Elastic modulus of low-density lipoprotein as potential indicator of its oxidation

Takeda, Seiji; Subagyo, Agus; Hui, Shu‐Ping; Shrestha, Rojeet; Sueoka, Kazuhisa; Chiba, Hitoshi

doi:10.1177/0004563215584958

Cited by 5 publications

(6 citation statements)

References 33 publications

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“…This result is in good agreement with many pervious results as pointed out in the Introduction [10,27,28]. The result indicates that the size of LDL particles is not only affected by the oxidation [10] but also due to its adsorption and compression at the air/water interface; however, the compression elasticity of the LDL film is also likely to be affected by these external stimuli [13,25,26]. Johs et al [27] investigated a 3D model of apoB-100 structure using spectroscopic and small-angle neutron scattering in combination with advanced shape reconstruction modeling to generate a three-dimensional model of lipid-free apoB-100 protein.…”

Section: Pressure-area Isotherms Of Ldl/elasticity Of Ldl Particlessupporting

confidence: 93%

“…This is done by assuming that all of the LDL are spherical-like particles tightly arranged on the air/water interface with an estimated mean diameter per particle of 20 nm. This result is in good agreement with many pervious results as pointed out in the Introduction [10,27,28]. The result indicates that the size of LDL particles is not only affected by the oxidation [10] but also due to its adsorption and compression at the air/water interface; however, the compression elasticity of the LDL film is also likely to be affected by these external stimuli [13,25,26].…”

Section: Pressure-area Isotherms Of Ldl/elasticity Of Ldl Particlessupporting

confidence: 91%

“…The latter process progresses further to atherosclerotic plaques in the vascular membrane arterial wall [8,9]. This in turn affects the apoB-100 secondary structure by modifying the inner artery layer elastic properties [10].…”

Section: Introductionmentioning

confidence: 99%

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A correlation between secondary structure and rheological properties of low-density lipoproteins at air/water interfaces

Khattari

2017

J Biol Phys

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The secondary structure of apolipoprotein B-100 is studied within the bulk phase and at the air/water interface. In these "in viro" experiments, infrared reflection absorption spectroscopy (IRRAS) study was performed at the air/water interface while circular dichroism (CD) was conducted in the bulk phase. In the bulk phase, the conformational structure containing a significant amount of β-structure, whereas varying amount of α-helix, unordered structures, and β-sheet were observed at the air/water interface depending on the low-density lipoprotein (LDL) film interfacial pressure. The present IRRAS results demonstrate the importance of interfacial pressure-induced structural conformations on the apoB-100. A correlation between the secondary structure of the apoB-100 protein and the monomolecular film elasticity at the air/water interface was also established. The orientation of apoB-100 with respect to the LDL film-normal was found to depend on the interfacial pressure exhibited by the monomolecular film. These results may shed light on LDL's pivotal role in the progression of atherosclerotic coronary artery disease as demonstrated previously by clinical trials.

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Section: Pressure-area Isotherms Of Ldl/elasticity Of Ldl Particlessupporting

confidence: 93%

Section: Pressure-area Isotherms Of Ldl/elasticity Of Ldl Particlessupporting

confidence: 91%

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A correlation between secondary structure and rheological properties of low-density lipoproteins at air/water interfaces

Khattari

2017

J Biol Phys

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“…Takeda et al [13] reported that the elastic modulus (or Young's modulus) of LDL particles ranges between 0.1 and 2 MPa with an average of ~ 1 MPa and that oxLDL particles have smaller elastic moduli [14]. Their average elastic moduli are more than 20-fold lower than the data in our current study (20.1 ± 1.8 MPa and 16.7 ± 2.6 MPa for LDL and oxLDL, respectively).…”

Section: Effect Of Oxidation On the Stiffness Of Ldl Particlescontrasting

confidence: 81%

“…In previous studies on the biomechanical properties of LDL/oxLDL [5,13,14], a monolayer or even multilayer of LDL/oxLDL particles was detected by AFM. Another important improvement in the current study was that each of dispersedly distributed LDL/oxLDL particles in a topographical image could find a corresponding particlelike patch at the same location in a biomechanical image (Figs.…”

Section: Technical Improvements In Comparison With Previous Afm Studimentioning

confidence: 99%

Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein

Wang

Luo

et al. 2020

J Nanobiotechnol

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Background Low-density lipoprotein (LDL) is an important plasma lipoprotein transporting lipids to peripheral tissues/cells. The oxidation of LDL plays critical roles in atherogenesis and its oxidized form (oxLDL) is an important risk factor of atherosclerosis. The biomechanical properties of LDL/oxLDL are closely correlated with the disease. To date, however, the oxidation-induced changes in size and biomechanical properties (stiffness and stickiness) of LDL particles are less investigated. Methods In this study, copper-induced LDL oxidation was confirmed by detecting electrophoretic mobility, malondialdehyde production, and conjugated diene formation. Then, the topographical and biomechanical mappings of LDL particles before/after and during oxidation were performed by using atomic force microscopy (AFM) and the size and biomechanical forces of particles were measured and quantitatively analyzed. Results Oxidation induced a significant decrease in size and stiffness (Young’s modulus) but a significant increase in stickiness (adhesion force) of LDL particles. The smaller, softer, and stickier characteristics of oxidized LDL (oxLDL) partially explains its pro-atherosclerotic role. Conclusions The data implies that LDL oxidation probably aggravates atherogenesis by changing the size and biomechanical properties of LDL particles. The data may provide important information for a better understanding of LDL/oxLDL and atherosclerosis.

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Effects of enzymes on elastic modulus of low-density lipoproteins were investigated using atomic force microscopy

Takeda

Sakurai

Hui

et al. 2018

Biochemical and Biophysical Research Communications

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Elastic modulus of low-density lipoprotein as potential indicator of its oxidation

Cited by 5 publications

References 33 publications

A correlation between secondary structure and rheological properties of low-density lipoproteins at air/water interfaces

A correlation between secondary structure and rheological properties of low-density lipoproteins at air/water interfaces

Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein

Effects of enzymes on elastic modulus of low-density lipoproteins were investigated using atomic force microscopy

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