Elateriospermum tapos Yogurt Supplement in Maternal Obese Dams during Pregnancy Modulates the Body Composition of F1 Generation

Abstract

Maternal obesity is a key predictor of childhood obesity and a determining factor for a child’s body composition. Thus, any form of maternal nutrition during the gestational period plays a vital role in influencing the growth of the fetus. Elateriospermum tapos (E. tapos) yogurt has been found to comprise many bioactive compounds such as tannins, saponins, α-linolenic acid, and 5′-methoxy-bilobate with apocynoside I that could cross the placenta and exhibit an anti-obesity effect. As such, this study aimed to investigate the role of maternal E. tapos yogurt supplementation on offspring body composition. In this study, 48 female Sprague Dawley (SD) rats were induced with obesity using a high-fat diet (HFD) and were allowed to breed. Upon confirmation of pregnancy, treatment was initiated with E. tapos yogurt on the obese dams up to postnatal day 21. The weaning offspring were then designated into six groups according to their dam’s group (n = 8) as follows; normal food and saline (NS), HFD and saline (HS), HFD and yogurt (HY), HFD and 5 mg/kg of E. tapos yogurt (HYT5), HFD and 50 mg/kg of E. tapos yogurt (HYT50), and HFD and 500 mg/kg of E. tapos yogurt (HYT500). The body weight of the offspring was accessed every 3 days up to PND 21. All the offspring were euthanized on PND 21 for tissue harvesting and blood sample collection. The results showed that both male and female offspring of obese dams treated with E. tapos yogurt showed growth patterns similar to NS and reduced levels of triglycerides (TG), cholesterol, LDL, non-HDL, and leptin. Liver enzymes such as ALT, ALP, AST, GGT, and globulin, and renal markers such as sodium, potassium, chloride, urea, and creatinine levels significantly reduced (p < 0.05) in the offspring of E. tapos yogurt-treated obese dams with the normal histological architecture of the liver, kidney, colon, RpWAT, and visceral tissue that is comparable to NS. In toto, E. tapos yogurt supplementation of obese dams exerted an anti-obesity effect by preventing intergenerational obesity by reversing HFD-induced damage in the fat tissue of the offspring.

Remodulation Effect of Elateriospermum tapos Yoghurt on Metabolic Profile of Maternal Obesity Induced Cognitive Dysfunction and Anxiety-like Behavior in Female Offspring—An In Vivo Trial on Sprague Dawley Rats

“…Dysregulation of glucose homeostasis in obesegenic exposed offspring may appear as insulin resistance during their adolescence [ 40 , 41 ]. Thus, the high BMI and lee index of the offspring after weaning mimics the maternal obesity inherited obesogenic offspring rat model [ 42 , 43 ]. Consistent with this previous study, the BMI and lee index of female offspring of HFD supplemented dams is significantly heavier compared to the NS group.…”

Elateriospermum tapos Yoghurt as a Therapeutic Intervention for Obesity-Associated Cognitive Impairments and Anxiety-like Behaviour in a High Fat Diet Maternal Obese Rat Model

“…Next, a starter culture consisting of live bacterial strains was added to the boiled milk, and the mixture was placed in a yoghurt maker (Pensonic PYM-700) and incubated for 7 to 8 h. The resulting yoghurt was then chilled in the refrigerator overnight at a temperature of 4 °C. The next day, E. tapos powder was added [ 20 ] and incorporated into the yoghurt at a ratio of 2 g per 100 mL [ 21 ].…”

Association of vitamin B1 intake with geriatric cognitive function: An analysis of the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2014

“…While pharmacological treatments for cognitive decline are limited, lifestyle modifications and dietary interventions have proven highly effective in slowing down its progression [ 9 ]. Risk factors associated with cognitive impairment are being increasingly reported, emphasizing the significance of lifestyle and dietary factors associated with cognitive function [ [10] , [11] , [12] , [13] , [14] , [15] , [16] ].…”