Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche

Cai, Weiwei; Liu, Wenzong; Zhang, Zhaojing; Feng, Kai; Ren, Ge; Pu, Chuanliang; Li, Jiaqi; Deng, Ye; Wang, Aijie

doi:10.1016/j.scitotenv.2019.01.131

Cited by 31 publications

(11 citation statements)

References 44 publications

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“…The preacclimation enabled the cultures to maintain the PD values which would be beneficial for a community adapting to the environment. However, the washout and enrichment in the CSTRs indicated that a specifically functional unit would be enhanced with decreasing PD, which commonly occurred in our previous studies ( Cai et al., 2016 , Cai et al., 2019 , Cai et al., 2020 ).…”

Section: Resultssupporting

confidence: 48%

Linkage of community composition and function over short response time in anaerobic digestion systems with food fermentation wastewater

et al. 2021

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Summary We investigated the short-term dynamics of microbial composition and function in bioreactors with inocula collected from full-scale and laboratory-based anaerobic digestion (AD) systems. The Bray-Curtis dissimilarity of both inocula was approximately 10% of the predicted Kyoto Encyclopedia of Genes and Genomes pathway and 40% of the taxonomic composition and yet resulted in a similar performance in methane production, implying that the variation of community composition may be decoupled from performance. However, the significant correlation of volatile fatty acids with taxonomic variation suggested that the pathways of AD could be different because of the varying genus. The predicted function of the significantly varying genus was mostly related to fermentation, which strengthened the conclusion that most microbial variation occurred within the fermentative species and led to alternative routes to result in similar methane production in methanogenic bioreactors. This finding sheds some light on the understanding of AD community regulation, which depends on the aims to recover intermediates or methane.

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Section: Resultssupporting

confidence: 48%

Linkage of community composition and function over short response time in anaerobic digestion systems with food fermentation wastewater

et al. 2021

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“…Moreover, the hydrogen molecule which is recovered on the cathode can also work as the electron transfer carrier for methanogens as well as other hydrogen scavengers (12)(13)(14). Presumably, hydrogen-mediated electron transfer could be essential to extend the biofilm growth of a mixed culture (15,16). Overall, hydrogen could be a crucial mediator for electron transfer due to its being easily consumed by bacteria or methanogens with metabolic versatility (17).…”

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confidence: 99%

Semiquantitative Detection of Hydrogen-Associated or Hydrogen-Free Electron Transfer within Methanogenic Biofilm of Microbial Electrosynthesis

Cai

Liu

Wang

et al. 2020

Appl Environ Microbiol

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Hydrogen-entangled electron transfer is verified as an important extracellular pathway of sharing reducing equivalents to regulate biofilm activities within the diversely anaerobic environment, especially in microbial electrosynthesis system. However, the lack of useful methods for in-situ hydrogen detection in cathodic biofilm, the role of hydrogen involved in electron transfer is still debatable. Herein, the cathodic biofilm was constructed in the CH4-produced microbial electrosynthesis reactors, in which the hydrogen evolution dynamic was analyzed to confirm the presence of hydrogen-associated electron transfer nearby the cathode within the micrometre scale. The fluorescent in-situ hybridization images indicated that a colocalized community of archaea and bacteria developed within the thick of biofilm 58.10 μm at cathode suggesting the hydrogen gradient detected by microsensor was consumed by the collaboration of bacteria and archaea. Coupling microsensor and cyclic voltammetry test further provided the semi-quantitative results of hydrogen-associated contribution to methane generation ( around 21.20% ± 1.57% having a potential of -0.5 V to 0.69 V). This finding provides deep insight into the mechanism of electron transfer in biofilm on conductive materials. IMPORTANCE: Electron transfer from the electrode to biofilm is of great interest to the fields of microbial electrochemical technology, bioremediation and methanogenesis. It has promising application potential to boost more value-added products or pollutant degradation. Importantly, the microbial ability to obtain electrons from electrode and utilizing it brings a new insight into the direct interspecies electron transfer during methanogenesis. Previous studies had verified the direct pathway of electron transfer from the electrode to the pure-culture bacterium, but it was rarely reported how the methanogenic biofilm of mixed cultures shares electrons by hydrogen-associated or hydrogen-free pathway. In the current study, a combination method of microsensor and cyclic voltammetry successfully semi-quantified the role of hydrogen in the electron transfer from the electrode to methanogenic biofilm.

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“…68 Notably, according to the literature, the CH 4 content always increases with a concurrent decrease in H 2 and CO 2 production. 52 For example, Cai et al 69 detected CH 4 that reached a steady production rate when the H 2 production rate and content started to decline. Hu et al 70 also observed a sudden accumulation of CH 4 when the H 2 production rate decreased by half.…”

Section: Mechanisms Of Methane Production In Mecsmentioning

confidence: 99%

Suppressing Methane Production to Boost High-Purity Hydrogen Production in Microbial Electrolysis Cells

Tang

et al. 2022

Environ. Sci. Technol.

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Hydrogen gas (H2) is an attractive fuel carrier due to its high specific enthalpy; moreover, it is a clean source of energy because in the combustion reaction with oxygen (O2) it produces water as the only byproduct. The microbial electrolysis cell (MEC) is a promising technology for producing H2 from simple or complex organics present in wastewater and solid wastes. Methanogens and non-archaeal methane (CH4)-producing microorganisms (NAMPMs) often grow in the MECs and lead to rapid conversion of produced H2 to CH4. Moreover, non-archaeal methane production (NAMP) catalyzed by nitrogenase of photosynthetic bacteria was always overlooked. Thus, suppression of CH4 production is required to enhance H2 yield and production rate. This review comprehensively addresses the principles and current state-of-the-art technologies for suppressing methanogenesis and NAMP in MECs. Noteworthy, specific strategies aimed at the inhibition of methanogenic enzymes and nitrogenase could be a more direct approach than physical and chemical strategies for repressing the growth of methanogenic archaea. In-depth studies on the multiomics of CH4 metabolism can possibly provide insights into sustainable and efficient approaches for suppressing metabolic pathways of methanogenesis and NAMP. The main objective of this review is to highlight key concepts, directions, and challenges related to boosting H2 generation by suppressing CH4 production in MECs. Finally, perspectives are briefly outlined to guide and advance the future direction of MECs for production of high-purity H2 based on genetic and metabolic engineering and on the interspecific interactions.

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Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche

Cited by 31 publications

References 44 publications

Linkage of community composition and function over short response time in anaerobic digestion systems with food fermentation wastewater

Linkage of community composition and function over short response time in anaerobic digestion systems with food fermentation wastewater

Semiquantitative Detection of Hydrogen-Associated or Hydrogen-Free Electron Transfer within Methanogenic Biofilm of Microbial Electrosynthesis

Suppressing Methane Production to Boost High-Purity Hydrogen Production in Microbial Electrolysis Cells

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