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Besse, Pascale; Taylor, G O; Carroll, Bernard J.; Berding, N.; Burner, David; McIntyre, C. Lynne

doi:10.1023/a:1003436403678

Cited by 66 publications

(9 citation statements)

References 32 publications

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“…SSRs can give high levels of genetic information and are therefore useful in estimates of genetic variability and in genomic analysis across species. SSRs are more robust [12], variable, and informative than markers based on RFLPs, RAPDs, and AFLPs [6,8].…”

Section: Application Of Ssr Markersmentioning

confidence: 99%

“…Molecular marker technologies have been used in genetic diversity studies, molecular assisted selection (MAS), paternity analysis, quantitative trait loci (QTL) mapping, cultivar identification, phylogenetic relationships, and genetic mapping. Various molecular markers are being developed and used in sugarcane genetic studies, including simple sequence repeats (SSRs) [5], inter simple sequence repeat (ISSRs), restriction fragment length polymorphisms (RFLPs) [6], random amplification of polymorphic DNAs (RAPDs) [7], amplified fragment length polymorphisms (AFLPs) [8], and single nucleotide polymorphisms (SNPs) [9]. Among these, SSRs, also known as microsatellites, are very advantageous for a variety of applications in sugarcane genetics research and breeding, because they are multi-allele, co-dominant, highly informative, occur with high relative abundance and good coverage across the genome, and are experimentally reproducible [5,10,11].…”

Section: Introductionmentioning

confidence: 99%

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Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

et al. 2018

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Recently-developed molecular markers are becoming powerful tools, with applications in crop genetics and improvement. Microsatellites, or simple sequence repeats (SSRs), are widely used in genetic fingerprinting, kinship analysis, and population genetics, because of the advantages of high variability from co-dominant and multi-allelic polymorphisms, and accurate and rapid detection. However, more recent evidence suggests they may play an important role in genome evolution and provide hotspots of recombination. This review describes the development of SSR markers through different techniques, and the detection of SSR markers and applications for sugarcane genetic research and breeding, such as cultivar identification, genetic diversity, genome mapping, quantitative trait loci (QTL) analysis, paternity analysis, cross-species transferability, segregation analysis, phylogenetic relationships, and identification of wild cross hybrids. We also discuss the advantages and disadvantages of SSR markers and highlight some future perspectives.

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Section: Application Of Ssr Markersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

et al. 2018

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“…AFLPs have been used to assess genetic diversity in germplasm collections of sugarcane and close relatives [49-52], and to build linkage maps [19,53]. In light of these studies, AFLPs have been informative in generating a substantial amount of unambiguous polymorphic markers.…”

Section: Discussionmentioning

confidence: 99%

A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers

et al. 2012

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Background: The development of sugarcane as a sustainable crop has unlimited applications. The crop is one of the most economically viable for renewable energy production, and CO 2 balance. Linkage maps are valuable tools for understanding genetic and genomic organization, particularly in sugarcane due to its complex polyploid genome of multispecific origins. The overall objective of our study was to construct a novel sugarcane linkage map, compiling AFLP and EST-SSR markers, and to generate data on the distribution of markers anchored to sequences of scIvana_1, a complete sugarcane transposable element, and member of the Copia superfamily. Results: The mapping population parents ('IAC66-6' and 'TUC71-7') contributed equally to polymorphisms, independent of marker type, and generated markers that were distributed into nearly the same number of cosegregation groups (or CGs). Bi-parentally inherited alleles provided the integration of 19 CGs. The marker number per CG ranged from two to 39. The total map length was 4,843.19 cM, with a marker density of 8.87 cM. Markers were assembled into 92 CGs that ranged in length from 1.14 to 404.72 cM, with an estimated average length of 52.64 cM. The greatest distance between two adjacent markers was 48.25 cM. The scIvana_1-based markers (56) were positioned on 21 CGs, but were not regularly distributed. Interestingly, the distance between adjacent scIvana_1-based markers was less than 5 cM, and was observed on five CGs, suggesting a clustered organization.

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“…Several studies to determine the genetic diversity within and between members of the Saccharum complex have been carried out using molecular systems such as AFLPs (Besse et al, 1998), RAPDs , isozymes (Glaszmann et al, 1989), RFLPs (Lu et al, 1994;Besse et al, 1997) and 5S rRNA intergenic spacer sequences (Pan et al, 2000). All these properties make EST-SSRs more popular among the existing markers for development of molecular maps or QTL analysis.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity among Sugarcane Cultivars (<i>Saccharum officinarum</i> L.) using Simple Sequence Repeats Markers

Pandey

Mishra

et al. 2011

OnLine Journal of Biological Sciences

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Problem statement:Increasing sugar productivity is the main concern of sugarcane (Saccharum officinarum L.) breeding programs. The complexity and size of the sugarcane genome is a major limitation in its genetic improvement. Characterization of sugarcane provides essential information of genetic diversity for breeders utilize for crop improvement. Approach and Results: The objective of this study was to evaluate the microsatellite markers (SSR) with 17 sugarcane accessions to access the genetic diversity and inter relationships in sugarcane. Genetic distances for SSR data (polymorphic fragments) were determined and relationships between samples were portrayed graphically in the form of a dendrogram and similarities are ranging from 36% to 100% were observed. The lowest genetic similarity of 36% was seen between sample 9 and 11 with other samples. These two genotypes differed from each other with only 68% similarity. Conclusion: Results illustrate that SSR markers could be useful for structuring the genetic diversity of collections according to geographical origin and ploidy level, assessment or formation of a core collection and especially construction of a genetic map.

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Cited by 66 publications

References 32 publications

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers

Assessment of Genetic Diversity among Sugarcane Cultivars (<i>Saccharum officinarum</i> L.) using Simple Sequence Repeats Markers

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