Electroacupuncture Promotes the Differentiation of Transplanted Bone Marrow Mesenchymal Stem Cells Overexpressing TrkC into Neuron-Like Cells in Transected Spinal Cord of Rats

Ding, Ying; Yan, Qing; Ruan, Jing Wen; Zhang, Yan Qing; Li, Wen Jie; Zeng, Xiang; Huang, Si Fan; Zhang, Yu Jiao; Wu, Jin Lang; Fisher, Danny; Dong, Hongxin; Zeng, Yuan

doi:10.3727/096368912x655037

Cited by 27 publications

(51 citation statements)

References 101 publications

(127 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another is their release of MMPs that enzymatically degrade the chondroitin sulfate proteoglycan (CSPG) that is present in the ECM of denervated peripheral nerve Schwann cells, which inhibits axon regeneration [258][259][260]. Mesenchymal stem cells also cause the downregulation of CSPG synthesis in peripheral nerves, thus changing the ECM from one that inhibits to one that promotes axon regeneration [261].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Kuffler

2015

Mol Neurobiol

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) has been tested in vitro, in animal models, and clinically for its efficacy in enhancing the rate of wound healing, reducing pain associated with injuries, and promoting axon regeneration. Although extensive data indicate that PRP-released factors induce these effects, the claims are often weakened because many studies were not rigorous or controlled, the data were limited, and other studies yielded contrary results. Critical to assessing whether PRP is effective are the large number of variables in these studies, including the method of PRP preparation, which influences the composition of PRP; type of application; type of wounds; target tissues; and diverse animal models and clinical studies. All these variables raise the question of whether one can anticipate consistent influences and raise the possibility that most of the results are correct under the circumstances where PRP was tested. This review examines evidence on the potential influences of PRP and whether PRP-released factors could induce the reported influences and concludes that the preponderance of evidence suggests that PRP has the capacity to induce all the claimed influences, although this position cannot be definitively argued. Well-defined and rigorously controlled studies of the potential influences of PRP are required in which PRP is isolated and applied using consistent techniques, protocols, and models. Finally, it is concluded that, because of the purported benefits of PRP administration and the lack of adverse events, further animal and clinical studies should be performed to explore the potential influences of PRP.

show abstract

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Kuffler

2015

Mol Neurobiol

View full text Add to dashboard Cite

show abstract

“…The results indicated that TrkC acts as a chemokine receptor with its high affinity for NT-3 and may play a role in MSC homing. TrkC gene-modified MSCs transplantation combined with electroacupuncture treatment not only increased MSC survival and differentiation into neuron-like cells but also promoted CST regeneration across injured sites to the caudal cord and functional improvement in SCI (43). In addition, the conduction of cortical motorevoked potentials (MEPs) and hindlimb locomotor function increased as compared to controls.…”

Section: Trkcmentioning

confidence: 90%

Genetic modification of mesenchymal stem cells in spinal cord injury repair strategies

et al. 2013

View full text Add to dashboard Cite

“…NT-3-positive BM-MSC administration to the injured spinal cords facilitated axonal regeneration, promoted neuronal survival [225], and favoured remyelination and functional recovery in the demyelinated spinal cords of the studied animals [226]. NT-3 acts through tropomyosin-related kinase receptor tyrosine kinases (Trk) [227], a feature that was used for BM-MSC transduction with Trk type C (TrkC) gene overexpression [228]. TrkC-producing MSCs possessed the ability to prolong their survival after delivery, improve differentiation toward neuron-like cells, and participated in corticospinal tract regeneration in the injured areas, mainly due to the elevated concentration of NT-3.…”

Section: Mscs In Neural Differentiation and Repairmentioning

confidence: 99%

“…ecSOD [158] GLP-1 [160] HCN1 [19] HCN4 [186,187] Fn14 [189] Wnt11 [164] HO-1 [167,168] My GA NEURAL DIFFERENTIATION AND REPAIR BDNF [22] [201] [203,204] GDNF [20] [207] [216,217] NGF [236] NT-3 [225,226] MNTS-1 [230] Shh [232] VEGF [21] GLP-1 [215] VIP [221] IL-10 [222] interferon-β [223] CXCR4 [205] TrkC [228] Ng Ng RA Nu Ma KIDNEY REPAIR HGF [23] GSTM1 [267] Survivin [268] …”

Section: Cartilage Productionmentioning

confidence: 99%

Genetic Engineering of Mesenchymal Stem Cells for Regenerative Medicine

Nowakowski

Walczak

Janowski

et al. 2015

Stem Cells and Development

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs), which can be obtained from various organs and easily propagated in vitro, are one of the most extensively used types of stem cells and have been shown to be efficacious in a broad set of diseases. The unique and highly desirable properties of MSCs include high migratory capacities toward injured areas, immunomodulatory features, and the natural ability to differentiate into connective tissue phenotypes. These phenotypes include bone and cartilage, and these properties predispose MSCs to be therapeutically useful. In addition, MSCs elicit their therapeutic effects by paracrine actions, in which the metabolism of target tissues is modulated. Genetic engineering methods can greatly amplify these properties and broaden the therapeutic capabilities of MSCs, including transdifferentiation toward diverse cell lineages. However, cell engineering can also affect safety and increase the cost of therapy based on MSCs; thus, the advantages and disadvantages of these procedures should be discussed. In this review, the latest applications of genetic engineering methods for MSCs with regenerative medicine purposes are presented.

show abstract

Electroacupuncture Promotes the Differentiation of Transplanted Bone Marrow Mesenchymal Stem Cells Overexpressing TrkC into Neuron-Like Cells in Transected Spinal Cord of Rats

Cited by 27 publications

References 101 publications

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Genetic modification of mesenchymal stem cells in spinal cord injury repair strategies

Genetic Engineering of Mesenchymal Stem Cells for Regenerative Medicine

Contact Info

Product

Resources

About