Electroacupuncture ameliorates beta-amyloid pathology and cognitive impairment in Alzheimer disease via a novel mechanism involving activation of TFEB (transcription factor EB)

Celastrol enhances transcription factor EB (TFEB)-mediated autophagy and mitigates Tau pathology: Implications for Alzheimer's disease therapy

“…Cells were lysed in RIPA to separate cytosol and nucleus extracts prepared according to our previous protocol 45 , 46 , 47 . The extracted protein was analyzed using Western blot, the protein was run in SDS-PAGE separating them at different kDa.…”

The Emerging Roles of Autophagy in Human Diseases

“…The relationship between Aβ and autophagy is complicated. First, Aβ is degraded through autophagy, and several studies show a decreased Aβ level in cells and improved cognitive ability in an AD mouse model when autophagy is induced [ 244 , 245 , 246 , 247 ]. Second, Aβ may also be generated inside autophagosomes because both APP (amyloid beta precursor protein) and PSEN1, an enzyme involved in the cleavage of APP to form Aβ, are found within the autophagosome [ 235 ].…”

The Impact of Electroacupuncture Early Intervention on the Brain Lipidome in a Mouse Model of Post-traumatic Stress Disorder

“…Previous studies have mainly focused on the analgesic effect of EA (Zhang et al, 2014). However, multiple studies found that EA not only has neuromodulatory effects (Liu Z. et al, 2017;Chen et al, 2018), but can also ameliorate the symptoms of several neuropsychiatric diseases, such as the cognitive impairment in Alzheimer's and Parkinson's disease, and anxiety and depression in unmarried patients with polycystic ovarian syndrome (Li et al, 2019;Tamtaji et al, 2019;Wang et al, 2019;Zheng et al, 2021). Interestingly, a large number of studies reported that EA exerts neuroprotective effects by regulating the structure and function of the hippocampus and PFC (He et al, 2018;Chen et al, 2019;Marcus et al, 2020).…”