Electrically Addressable Cell Immobilization Using Phenylboronic Acid Diazonium Salts

Abstract

Holding cells: Individual gold electrodes can be activated by phenylboronic acid diazonium salts for the facile and reversible immobilization of eukaryotic cells (see scheme). This platform provides a simple method for on‐demand release of captured cells (yeast and macrophage) and can be used in single‐cell or array‐based studies.

Evaluation of boronate-containing polymer brushes and gels as substrates for carbohydrate-mediated adhesion and cultivation of animal cells

“…3) As follows from the image, the dots observed by AFM were outward extremities with typical heights up to 80 nm and average diameters within 200 nm. The NAAPBA-containing polymer brushes have been earlier characterized by time-of-flight secondary ion mass-spectrometry (ToF SIMS) that proved both the presence of boron and monomeric fragments of DMAAm in the polymer brush [13]. The B-Brush substrates were also characterized by the contact angles obtained in the absence and in the presence of 0.1 M fructose in the deposited droplets of aqueous buffer solutions.…”

“…Adhesion of animal cells on the affinity polymer brushes yielded irregular arrays formed by individual cells that could be, however, detached from the surface by means of elution with 0.1 M fructose. More recently, similar cell adhesion experiments have been successfully performed on gold electrodes chemically modified by diazonium salts of p-aminophenylboronic acid [13], a low molecular weight affinity ligand. The cell adhesion affinity molecules like RGD-type peptides are commonly immobilized on solid surfaces via polymeric spacers such as polyethylene glycol (PEO), polylysine or thermoresponsive poly-N-isopropylacrylamide [2].…”

Fabrication of biomembrane-like films on carbon electrodes using alkanethiol and diazonium salt and their application for direct electrochemistry of myoglobin

“…The diazonium-modified electrodes can withstand long-term storage in air, sonication, and repeated redox cycling over a quite wide electrochemical window (Delamar et al, 1992). This modification method is a very versatile and simple way to graft various functional groups for diverse applications (March et al, 2010;Polsky et al, 2008). To the best of our knowledge, this modification method has never been used for fabricating supported biomembrane-like films.…”

Doping Polypyrrole Films with 4-N-Pentylphenylboronic Acid to Enhance Affinity towards Bacteria and Dopamine

“…Surfaces functionalised with boronic group have been already reported for the rapid capture of bacteria, yeast and human cells on surfaces [55–57]. Polsky et al .…”

“…Polsky et al . [57] developed, via electrochemical grafting of diazomium-modified boronic-acid containing molecules, a functional gold electrode for the on-demand release of previously captured yeast and mammalian macrophage cells. Kuralay et al .…”