Electrochemical Aptasensor Based on Proximity-Dependent Surface Hybridization Assay for Single-Step, Reusable, Sensitive Protein Detection

Zhang, Yanli; Huang, Yong; Jiang, Jian‐Hui; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1021/ja0773047

Cited by 193 publications

(80 citation statements)

References 27 publications

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“…However, this classification "signal-on" and "signal-off", is also often employed in the sandwich assays (Centi et al, 2007(Centi et al, , 2009Zhang et al, 2007Zhang et al, , 2009 or label-free aptasensors, when an increase or decrease of the signal is observed after the formation of the aptamer-target complex (Cheng et al, 2007;Degefa and Kwak, 2008;Meirinho et al, 2015;Rodríguez and Rivas, 2009).…”

Section: Design Strategiesmentioning

confidence: 99%

“…Some works have reported the use of the sandwich-format aptasensor for the detection of different proteins, namely thrombin (Centi et al, 2007;Ding et al, 2010;Kang et al, 2008;Zhao et al, 2011a), C-reactive protein (CRP) (Centi et al, 2009) and PDGF Zhang et al, 2007Zhang et al, , 2009 . Centi et al (2007) developed a labelled "signal-on" electrochemical aptasensor for the detection of thrombin in complex matrices using the sandwich assay with magnetic particles as solid supports.…”

Section: Aptasensors Based On Sandwich Designmentioning

confidence: 99%

“…Again very low detection limits (2 nM) in serum samples were obtained (Centi et al, 2009). A similar sandwich strategy was designed by Zhang et al (2007Zhang et al ( , 2009 to detect the proteins PDGF-BB and PDGF, respectively. In these works, the detection of the protein targets was based on the proximity-dependent surface hybridization assay.…”

Section: Aptasensors Based On Sandwich Designmentioning

confidence: 99%

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Voltammetric aptasensors for protein disease biomarkers detection: A review

Meirinho

Dias

Peres

et al. 2016

Biotechnology Advances

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An electrochemical aptasensor is a compact analytical device where the bioreceptor (aptamer) is coupled to a transducer surface to convert a biological interaction into a measurable signal (current) that can be easily processed, recorded and displayed. Since the discovery of the Systematic Evolution of Ligands by Enrichment (SELEX) methodology, the selection of aptamers and their application as bioreceptors has become a promising tool in the design of electrochemical aptasensors. Aptamers present several advantages that highlight their usefulness as bioreceptors such as chemical stability, cost effectiveness and ease of modification towards detection and immobilization at different transducer surfaces. In this review, a special emphasis is given to the potential use of electrochemical aptasensors for the detection of protein disease biomarkers using voltammetry techniques. Methods for the immobilization of aptamers onto electrode surfaces are discussed, as well as different electrochemical strategies that can be used for the design of aptasensors.

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Section: Design Strategiesmentioning

confidence: 99%

Section: Aptasensors Based On Sandwich Designmentioning

confidence: 99%

Section: Aptasensors Based On Sandwich Designmentioning

confidence: 99%

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Voltammetric aptasensors for protein disease biomarkers detection: A review

Meirinho

Dias

Peres

et al. 2016

Biotechnology Advances

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“…More specifically, the insight into conformational switches of aptamers on interacting with cognate ligands has become a unique mechanism in the design of electrochemical aptasensors [28 -36]. Recently we reported a new strategy of proximity-dependent surface hybridization assay for the development of electrochemical aptasensor for protein detection [37]. This assay was based on the simultaneous recognition of a single protein molecule at two different sites by a pair of aptamer probes, which promoted two ferrocene-tagged tail sequences of the proximate aptamer pair to be cooperatively annealed on the capture probes immobilized on an electrode surface with redox current thus triggered.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor Based on Proximity‐Dependent Surface Hybridization Assay for Protein Detection

et al. 2009

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Sensitive and selective detection of proteins is of great importance in biomedical research and clinical diagnosis. Proximity-dependent surface hybridization assay affords a promising platform for specific and ultrasensitive electrochemical detection of proteins. An improved design of proximity-dependent surface hybridization assay is developed. A pair of aptamer probes that recognize different sites of target platelet-derived growth factor protein (PDGF) are designed with a short complementary sequences. Simultaneous recognition of a single PDGF molecule by the aptamer pair enables the hybridization between the complementary sequences of both aptamer probes, which keeps two tail sequences of both aptamer probes in close proximity and promotes cooperative annealing of the aptamer-protein-aptamer sandwiched complex on capture probes immobilized on the electrode. Thus, ferrocene tag at the terminus of one aptamer probe is drawn in close contact with the electrode with strong redox signal readily obtained in voltammetric reading. The aptasensor is shown to have high sensitivity with a detection limit as low as 0.1 pg mL À1 and excellent specificity with resistance to interference from coexisting proteins.

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“…A variety of techniques have been introduced for the construction of sensitive electrochemical immunosensors based on enzyme-catalyzed signal amplification or transduction mechanisms (4, 10 -15 ), as well as nanoscale carriers or reporters including metal or semiconductor nanoparticles (16)(17)(18)(19)(20)(21). Biological amplification based on nucleic acid labels has also been shown to provide enhanced electrochemical detection of proteins (15,22 ). Most of these electrochemical signal amplification strategies have remained at the laboratory-development level, with the analytical protocols comprising skill-demanding operations and specifically synthesized labels.…”

mentioning

confidence: 99%

Prostate Specific Antigen Detection Using Microgapped Electrode Array Immunosensor with Enzymatic Silver Deposition

et al. 2009

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Background: Analysis of trace proteins plays an essential role in the fields of biomedical research and clinical diagnosis. Development of methods for the detection of proteins at very low concentrations has historically been a challenge in immunochemistry. We have developed an electrical immunosensor for the detection of prostate specific antigen (PSA). Methods: The electrical immunosensor uses a microgapped interdigitated electrode array (MGIDEA) based on enzymatic silver deposition reaction. The deposition of silver was dispersed over the microgaps and allows the microgapped interdigitated electrodes to be electrically connected, resulting in an increase in electrical conductance of MGIDEA that is used to quantify the analyte concentration. We used this electrical immunosensor to measure PSA in human serum samples from patients with prostate diseases. Results: This electrical immunosensor exhibited a linear response with PSA concentrations over a 6-decade range from 1.0 pg/L to 1.0 μg/L, with detection limit of 0.9 pg/L. PSA concentrations using this immunosensor agreed within 10% of those obtained using a commercial chemiluminescent immunoassay. Conclusions: The MGIDEA method has characteristics (analyte specific, low background, low limit of detection) that provide potential for molecular detection in various biomedical areas.

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Electrochemical Aptasensor Based on Proximity-Dependent Surface Hybridization Assay for Single-Step, Reusable, Sensitive Protein Detection

Cited by 193 publications

References 27 publications

Voltammetric aptasensors for protein disease biomarkers detection: A review

Voltammetric aptasensors for protein disease biomarkers detection: A review

Electrochemical Aptasensor Based on Proximity‐Dependent Surface Hybridization Assay for Protein Detection

Prostate Specific Antigen Detection Using Microgapped Electrode Array Immunosensor with Enzymatic Silver Deposition

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