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Abstract

Hyperglycaemia reduces proliferation of bovine aortic endothelial cells in vitro. A similar effect in vivo may contribute to long-term complications of diabetes such as impaired wound-healing and retinopathy. We report the effect of increased glucose concentrations, glycated basic fibroblast growth factor (FGF-2) and bovine serum albumin-derived advanced glycation endproducts (BSA-AGE) on the proliferation of bovine aortic endothelial cells. Glucose (30 and 50 mmol/l) had an antiproliferative effect on endothelial cells. This effect may be mediated through reduced mitogenic activity of FGF-2. The glycation of FGF-2 with 250 mmol/l glucose-6-phosphate led to reduced mitogenic activity compared to native FGF-2. BSA-AGE at concentrations of 10, 50 and 250 microg/ml had an antiproliferative effect on cultured endothelial cells. Aminosalicylic acid at a concentration of 200 micromol/l proved to be more effective than equimolar concentrations of aminoguanidine in protecting endothelial cells against the antiproliferative effects of both high (30 mmol/l) glucose and 50 microg/ml BSA-AGE. FGF-2 glycated in the presence of 4 mmol/l aminosalicylic acid or aminoguanidine retained mitogenic activity compared to that glycated in their absence. Compounds like aminoguanidine and, in particular, aminosalicylic acid protect endothelial cells against glucose-mediated toxicity and may therefore have therapeutic potential.

Chemo-biological evaluation of antidiabetic activity of Mentha arvensis L. and its role in inhibition of advanced glycation end products

“…One therapeutic approach suggested to reduce postprandial hyperglycemia is by the inhibition of two key enzymes linked to type 2 diabetes mellitus, namely α-glucosidase and α-amylase, in the digestive organs [9]. Recently, it has been reported that compounds with combined antioxidant potential and antiglycation properties are effectively used to treat diabetes mellitus [8].…”

Anti-Advanced glycation end-product and free radical scavenging activity of plants from the yucatecan flora

“…[18] However, although numerous AGE inhibitors, including the well-known aminoguanidine, ameliorated diabetic complications in an animal model, they also caused severe side effects including gastrointestinal disturbance, anemia, and flu-like symptoms. [19] Natural products represent an important option for the discovery and development of new anti-AGE pharmaceuticals; these can include metabolites with antioxidant activity since oxidative stress is involved and accelerates the formation of AGEs.…”

Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil

“…The FRAP assay measures the ability of antioxidants to reduce ferric ion in the complex [Fe(III)(TPTZ) 2 ] 3+ to [Fe(II)(TPTZ) 2 ] 2+ through electron transfer and cannot detect compounds that act by HAT (Duraisamy et al. 2003 ; Prior et al. 2005 ; Craft et al.…”

“…Compounds offering both properties have been reported to show greater efficacy for treating diabetes mellitus versus compounds targeting an individual pathway (Duraisamy et al. 2003 ). As such, on-going screening of natural compounds that offer combined antioxidant and anti-glycation properties with relatively low toxicity are promising candidates for the development of functional additives aimed at reducing protein glycation for the treatment and management of oxidative stress-related diseases, diabetic complications and other AGE-associated diseases (Elosta et al.…”