Electroacupuncture Attenuates Limb Ischemia-Reperfusion-Induced Lung Injury Via p38 Mitogen-Activated Protein Kinase-Nuclear Factor Erythroid-2-Related Factor-2/Heme Oxygenase Pathway

Gong, Lirong; Kan, Yong-Xing; Liu, Yi; Dong, Shuying; Zhao, Dinghuan; Shi, Jia; Yu, Jianbo

doi:10.1016/j.jss.2019.08.021

Cited by 14 publications

(9 citation statements)

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“…Moreover, this kind of injury is not only limited to the limbs, but it can also impact more distant viscera. 12 When the reperfusion phase occurs, the injurious factors that have accumulated in the affected limb will flow throughout the whole body via the blood. The blood supply to the lung is very rich and it is an essential site for gaseous exchange with the outside world.…”

Section: Discussionmentioning

confidence: 99%

The mechanism of curcumin post-treatment relieving lung injuries by regulating miR-21/TLR4/NF-κB signalling pathway

Zou

Sun

2020

J Int Med Res

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Objective To investigate the mechanism by which curcumin prevents lung injury in a rat model of limb ischaemia-reperfusion injury. Methods Rats were randomized into four groups ( n = 20): control group (sham group); ischaemia-reperfusion group (I/R group); curcumin group (I/R+Cur group); and inhibitor of agomir-21 group (I/R+Cur+antagomir-21 group). At 3 h after reperfusion, lung tissues were collected for histopathology and immunohistochemistry to determine the apoptosis index (AI). Lung injury score (LIS) and lung wet/dry (W/D) ratio were determined. Lung microRNA-21 (miR-21) mRNA levels were measured using reverse transcription–polymerase chain reaction. Toll-like receptor 4 (TLR4) and nuclear factor kappa-B p65 (NF-κB p65) protein levels were measured by Western blot analysis. Tumour necrosis factor (TNF)-α and interleukin (IL)-1β levels were determined by enzyme-linked immunosorbent assays. Results In the I/R group, the W/D, LIS, AI, miR-21 mRNA, TLR4, NF-κB p65, TNF-α and IL-1β were significantly increased and the PaO2 was decreased compared with the sham group. Evidence of lung injury was observed in the I/R group and this was alleviated in the I/R+Cur group. An inhibitor of miR-21 (antagomir-21) reversed the protective effects of curcumin. Conclusion Curcumin post-treatment can alleviate the lung injuries induced by limb ischaemia-reperfusion via downregulating the levels of miR-21 mRNA.

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Section: Discussionmentioning

confidence: 99%

The mechanism of curcumin post-treatment relieving lung injuries by regulating miR-21/TLR4/NF-κB signalling pathway

Zou

Sun

2020

J Int Med Res

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“…However, the underlying mechanism remains unknown. Gong et al (12) demonstrated that ea attenuated limb I/R-induced lung injury via the p38 mitogen-activated protein kinase/nuclear factor erythroid 2-like 2/heme oxygenase 1 pathway. However, whether EA also reduces lung injury induced by limb I/R via the suppression the TLR4/NF-κB pathway has not yet been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture pre‑conditioning protects from lung injury induced by limb ischemia/reperfusion through TLR4 and NF‑κB in rats

Lou

et al. 2020

Mol Med Rep

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limb ischemia/reperfusion (i/r) can induce inflammation, causing acute lung injury. The Toll-like receptor 4 (Tlr4)/nF-κB pathway plays an important role in acute and chronic inflammatory disorders. Several studies have demonstrated the efficacy of acupuncture in lung inflammatory injury. The aim of the present study was to elucidate the mechanism underlying the protective effect of electroacupuncture (ea) against lung injury induced by limb i/r. ea applied at the Zusanli and Sanyinjiao acupoints attenuated lung injury and decreased the secretion of inf lammatory factors such as tumor necrosis factor-α, interleukin (il)-1, il-6 and myeloperoxidase. Moreover, the expression levels of TLR4 and NF-κB were suppressed by EA. Thus, the present findings suggested that EA can reduce pulmonary inflammation induced by limb I/R injury, possibly via the inhibition of the TLR4/NF-κB pathway.

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“…Evidence has revealed that electric stimulation at special acupoints could suppress the in ammasome activation and subsequently exert protective effects in the lungs [15,16]. Our previous study also con rmed that electroacupuncture could attenuate the lung injury during endotoxic shock or I/R injury, in which the up-regulated expression of HO-1 plays a crucial role [17,18]. But few researchers focus on I/R induced lung injury in patients undergoing lower extremity surgery and the underlying mechanism remains unclear.…”

Section: Introductionmentioning

confidence: 71%

“…Many clinical studies have documented that the electric acupuncture treatment increases pulmonary ventilation and the mechanism is related with its effect of relieving local in ammation and mitigating small airway stenosis [20,21]. Our previous research also demonstrated that electric stimulation at FeiShu (BL13) and ZuSanLi (ST36) acupoints could alleviate endotoxin shock-induced acute lung injury by reducing oxidative stress injury in rabbits [17]. In the traditional Chinese Medicine theory, ZuSanLi (ST36) acupoint is highly praised in health care and clinical treatment for modulating the Qi-blood balance.…”

Section: Discussionmentioning

confidence: 99%

Transcutaneous Electrical Acupoint Stimulation Attenuates Lung Injury Caused by Tourniquet-induced Limb Ischemia/reperfusion During Lower Extremity Surgery: A Randomized Controlled Trial

Gong

Zhao

et al. 2020

Preprint

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Background: Tourniquet-induced extremity ischemia-reperfusion injury is one of the major complications in patients with lower extremity surgery, while lung is the most vulnerable organ to insult subsequent to ischemia-reperfusion. Current scientific research data has shown that electric stimulation at special acupoints could suppress inflammasome activation and subsequently exert protective effects in the lungs. Thus, the effect of transcutaneous electrical acupuncture point stimulation (TEAS) on lung injury induced by limb ischemia/reperfusion remains to be elucidated. Methods: One hundred individuals scheduled for unilateral lower extremity surgery were randomly divided into 2 groups (n=50 each): transcutaneous electrical acupoint stimulation group (group T) and control group (group C). Patients in group T were given TEAS at FeiShu (BL13, bilateral) acupoints and ZuSanLi (ST36, non-surgical side) acupoint, while patients in group C received sham stimulation (no current) at the same acupoints for the same time as the TEAS group. Arterial blood samples were collected to assess blood gas analysis and other indicators. Results: The TEAS group showed significant improvements in blood gas analysis. Compared with group C, PaO2 and oxygenation index (OI) in group T were significantly increased at T4(4h after removing the tourniquet), while A-aDO2 and respiratory index (RI) were decreased at T4. There was an increase in the HO-1 levels in group T at T3(2h after removing the tourniquet) and T4 compared with group C. Compared with group T, SOD levels were signiﬁcantly lower at T4 in group C. At T4, the levels of ICAM-1, IL-6 and IL8 were significantly lower in group T when compared to group C. At T3 and T4, IL-10 levels were higher when compared to group C, while TNF-a levels were significantly lower.Conclusions: Transcutaneous electrical acupoint stimulation attenuates lung injury following tourniquet-induced extremity ischemia-reperfusion in patients, and HO-1 is endowed of potential mechanism of cytoprotective effects against tourniquet-induced lung injury. Trial registration: Chinese Clinical Trial Registry, ChiCTR-IOR-16008264. Registered 11 April 2016, http://www.chictr.org.cn/ ChiCTR-IOR-16008264

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Electroacupuncture Attenuates Limb Ischemia-Reperfusion-Induced Lung Injury Via p38 Mitogen-Activated Protein Kinase-Nuclear Factor Erythroid-2-Related Factor-2/Heme Oxygenase Pathway

Cited by 14 publications

References 50 publications

The mechanism of curcumin post-treatment relieving lung injuries by regulating miR-21/TLR4/NF-κB signalling pathway

The mechanism of curcumin post-treatment relieving lung injuries by regulating miR-21/TLR4/NF-κB signalling pathway

Electroacupuncture pre‑conditioning protects from lung injury induced by limb ischemia/reperfusion through TLR4 and NF‑κB in rats

Transcutaneous Electrical Acupoint Stimulation Attenuates Lung Injury Caused by Tourniquet-induced Limb Ischemia/reperfusion During Lower Extremity Surgery: A Randomized Controlled Trial

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