Monitoring
H2O2 has profound meaning for
food safety and life health. Generally, non-enzymatic H2O2 biosensors based on nanomaterials are subject to tedious
preparation and short working life. In this study, a facile H2O2 biosensor was proposed for simultaneously realizing
rapidity, simplicity, sensitivity, and selectivity. The in
situ electro-polymerized polypyrrole functionalized with
electrodeposited platinum-palladium nanoparticles (PPy-PtPd) was used
as the H2O2 sensing element. The Pt7Pd4 bimetallic nanoparticles may bind PPy through an NH–metal
interaction and endow glassy carbon electrodes robust electrocatalytic
performance to H2O2. By parameter optimization,
this H2O2 biosensor showed favorable electrochemical
performance toward H2O2 reduction with a wide
detection range of 2.5–8000 μM, high sensitivity (1360.83
μA·mM–1·cm–2),
favorable anti-interference property, reproductivity, and outstanding
endurance (> 60 days at room temperature). Importantly, the proposed
biosensor worked successfully for the determination of H2O2 in three kinds of biological samples. The electrochemical
biosensor using nanocomposites from electrochemistry provides a simple-make
and easy-use strategy for biosensor design. In the future, PPy-PtPd
NPs can be easily integrated onto high-end devices including microelectrodes
and implantable and wearable biosensors for detecting H2O2
in vivo and in vitro.