Electrochemical aptasensor based on anisotropically modified (Janus-type) gold nanoparticles for determination of C-reactive protein

Villalonga, Anabel; Sánchez, Alfredo; Vilela, Diana; Mayol, Beatriz; Martı́nez-Ruiz, Paloma; Villalonga, Reynaldo

doi:10.1007/s00604-022-05420-4

Cited by 8 publications

(2 citation statements)

References 35 publications

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“…Thus, the developed sensor has the potential for the analysis of the CRP concentration in healthy individuals (≤1000 ng/mL) or patients (>1000 ng/mL, through dilution) [55]. Table 1 summarized the CRP detection performance using various sensors [56][57][58][59][60][61][62]. Compared to other sensors, the fabricated ECL aptasensor exhibited a wider linear detection range and lower DL.…”

Section: Ecl Determination Of Crpmentioning

confidence: 99%

The Fabrication of a Probe-Integrated Electrochemiluminescence Aptasensor Based on Double-Layered Nanochannel Array with Opposite Charges for the Sensitive Determination of C-Reactive Protein

Li,

Han,

2023

Molecules

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The effective and sensitive detection of the important biomarker, C-reactive protein (CRP), is of great significance in clinical diagnosis. The development of a convenient and highly sensitive electrochemiluminescence (ECL) aptasensor with an immobilized emitter probe is highly desirable. In this work, a probe-integrated ECL aptamer sensor was constructed based on a bipolar silica nanochannel film (bp-SNF) modified electrode for the highly sensitive ECL detection of CRP. The bp-SNF, modified on an ITO electrode, consisted of a dual-layered SNF film, including the negatively charged inner SNF (n-SNF) and the outer SNF with a positive charge and amino groups (p-SNF). The ECL emitter, tris(bipyridine) ruthenium (II) (Ru(bpy)32+), was stably immobilized in a nanochannel of bp-SNF using the dual electrostatic interactions with n-SNF attracting and p-SNF repelling. The amino groups on the outer surface of bp-SNF were aldehyde derivatized, allowing for the covalent immobilization of recognitive aptamers (5′-NH2-CGAAGGGGATTCGAGGGGTGATTGCGTGCTCCATTTGGTG-3′), leading to the recognition interface. When CRP bound to the aptamer on the recognition interface, the formed complex increased the interface resistance and reduced the diffusion of the co-reactant tripropylamine (TPA) into the nanochannels, leading to a decrease in the ECL signal. Based on this mechanism, the constructed aptamer sensor could achieve a sensitive ECL detection of CRP ranging from 0.01 to 1000 ng/mL, with a detection limit (DL) of 8.5 pg/mL. The method for constructing this probe-integrated ECL aptamer sensor is simple, and it offers a high probe stability, good selectivity, and high sensitivity.

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Section: Ecl Determination Of Crpmentioning

confidence: 99%

The Fabrication of a Probe-Integrated Electrochemiluminescence Aptasensor Based on Double-Layered Nanochannel Array with Opposite Charges for the Sensitive Determination of C-Reactive Protein

Li,

Han,

2023

Molecules

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“…This work can be used for the determination of serum samples. Recently, this group reported the preparation of a novel Janus nanoparticle based on asymmetrically functionalized gold colloids by using partially masked Fe 3 O 4 @SiO 2 core-shell nanoparticles (nanocapsules) as masking tools and topologically selective surface modification of polyamide amine (PAMAM) dendrites as the primary capping material [ 158 ]. Then, horseradish peroxidase (HRP) was immobilized on the dendritic surface as a signal oxidoreductase.…”

Section: Janus Particles For Electrochemical Poctmentioning

confidence: 99%

Application of Janus Particles in Point-of-Care Testing

et al. 2022

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Janus particles (JPs), named after the two-faced Roman god, are asymmetric particles with different chemical properties or polarities. JPs have been widely used in the biomedical field in recent years, including as drug carriers for targeted controlled drug release and as biosensors for biological imaging and biomarker detection, which is crucial in the early detection and treatment of diseases. In this review, we highlight the most recent advancements made with regard to Janus particles in point-of-care testing (POCT). Firstly, we introduce several commonly used methods for preparing Janus particles. Secondly, we present biomarker detection using JPs based on various detection methods to achieve the goal of POCT. Finally, we discuss the challenges and opportunities for developing Janus particles in POCT. This review will facilitate the development of POCT biosensing devices based on the unique properties of Janus particles.

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PEG-functionalized black phosphorus quantum dots as stable and biocompatible electrochemiluminescence luminophores for sensitive detection of tumor biomarker

Liu

Sun

et al. 2023

Microchim Acta

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Electrochemical aptasensor based on anisotropically modified (Janus-type) gold nanoparticles for determination of C-reactive protein

Cited by 8 publications

References 35 publications

The Fabrication of a Probe-Integrated Electrochemiluminescence Aptasensor Based on Double-Layered Nanochannel Array with Opposite Charges for the Sensitive Determination of C-Reactive Protein

The Fabrication of a Probe-Integrated Electrochemiluminescence Aptasensor Based on Double-Layered Nanochannel Array with Opposite Charges for the Sensitive Determination of C-Reactive Protein

Application of Janus Particles in Point-of-Care Testing

PEG-functionalized black phosphorus quantum dots as stable and biocompatible electrochemiluminescence luminophores for sensitive detection of tumor biomarker

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