Electroantennograms From Spruce Budworm Moths (Choristoneura Fumiferana) (Lepidoptera: Tortricidae) of Different Ages and for Various Pheromone Concentrations

Abstract

An electroantennogram (EAG) technique, which indicates electrochemical activity in a whole antenna, was used to study sex pheromone reception in spruce budworm moth antennae. For both males and females the EAG exhibited a phasic depolarization, reaching maximum near the end of a puff stimulation, followed immediately by repolarization which was prolonged by increasing amounts as the source concentration of the pheromone was increased. The dose–response curves for both sexes were sigmoid in shape, but they indicated that female antennae have a higher threshold and a lower peak response than that of males. Antennal response changed with age, being a regular increase and decrease for females and irregular for males.

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

“…In moths, increases in EAG amplitude have been reported during the final days of pupation (Payne, Shorey, and Gaston, 1970;Schweitzer et al, 1976) and during the first 2 days to 1 week of adult life (Payne et al, 1970;Ross, Palaniswamy, and Seabrook, 1979;Seabrook et al, 1979Seabrook et al, , 1987. A decrease in EAG amplitude in older insects, designated "senescence," has been described for moths (Ross et al, 1979;Seabrook et al, , 1987 and beetles (Dickens and Moorman, 1990). Our results in Drosophila, and the results of Schweitzer et al (1976) in Manduca, show that the kinetics with which insect EAGs change can vary for different odorants.…”

EFFECT OF A JUVENILE HORMONE ANALOGUE ON OLFACTORY SENSITIVITY OF EASTERN SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

“…To study the influence of JHA on the olfactory sensitivity of moths, male and female adults were treated with the hormone analogue on the day of their emergence (0 day old). As our previous observations (Palaniswamy et al, 1979) indicated that at least 10--15 hr had to elapse between treatment and recording before one could observe any significant reduction in the sex pheromone response, EAG responses of the treated moths to various odours were recorded on day 1. Results summarized in Table I indicate that JHA treatment significantly (t- (6) 0.59+_ 0.07 (7) control 1.08 ± 0.07 ( 11 ) 1.18_+ 0.09 (10) LF JHA treated 0.62_+ 0.06 ( I 1) 0.68 +-0.10 (10) Stimulus concentrations used at source.…”

“…The synthetic sex pheromone for spruce budworm, a 97:3 mixture of trans-and cis-I l-tetradecenai (Sanders & Weatherston, 1976), was obtained from Chemical Samples Co., Columbus, Ohio. EAG responses of male and female moths to this female sex pheromone and other volatile chemicals were obtained using the technique of Roeiofs & Comeau (1971) as modified by Ross et al (1979).…”

“…The presence of juvenile hormone (JH) in the adult insects of both sexes (Schooley, 1977) and its role in reproduction (De Wilde & De Loof, 1973) is well documented. Topical application of a juvenile hormone analogue (JHA) has been found to reduce the antennal sensitivity of female spruce budworm moths to their sex pheromone (Palaniswamy et al, 1979). However, it is not known whether the hormone analogue has a selective effect on the sensitivity of sex pheromone receptors or whether it affects the sensitivity of other olfactory receptors as well.…”

Autodetection in <i>Helicoverpa armigera</i> (Hubner)

“…In moth species only a few examples of autodetection have been recorded, viz. Choristoneura fumiferana (Clemens) 15 , Spodoptera littoralis (Boisduval) 16 , Cydia spp. 17 and Panaxia quadripunctaria (Poda) 14,18 .…”