Elaboration of Urease Adsorption on Silicalite for Biosensor Creation

Abstract

A possibility of efficient urease adsorption on silicalite for the purpose of biosensor creation was investigated. The procedure of urease adsorption on silicalite is notable for such advantages as simple and fast performance and non‐use of toxic or auxiliary compounds. Optimal conditions for modifying transducer surfaces with silicalite and subsequent urease adsorption on these surfaces were selected. The working parameters of the created biosensor were optimized. The developed biosensor with adsorbed urease was characterized by good intra‐reproducibility (RSD – 4.5 %), improved inter‐reproducibility (RSD of urea determination is 9 %) and operational stability (less than 10 % loss of activity after 10 days). Besides, the developed method for enzyme adsorption on silicalite was compared with the traditional methods of urease immobilization in biosensorics. Working conditions of the produced biosensor (pH and ionic strength) were shown to be close to those of the biosensor based on urease immobilized in GA vapor. For these reasons, it was concluded that the method of enzyme adsorption on silicalite is well‐suited for biosensor standardization aimed at its further manufacture.

Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation

“…The described effect was also observed in the biosensors based on urease [17] and urease co-immobilized with arginase [18]. Another option of the use of nanoscale materials in the design of biosensors has been proposed in [19], namely silicalite as a carrier for the enzyme sorption. The biosensors obtained were characterized by a significantly higher signal reproducibility.…”

“…The main goal of this research was to develop a new amperometric biosensor with enhanced analytical characteristics by using a novel method of immobilization of the enzyme based on its adsorption on zeolites. This method was first proposed for conductometric biosensors [19]. In this work we presented our attempt to use the mentioned method and to upgrade it for the amperometric transducers.…”

“…At first, we fabricated a number of biosensors based on GOD immobilized by the method of enzyme adsorption on silicalite proposed in [19]. In details the immobilization technique is described in «Materials and Methods».…”

Disposable urea biosensor based on nanoporous ZnO film fabricated from omissible polymeric substrate

“…9 C, showed a maximum interference of 2.1% with the presence of ascorbic acid. The results illustrated the importance of the prepared biosensor (FTO/ZnO/Urs) in the selective detection of urea.Finally, based on the enzyme electrostatic immobilization strategy, the response characteristics of the FTO/ZnO/Urs biosensor with the other reported urea biosensors was compared and summarized inTable 2[45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63]. We are currently developing nanostructured ZnO based urea biosensing using impedimetric assessment.…”

Development of New Biosensor for Arginine Determination in Pharmaceuticals

“…Першою методикою, що порівнювались в роботі, була іммобілізація ферменту методом адсорбції на силікаліті [15]. Спочатку необхідно було сфор-мувати шар силікаліту, а далі зверху нанести шар ферментного гелю на основі уреази.…”