Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats

Ding, Ying; Yan, Qing; Ruan, Jing Wen; Zhang, Yan Qing; Li, Wen Jie; Zhang, Yu Jiao; Li, Yan; Dong, Hongxin; Zeng, Yuan

doi:10.1186/1471-2202-10-35

Cited by 86 publications

(116 citation statements)

References 69 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…However, more work is required before these new mobilization regimes can be introduced into the clinic. Previous studies have reported that EA could promote the survival and homing of transplanted bone marrow MSCs [22]. Our study indicated that EA was a sensitive stimulus for MSC mobilization.…”

Section: Discussionsupporting

confidence: 47%

“…In addition, Chen and colleagues reported that electro-acupuncture (EA) improved survival and migration of transplanted neural stem cells in injured rat spinal cord [21]. Another study further showed that the survival and differentiation of transplanted MSCs were promoted by EA treatment [22]. We therefore hypothesized that acupuncture or EA might be an effective stimulus to induce the release of endogenous MSCs into the blood.…”

Section: Electro-acupuncture Promotes Endogenous Multipotential Mesenmentioning

confidence: 99%

“…EA on GV acupoints has been used widely in the clinic. Increasing evidence suggests that GV-EA promotes migration and differentiation of endogenous neurogenic stem cells [21,22,24]. Hence, we chose Jizhong (GV6) and Mingmen (GV4), one pair of "Governor Vessel" acupoints, to investigate the MSC-mobilizing effect of EA.…”

Section: Electro-acupuncture Promotes Endogenous Multipotential Mesenmentioning

confidence: 99%

See 2 more Smart Citations

Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood

Liu

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Mobilization of endogenous stem cells is an appealing strategy for cell therapy However, there is little evidence for reproducible, effective methods of mesenchymal stem cell (MSC) mobilization. In the present study, we investigated the mobilizing effect of electro-acupuncture (EA) on endogenous MSCs. Methods: Normal adult rats were randomly divided into six groups, namely, EA for 14 days (EA14d), sham EA14d, EA21d, sham EA21d and matched control groups. MSC mobilization efficiency was determined by colony-forming unit fibroblast (CFU-F) assays. Mobilized peripheral blood (PB)-derived MSCs were identified by immunophenotype and multi-lineage differentiation potential. Results: CFU-F frequency was significantly increased in the PB of EA14d rats compared with the sham EA and control groups. Moreover, the number of CFU-Fs was increased further in the EA21d group. MSCs derived from EA-mobilized PB were positive for CD90 and CD44, but negative for CD45. Additionally, these cells could differentiate into adipocytes, osteoblasts, chondrocytes and neural-like cells in vitro. Finally, stromal cell-derived factor-1α (SDF-1α) was increased in the PB of rats subjected to EA, and the migration of MSCs was improved in response to SDF-1α. Conclusions: MSCs with multi-lineage differentiation potential can be mobilized by EA. Our data provide a promising strategy for MSC mobilization.

show abstract

Section: Discussionsupporting

confidence: 47%

Section: Electro-acupuncture Promotes Endogenous Multipotential Mesenmentioning

confidence: 99%

Section: Electro-acupuncture Promotes Endogenous Multipotential Mesenmentioning

confidence: 99%

See 1 more Smart Citation

Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood

Liu

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…One of the pathological outcomes of CSCI is demyelination caused by myelin-forming oligodendrocyte death through necrosis and/or apoptosis [2,3]. Electroacupuncture (EA), which originated in ancient China, has been used in wide scope, particularly in neurological diseases, like CNS damage, autonomic nervous system dysfunction, and demyelinating diseases [4][5][6]. However, the therapeutic mechanism remains unclear in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Electroacupuncture on Neural Myelin Sheaths is Mediated via Promotion of Oligodendrocyte Proliferation and Inhibition of Oligodendrocyte Death After Compressed Spinal Cord Injury

et al. 2014

View full text Add to dashboard Cite

Electroacupuncture (EA) has been used worldwide to treat demyelinating diseases, but its therapeutic mechanism is poorly understood. In this study, a custom-designed model of compressed spinal cord injury (CSCI) was used to induce demyelination. Zusanli (ST36) and Taixi (KI3) acupoints of adult rats were stimulated by EA to demonstrate its protective effect. At 14 days after EA, both locomotor skills and ultrastructural features of myelin sheath were significantly improved. Phenotypes of proliferating cells were identified by double immunolabeling of 5-ethynyl-2'-deoxyuridine with antibodies to cell markers: NG2 [oligodendrocyte precursor cell (OPC) marker], 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase) (oligodendrocyte marker), and glial fibrillary acidic protein (GFAP) (astrocyte marker). EA enhanced the proliferation of OPCs and CNPase, as well as the differentiation of OPCs by promoting Olig2 (the basic helix-loop-helix protein) and attenuating Id2 (the inhibitor of DNA binding 2). EA could also improve myelin basic protein (MBP) and protect existing oligodendrocytes from apoptosis by inhibiting caspase-12 (a representative of endoplasmic reticulum stress) and cytochrome c (an apoptotic factor and hallmark of mitochondria). Therefore, our results indicate that the protective effect of EA on neural myelin sheaths is mediated via promotion of oligodendrocyte proliferation and inhibition of oligodendrocyte death after CSCI.

show abstract

“…Electrical stimulation can promote the proliferation and differentiation of endogenous neural stem cells and has been reported to enhance the reparative ability of nervous tissue [4,5]. Acusector and electric field treatments have been used for spinal cord injuries in both experimental animals and clinical practice [6][7][8][9]. Jiaji, also termed "Xieji" or "Xiaji," refers to acupuncture points (acupoints) located on either side of the vertebral column.…”

mentioning

confidence: 99%

Effect of Jiaji electroacupuncture in transected rat spinal cord

et al. 2011

View full text Add to dashboard Cite

We evaluated the effect of Jiaji electroacupuncture on cell proliferation and the expression of markers of endogenous neural stem cell activation after complete spinal cord transection. Female Wistar rats were assigned to 4 groups (n = 24 each): a sham-operated group, a control group, a Jiaji electroacupuncture group, and a Jiaji electroacupuncture preconditioning group. Motor function was significantly improved in the acupuncture groups compared to the control group at 7 and 14 d. Numbers of bromodeoxyuridine (BrdU)-, nestin-, and glial fibrillary acidic protein (GFAP)-positive cells were significantly greater in the acupuncture groups than in the controls at each time point. Expression of nestin and GFAP mRNA was significantly higher in the acupuncture groups than in the controls at each time point. Thus, Jiaji electroacupuncture and preconditioning may promote the proliferation of endogenous neural stem cells after spinal cord transection.

show abstract

Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats

Cited by 86 publications

References 69 publications

Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood

Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood

Protective Effect of Electroacupuncture on Neural Myelin Sheaths is Mediated via Promotion of Oligodendrocyte Proliferation and Inhibition of Oligodendrocyte Death After Compressed Spinal Cord Injury

Effect of Jiaji electroacupuncture in transected rat spinal cord

Contact Info

Product

Resources

About