) and Limit of Detection (LOD) within the ranges of Micromolar µM (10−6), Nanomolar nM (10–9), Picomolar pM (10–12), femtomolar fM (10–15), and attomolar aM (10–18).

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Section: Hyphenationmentioning

confidence: 99%

Smart Graphene-Based Electrochemical Nanobiosensor for Clinical Diagnosis: Review

Irkham

Ibrahim²,

Pwavodi

et al. 2023

Sensors

View full text Add to dashboard Cite

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“…It was further incubated in a mixture containing polystreptavidin‐HRP complex and TMB. The current was analysed using CV [114] …”

Section: Voltammetric Sensors For Diagnosis Of Sars‐cov‐2 and Its Bio...mentioning

confidence: 99%

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

2023

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The recent events of outbreaks of corona virus caused by the Severe Acute Respiratory Syndrome Coronavirus −2(SARS‐CoV‐2) raised a concern and increased urge for quick and rapid detection techniques of disease causing pathogens. The detection of the virus with high sensitivity and accuracy holds great importance. Nowadays, the most commonly employed technique is the Quantitative Reverse Transcription Polymerase Chain Reaction test (qRT‐PCR) and antigen test. While RT‐PCR technique is time consuming, expensive and labour intensive, antigen tests though simple, can often give false positive and false negatives. In this context, electrochemical biosensors developed in recent times have been identified as a potential strategy to overcome the limitations of these common techniques. This review article summarizes the current advancements in the field of voltammetric sensors for the detection of COVID‐19 virus and various biomarkers associated with it.

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“…On the other hand, electrochemical sensors provide better performances with direct electrical signals (i.e., current or impedance changes) that require minimal signal processing. For example, graphene-based electrochemical sensors achieved detection limits on the order of ≈10 fM for anti-SARS-CoV-2 antibodies ( Torrente-Rodríguez et al, 2020 ; Ali et al, 2021 ; Yakoh et al, 2021 ; Mattioli et al, 2022 ; Oliveira et al, 2022 ). However, the fabrication procedures are sometimes complex, and require high spatial resolution and temperature control.…”

Section: Introductionmentioning

confidence: 99%