Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer

Bezerra, Giselda; Córdula, C.; Campos, Danielly; Nascimento, G.A.; Oliveira, Natália; Seabra, Maria Aparecida Barreto Lopes; Visani, Valeria; Lucas, Sampaio; Lopes, I. D. S; Santos, Joana Dos; Xavier, Francisco; Borba, Maria Amélia Carlos Souto Maior; Martins, Danyelly Bruneska Gondim; Lima-Filho, José Luiz de

doi:10.1007/s00216-019-02040-5

Cited by 30 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“… Bezerra et al, 2019 ; Hideshima et al, 2018 ; Ianeselli et al, 2014 ; Jarczewska et al, 2016 ; Kiew et al, 2021 ; Kongsuphol et al, 2014 ; Macdonald et al, 2019 ; Ocaña et al, 2014 ; Wang et al, 2018 .…”

Section: Uncited Referencesunclassified

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Ramanathan

Ismail

Arshad

et al. 2022

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Section: Uncited Referencesunclassified

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Ramanathan

Ismail

Arshad

et al. 2022

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…The analytical signal referred to the current signal derived from the methylene blue redox indicator that intercalated within the double-stranded structure of aptamer. The proposed aptasensor allowed for HER2 detection in the range from 10-60 µg•L −1 with an LOD of 3 µg•L −1 [111].…”

Section: Carcinoembryonic Antigenmentioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

View full text Add to dashboard Cite

This paper focuses on the current state of art as well as on future trends in electrochemical aptasensors application in medical diagnostics. The origin of aptamers is presented along with the description of the process known as SELEX. This is followed by the description of the broad spectrum of aptamer-based sensors for the electrochemical detection of various diagnostically relevant analytes, including metal cations, abused drugs, neurotransmitters, cancer, cardiac and coagulation biomarkers, circulating tumor cells, and viruses. We described also possible future perspectives of aptasensors development. This concerns (i) the approaches to lowering the detection limit and improvement of the electrochemical aptasensors selectivity by application of the hybrid aptamer–antibody receptor layers and/or nanomaterials; and (ii) electrochemical aptasensors integration with more advanced microfluidic devices as user-friendly medical instruments for medical diagnostic of the future.

show abstract

“…Human epidermal growth factor receptor 2 (HER2) protein is a breast cancer biomarker in the form of three domains; a transmembrane region, an intracellular tyrosine kinase domain, and an extracellular domain that can be released into the bloodstream [3][4][5]. The HER2 protein concentration in serum is less than 15 ng mL -1 (4-14 ng mL -1 ) in healthy women and overexpressed in active surveillance of patients at risk or in treatment at about > 15 ng mL -1 [6][7].…”

Section: ■ Introductionmentioning

confidence: 99%

“…A homemade screen-printed electrode modified with poly-L-lysine has been used for immobilizing HER2 aptamer by electrostatic adsorption. The biosensor exhibited an excellent interaction between the aptamer and the target protein [3]. HER2 aptamer was immobilized by a selfassembled monolayer (SAM) on the surface of the gold electrode (GE), which was pretreated with 3mercaptopropionic acid (MPA), and the sensor was able to detect HER2 concentrations ranging from 10 -5 -10 2 ng mL -1 by using electrochemical impedance spectroscopy technique [19].…”

Section: ■ Introductionmentioning

confidence: 99%

An Electrochemical Aptasensor for the Detection of HER2 as a Breast Cancer Biomarker Based on Gold Nanoparticles-Aptamer Bioconjugates

et al. 2021

View full text Add to dashboard Cite

Inaccurate diagnoses contributes to the high mortality rate of breast cancer. Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast cancer tumors at around 20–30%. This study aims to develop an electrochemical biosensor for HER2 based on a gold nanoparticle-aptamer bioconjugate (AuNP@HER2 aptamer) and investigate the interaction between DNA aptamer and HER2 using computational methods. The bioconjugate was synthesized using maleimide and polyethylene glycol as a linker. The –NH2 group of cysteamine that modified the gold electrode can form a covalent bond with the bioconjugate maleimide. The interaction of the bioconjugated aptamer with HER2 was measured electrochemically based on the [Fe(CN)6]3−/4− redox system. The limit of detection, the linear range of HER2, precision, and accuracy in this study were 1.52 ng mL–1, 0.01 to 15.0 ng mL–1, 0.1298, and 94.06%, respectively. The structure of the DNA aptamer was modeled using mFold, Assemble2, and Chimera, with the interaction between the DNA aptamer and HER2 explored by NPDock. The modeling of the aptamer with HER2 showed that electrostatic interactions dominated the attractive forces. The resulting interaction pattern can be used as a template to improve the binding energy of the aptamer, thus providing insight into the development of aptamer-based biosensors.

show abstract

Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer

Cited by 30 publications

References 35 publications

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

An Electrochemical Aptasensor for the Detection of HER2 as a Breast Cancer Biomarker Based on Gold Nanoparticles-Aptamer Bioconjugates

Contact Info

Product

Resources

About