Electricity Generation by <i>Geobacter sulfurreducens</i> Attached to Gold Electrodes

Richter, Hanno; McCarthy, Kevin; Nevin, Kelly P.; Johnson, Jessica P.; Rotello, Vincent M.; Lovley, Derek R.

doi:10.1021/la703469y

Cited by 253 publications

(165 citation statements)

References 32 publications

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“…The electron donation rate onto the anode was most similar to that of solid iron reduction, with much-higher rates obtained for soluble electron acceptors (fumarate and soluble iron). These results suggest that the electron transfer rate to the anode is constrained in a manner similar to that of solid iron reduction, consistent with previous reports (3,37).…”

Section: Resultssupporting

confidence: 81%

“…However, the electron flux to the anode was considerably lower than that estimated from oxygen reduction on the cathode in the air-cathode MFC. This phenomenon suggests that the electron transport system to the extracellular solid electron acceptor is the rate-limiting step for the G. sulfurreducens strain PCA, as it is to solid Fe(III) oxide (3,37,40).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Electrode Reduction Activities of Geobacter sulfurreducens and an Enriched Consortium in an Air-Cathode Microbial Fuel Cell

Ishii

Watanabe

Yabuki

et al. 2008

Appl Environ Microbiol

194

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An electricity-generating bacterium, Geobacter sulfurreducens PCA, was inoculated into a single-chamber, air-cathode microbial fuel cell (MFC) in order to determine the maximum electron transfer rate from bacteria to the anode. To create anodic reaction-limiting conditions, where electron transfer from bacteria to the anode is the rate-limiting step, anodes with electrogenic biofilms were reduced in size and tests were conducted using anodes of six different sizes. The smallest anode (7 cm 2 , or 1.5 times larger than the cathode) achieved an anodic reaction-limiting condition as a result of a limited mass of bacteria on the electrode. Under these conditions, the limiting current density reached a maximum of 1,530 mA/m 2 , and power density reached a maximum of 461 mW/m 2 . Per-biomass efficiency of the electron transfer rate was constant at 32 fmol cell ؊1 day ؊1 (178 mol g of protein ؊1 min ؊1 ), a rate comparable to that with solid iron as the electron acceptor but lower than rates achieved with fumarate or soluble iron. In comparison, an enriched electricity-generating consortium reached 374 mol g of protein ؊1 min ؊1 under the same conditions, suggesting that the consortium had a much greater capacity for electrode reduction. These results demonstrate that per-biomass electrode reduction rates (calculated by current density and biomass density on the anode) can be used to help make better comparisons of electrogenic activity in MFCs.Microbial fuel cells (MFCs) are devices that exploit microorganisms as "biocatalysts" of generating electric power from organic matter. MFC systems are being researched as a method of recovering energy from waste as electrical power (10,23,24,35) and generating power from aquatic sediments on the bottom of the ocean (25, 42) or from rice paddy soil (13,14). Recent technical improvements of MFC system architecture have increased power densities from Ͻ0.1 mW/m 2 to Ͼ2,400 mW/m 2 (normalized by the anode surface area) during the past several years in systems lacking exogenous electron shuttles (22,24). However, continued improvements are still needed for improved power densities, reduced costs for materials, and the development of large-scale devices (8).The two common ways of expressing MFC performance for power generation are power normalized to the projected surface area of an electrode (power density; mW/m 2 ) and power per unit of reactor volume (power output; W/m 3 ) (35). Many studies of MFCs have used power density based on the assumption that the biocatalytic activity of the anode limits power production (16,24,35,39). However, variations in the reactor volume (2, 38), composition of the proton-exchange membrane (17), catholyte reactions (32, 34), substrates (21), and anode materials (5, 33) often make it difficult to know which factors actually limit power production. There are various potential losses that can limit power output, such as microbial electron transfer to the anode, solution resistance, membrane resistance, and reduction reaction on the cathode (16, 35). The...

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Section: Resultssupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Comparison of Electrode Reduction Activities of Geobacter sulfurreducens and an Enriched Consortium in an Air-Cathode Microbial Fuel Cell

Ishii

Watanabe

Yabuki

et al. 2008

Appl Environ Microbiol

194

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“…Previously, it was found that bacteria of the genus Geobacter can perform the transfer of electrons to the graphite electrodes [44]. In the work [45] was demonstrated that Geobacter sulfurreducens can efficiently transfer electrons not only on graphite electrodes, but also when using as an anode of a gold electrode.…”

Section: Development Of the Bioanode Of A Fuel Element On The Basis Omentioning

confidence: 99%

The biofuel elements on the basis of the nanocarbon materials

Алферов¹,

Василов²,

Губин³

et al. 2014

RENSIT

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“…Regarding electrochemically active bacteria, species of genus Bacillus (Nimje et al 2009), Enterobacter (Rezaei et al 2009), Geobacter (Richter et al 2008), Proteus (Chen et al 1999), Shewanella (Watson and Logan 2010) have been explored to present practicability for bioelectricity generation. However, compared to pure microbial culture MFCs, mixed culture still exhibited the most promising stable power generation for longterm operation (Hassan et al 2017;Ishii et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri

Hsueh

Chen

2017

Bioresour. Bioprocess.

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This review tended to decipher the expression of electron transfer capability (e.g., biofilm formation, electron shuttles, swarming motility, dye decolorization, bioelectricity generation) to microbial fuel cells (MFCs). As mixed culture were known to perform better than pure microbial cultures for optimal expression of electrochemically stable activities to pollutant degradation and bioenergy recycling, Proteus hauseri isolated as a "keystone species" to maintain such ecologically stable potential for power generation in MFCs was characterized. P. hauseri expressed outstanding performance of electron transfer (ET)-associated characteristics [e.g., reductive decolorization (RD) and bioelectricity generation (BG)] for electrochemically steered bioremediation even though it is not a nanowire-generating bacterium. This review tended to uncover taxonomic classification, genetic or genomic characteristics, enzymatic functions, and bioelectricity-generating capabilities of Proteus spp. with perspectives for electrochemical practicability. As a matter of fact, using MFCs as a tool to evaluate ET capabilities, dye decolorizer(s) could clearly express excellent performance of simultaneous bioelectricity generation and reductive decolorization (SBG and RD) due to feedback catalysis of residual decolorized metabolites (DMs) as electron shuttles (ESs). Moreover, the presence of reduced intermediates of nitroaromatics or DMs as ESs could synergistically augment efficiency of reductive decolorization and power generation. With swarming mobility, P. hauseri could own significant biofilm-forming capability to sustain ecologically stable consortia for RD and BG. This mini-review evidently provided lost episodes of great significance about bioenergysteered applications in myriads of fields (e.g., biodegradation, biorefinery, and electro-fermentation). Keywords

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Electricity Generation by Geobacter sulfurreducens Attached to Gold Electrodes

Cited by 253 publications

References 32 publications

Comparison of Electrode Reduction Activities of Geobacter sulfurreducens and an Enriched Consortium in an Air-Cathode Microbial Fuel Cell

Comparison of Electrode Reduction Activities of Geobacter sulfurreducens and an Enriched Consortium in an Air-Cathode Microbial Fuel Cell

The biofuel elements on the basis of the nanocarbon materials

Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri

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