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Pitta‐Alvarez, Sandra I.; Spollansky, Tatiana C.; Giulietti, Ana María

doi:10.1023/a:1005638029034

Cited by 22 publications

(9 citation statements)

References 12 publications

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“…Elicitation of two tropane alkaloids, scopolamine and hyoscyamine, by AgNO 3 and CdCl 2 in hairy root cultures of Brugmansia candida (angel's trumpet) has been investigated [69]. Scopolamine is an alkaloid drug obtained from plants of the nightshade family (Solanaceae), chiefly from henbane ( Hyoscyamus niger ).…”

Section: Abiotic Elicitorsmentioning

confidence: 99%

“…Intensive research has been devoted to establishing the mechanism of elicitation in plants, but fungal systems have received less attention. Research on both cultures has focused mainly on the biotic and particularly carbohydrate elicitors, and the effects of abiotic elicitors on the overproduction of secondary metabolites in plants is poorly understood; elicitation is hypothesized to involve the key messenger Ca 2+ , factors affecting cell membrane integrity, inhibition/activation of intracellular pathways and changes in osmotic pressure acting as stress agents [13, 69]. The present review focuses on biotic elicitors (particularly carbohydrates).…”

Section: Mechanism Of Elicitationmentioning

confidence: 99%

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Elicitation of plants and microbial cell systems

Radman

Sáez

Bucke

et al. 2003

Biotech and App Biochem

276

122

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Plants show physiological and morphological responses to a range of physical and chemical factors known as 'elicitors'. These responses have been considered as defence reactions 'elicited' by the plants' biochemical factory to ensure their survival, persistence and competitiveness. Recently examples have been cited of elicitation in some fungal and bacterial cultures. Through a chronological survey, this Review considers examples of elicitors and elicitation and describes suggested mechanisms of elicitation in plants and microbial cell cultures. The majority of research in this field has been carried out on the plant systems using complex (undefined) biotic elicitors. Carbohydrates are the main class of compounds used as defined elicitors. This Review focuses on carbohydrates as compounds initiating a defence response in cell cultures. Physiological changes brought about on the plant and microbial cultures include expression of novel metabolites and overproduction of already known products. Recent reports confirming elicitation in microbial cultures are of potential importance, as the relative ease of fermentation and scale-up could open an opportunity for the introduction of useful novel metabolites as well as enhancement of commercially useful bioproducts. In this context, a sound knowledge of the elicitor molecules' structure-function relationships and mechanisms of elicitation is essential.

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Section: Abiotic Elicitorsmentioning

confidence: 99%

Section: Mechanism Of Elicitationmentioning

confidence: 99%

Elicitation of plants and microbial cell systems

Radman

Sáez

Bucke

et al. 2003

Biotech and App Biochem

276

122

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“…In HR cultures of B. candida instability in endogenous SA level significantly stimulates the release of tropane alkaloids. Further, flux in SA level is also found to be accountable for the stimulation of related biosynthetic gene expression in Scopolia parviflora HR cultures [55][56][57].…”

Section: Transcription Factors Involved In Elicitation and Activationmentioning

confidence: 83%

Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

Goel

Mehrotra

Kukreja

2011

Appl Biochem Biotechnol

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A wide range of external stress stimuli triggers a plant cell to undergo a complex network of reactions that ultimately lead to the synthesis and accumulation of secondary metabolites. These secondary metabolites help the plant to survive under stress challenge. The potential of biotic and abiotic elicitors for the induction and enhancement of secondary metabolite production in various culture systems including hairy root (HR) cultures is well-known. The elicitor-induced defense responses involves signal perception of elicitor by a cell surface receptor followed by its transduction involving some major cellular and molecular events including activation of major secondary message signaling pathways. This result in induction of gene expressions escorting to the synthesis of various proteins mainly associated with plant defense responses and secondary metabolite synthesis and accumulation. The review discusses the elicitor-induced various cellular and molecular events and correlates them with enhanced secondary metabolite synthesis in HR systems. Further, this review also concludes that combining elicitation with in-silico approaches enhances the usefulness of this practice in better understanding and identifying the rate-limiting steps of biosynthetic pathways existing in HRs which in turn can contribute towards better productivity by utilizing metabolic engineering aspects.

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“…13,14) Especially, there were many reports concerning induction by plant hormones, jasmonic acid, and methyl jasmonate (MeJA). In many cases, MeJA enhanced the production of pharmacologically important secondary metabolites such as defense compounds, phytoalexins, [15][16][17] and defence systems in plants.…”

mentioning

confidence: 99%

“…11,12) Elicitation of hairy roots on culture with heavy metal ions such as cupper ion, organic chemicals such as jasmonic acid and biological substances such as yeast extract was studied, and found to enhance defense processes in plants and the production of secondary metabolites such as phytoalexins. 13,14) Especially, there were many reports concerning induction by plant hormones, jasmonic acid, and methyl jasmonate (MeJA). In many cases, MeJA enhanced the production of pharmacologically important secondary metabolites such as defense compounds, phytoalexins, [15][16][17] and defence systems in plants.…”

mentioning

confidence: 99%

Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate

Kawauchi

Arima

Shirota

et al. 2010

CHEMICAL & PHARMACEUTICAL BULLETIN

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The gram-negative bacteria Agrobacterium rhizogenes is a plant pathogen. On infecting plants it induces adventitious roots, known as "hairy roots." Hairy roots have an excellent growth capacity in simple culture systems without requiring the addition of any exogenous plant hormones.1,2) Furthermore, hairy roots are known to produce secondary metabolites at a high yield compared to those of undifferentiated plant cell suspensions and their mother plants. So, hairy roots were expected to provide a culture system for the production of pharmacologically important natural products and the biotransformation of organic compounds into useful biologically active compounds. Thus, many medicinal plants have been transformed to hairy roots, and there have been many related reports on the efficient production of useful natural products 3-7) and biotransformation of organic substances into useful compounds.8-10) Furthermore, hairy roots were expected to become a simple and easy experimental system for the biosynthesis of secondary metabolites such as phytoalexins, and plant gene engineering.11,12) Elicitation of hairy roots on culture with heavy metal ions such as cupper ion, organic chemicals such as jasmonic acid and biological substances such as yeast extract was studied, and found to enhance defense processes in plants and the production of secondary metabolites such as phytoalexins. 13,14) Especially, there were many reports concerning induction by plant hormones, jasmonic acid, and methyl jasmonate (MeJA). In many cases, MeJA enhanced the production of pharmacologically important secondary metabolites such as defense compounds, phytoalexins, [15][16][17] and defence systems in plants. 18) We reported the production of a coumarin-type phytoalexin, umbelliferone, in Pharbitsis nil hairy roots.19) The P. nil hairy roots also showed substrate specific and strong reactivity on the glucosylation of low-molecular-weight phenolic compounds, and strong reactivity on the reduction of a formyl group on the aromatic ring to a hydroxymethyl group. 20,21) Solanaceae, such as Solanum, Hyoscyamus, and Atropa sp., were used as medicinal plants, yielding alkaloids such as nicotine and tropan alkaloids as the main constituents, but they produced sesquiterpene-type phytoalexins, such as lubimine 22) and solavetivone, 23) after treatment with biotic and abiotic elicitors. We reported the production of sesquiterpene-type phytoallexins in Hyoscyamus albus (Solanaceae) hairy roots treated with MeJA,24) and the efficient production of tropane alkaloids in H. niger hairy roots. 25) We also reported the identification of a strongly expressed gene in H. niger hairy roots, related to and enhancing lateral root formation in H. niger hairy roots. 26) We previously found that products from H. albus hairy roots treated with CuSO 4 , MeJA, and co-treatment of CuSO 4 -MeJA showed different production patterns in terms of the TLC profile. So, we attempted to produce sesquiterpene-type phytoallexins in H. albus hairy roots cell culture by co-treatment with...

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Untitled

Cited by 22 publications

References 12 publications

Elicitation of plants and microbial cell systems

Elicitation of plants and microbial cell systems

Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate

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