Effects of enzymes on elastic modulus of low-density lipoproteins were investigated using atomic force microscopy

Takeda, Seiji; Sakurai, ﻿Toshihiro; Hui, Shu‐Ping; Fuda, Hirotoshi; Chiba, Hitoshi

doi:10.1016/j.bbrc.2018.04.211

Cited by 3 publications

(3 citation statements)

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“…Takeda et al [13] reported that the elastic modulus (or Young's modulus) of LDL particles ranges between 0.1 and 2 MPa with an average of ~ 1 MPa and that oxLDL particles have smaller elastic moduli [14]. Their average elastic moduli are more than 20-fold lower than the data in our current study (20.1 ± 1.8 MPa and 16.7 ± 2.6 MPa for LDL and oxLDL, respectively).…”

Section: Effect Of Oxidation On the Stiffness Of Ldl Particlescontrasting

confidence: 76%

“…Secondly, their elastic moduli were estimated from the region of the contact point to 6 nm of indentation, in another word, many values were excluded artificially. Finally, in all of the three previous studies including ours [5,13,14], an one-to-one relationship of individual particles between topographical mapping and force mapping could not be obtained. Therefore, we think that the Young's modulus data in the current study is more accurate.…”

Section: Effect Of Oxidation On the Stiffness Of Ldl Particlesmentioning

confidence: 72%

“…In previous studies on the biomechanical properties of LDL/oxLDL [5,13,14], a monolayer or even multilayer of LDL/oxLDL particles was detected by AFM. Another important improvement in the current study was that each of dispersedly distributed LDL/oxLDL particles in a topographical image could find a corresponding particlelike patch at the same location in a biomechanical image (Figs.…”

Section: Technical Improvements In Comparison With Previous Afm Studimentioning

confidence: 99%

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Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein

Wang

Luo

et al. 2020

J Nanobiotechnol

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Background Low-density lipoprotein (LDL) is an important plasma lipoprotein transporting lipids to peripheral tissues/cells. The oxidation of LDL plays critical roles in atherogenesis and its oxidized form (oxLDL) is an important risk factor of atherosclerosis. The biomechanical properties of LDL/oxLDL are closely correlated with the disease. To date, however, the oxidation-induced changes in size and biomechanical properties (stiffness and stickiness) of LDL particles are less investigated. Methods In this study, copper-induced LDL oxidation was confirmed by detecting electrophoretic mobility, malondialdehyde production, and conjugated diene formation. Then, the topographical and biomechanical mappings of LDL particles before/after and during oxidation were performed by using atomic force microscopy (AFM) and the size and biomechanical forces of particles were measured and quantitatively analyzed. Results Oxidation induced a significant decrease in size and stiffness (Young’s modulus) but a significant increase in stickiness (adhesion force) of LDL particles. The smaller, softer, and stickier characteristics of oxidized LDL (oxLDL) partially explains its pro-atherosclerotic role. Conclusions The data implies that LDL oxidation probably aggravates atherogenesis by changing the size and biomechanical properties of LDL particles. The data may provide important information for a better understanding of LDL/oxLDL and atherosclerosis.

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Section: Effect Of Oxidation On the Stiffness Of Ldl Particlescontrasting

confidence: 76%

Section: Effect Of Oxidation On the Stiffness Of Ldl Particlesmentioning

confidence: 72%