Electrochemical analysis of nucleic acids as potential cancer biomarkers

Bartošík, Martin; Jiráková, Ludmila

doi:10.1016/j.coelec.2019.01.002

Cited by 34 publications

(24 citation statements)

References 51 publications

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“…Currently, the main avenues to assess methylation sites in nucleic acids include: sodium bisulfite modification, sequence‐specific enzyme digestion and affinity recognition of epimarks .…”

Section: Conventional Methods To Detect Epigenetic Modifications Of Nmentioning

confidence: 99%

“…Currently, the main avenues to assess methylation sites in nucleic acids include: sodium bisulfite modification, sequence-specific enzyme digestion and affinity recognition of epimarks [36,37]. Sodium bisulfite modification (or bisulfite conversion) is one of the most useful tools for analysing 5-mC methylation.…”

Section: Conventional Methods To Detect Epigenetic Modifications Of Nmentioning

confidence: 99%

“…The main characteristics of the latest electrochemical methods described so far for the determination of non‐coding RNAs, some of them nicely reviewed recently , are summarized in Table .…”

Section: Electrochemical (Bio)sensing Of Nucleic Acid Epigeneticsmentioning

confidence: 99%

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Advances in Electrochemical (Bio)Sensing Targeting Epigenetic Modifications of Nucleic Acids

et al. 2019

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Nucleic acids epigenetic modifications including DNA and RNA methylation and post transcriptional gene regulation by noncoding RNAs (ncRNAs) such as microRNAs and long ncRNAs (lncRNAs), are of great relevance due to their role in mammalian development and the initiation and progression of various diseases like cancer. Over the past decades, a great research effort was made for determining nucleic acid methylation, electrochemical biosensors rapidly developing as efficient analytical tools for the determination of epigenetic modification of nucleic acids. In this review article, a critical overview of recently reported advances in their development and use for the determination of epigenetic modifications of nucleic acids, including the presence of methylated bases in DNA and RNA and the aberrant expression of microRNAs and lncRNAs, is provided. Special emphasis is made on methodologies successfully applied to real clinical samples, unmet challenges or key points toward future research.

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Section: Conventional Methods To Detect Epigenetic Modifications Of Nmentioning

confidence: 99%

Section: Conventional Methods To Detect Epigenetic Modifications Of Nmentioning

confidence: 99%

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Advances in Electrochemical (Bio)Sensing Targeting Epigenetic Modifications of Nucleic Acids

et al. 2019

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“…The turnaround time including LAMP is about 2.5 hours. This method was presented by Bartosik et al 171,172 for the detection of HPV types 16 and 18 on electrochemical chips using a multi potentiostat/galvanostat. The analytical sensitivity was described with a limit of detection of 0.1 ng DNA per reaction and a limit of quantication of 0.6 ng per reaction.…”

Section: Alternately Binding Quenching Probe Competitive Lamp (Abc-lamp)mentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Becherer

Borst

Bakheit³

et al. 2020

Anal. Methods

289

245

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“…cancer) to infectious (e.g. HIV, malaria and sepsis), as well as the presence of poisons like pesticides and is an important means for determining the presence of antibiotic resistance (Liu et al, 2019;Kumar, 2019;Diculescu, 2005; Bartosik and Jirakova, 2019). Combined with the benefits of microfabrication and lab-on-a-chip approaches, it is not surprising that electrochemical DNA sensors for biomedical applications are a popular area of research.…”

Section: Introductionmentioning

confidence: 99%

A review of microfabricated electrochemical biosensors for DNA detection

Blair

Corrigan

2019

Biosensors and Bioelectronics

125

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This review article presents an overview of recent work on electrochemical biosensors developed using microfabrication processes, particularly sensors used to achieve sensitive and specific detection of DNA sequences. Such devices are important as they lend themselves to miniaturisation, reproducible mass-manufacture, and integration with other previously existing technologies and production methods. The review describes the current state of these biosensors, novel methods used to produce them or enhance their sensing properties, and pathways to deployment of a complete point-ofcare biosensing system in a clinical setting.

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Electrochemical analysis of nucleic acids as potential cancer biomarkers

Cited by 34 publications

References 51 publications

Advances in Electrochemical (Bio)Sensing Targeting Epigenetic Modifications of Nucleic Acids

Advances in Electrochemical (Bio)Sensing Targeting Epigenetic Modifications of Nucleic Acids

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

A review of microfabricated electrochemical biosensors for DNA detection

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