Ekstraksi, Hidrolisis dan Partisi Metabolit Sekunder dari Mikroalga Chlorella sp.

Fasya, Ahmad Ghanaim; Dinasti, Anike Riska; Shofiyah, Muharromatus; Rahmawati, Laili Maghfiroh; Millati, Nuria; Safitri, Dany Aulia; Handoko, Singgih; Hanapi, Ahmad; Ningsih, Rachmawati

doi:10.18860/al.v5i1.3686

Cited by 5 publications

(4 citation statements)

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“…Table 1 shows that the extraction process produces extracts of Chlorella sp., Spirulina sp., and Sargassum sp. This result is lower than research conducted by Ahmad Ganain [14], which obtained the yield of Chlorella sp. extract at 80.95%.…”

Section: Extraction Processcontrasting

confidence: 78%

Evaluation and Comparison Anti-aging Facial Serum from Algae Extract

Permadi,

Aziz,

Ramadani

et al. 2024

CHEMICA: Jurnal Teknik Kimia

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Interest in cosmetics now no longer looks at gender and class. This is due to the development of the cosmetics industry, which is no longer segmented and targets all age ranges, young and old, even children, and no longer looks at gender [1]. However, along with the increasing number of cosmetics consumption in Indonesia, various cosmetic products have emerged that provide instant results but ignore the health aspects of the user [2]. Natural ingredients for medical and cosmetic purposes are increasingly being used in developed and developing countries [3]. This is due to increasing public literacy regarding natural ingredients, where natural ingredients will minimize side effects. The natural ingredients in question can be obtained from microalgae [4]. Microalgae can produce primary compounds such as protein, carbohydrates, and fat. Microalgae also contain active components that can be used in industrial fields such as food, pharmaceuticals, nutraceuticals, and cosmetics [5]. Microalgae contain active components such as antimicrobial, antifungal, and antiviral activities, as well as antioxidants and antibacterial substances [6]. Chlorella and spirulina are natural antioxidants that have the potential to delay, slow down, and prevent the oxidation process [7].

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Section: Extraction Processcontrasting

confidence: 78%

Evaluation and Comparison Anti-aging Facial Serum from Algae Extract

Permadi,

Aziz,

Ramadani

et al. 2024

CHEMICA: Jurnal Teknik Kimia

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“…Pemutusan ikatan glikosida menjadi steroid dan glikon dapat dilakukan dengan hidrolisis menggunakan larutan HCl 2 N [19]. Partisi dilakukan untuk mengambil metabolit sekunder yang diinginkan yang mempunyai kesamaan kepolaran dengan pelarut [20]. Tonius dkk.…”

Section: Pendahuluanunclassified

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Fasya

Purwantoro

Ulya

et al. 2020

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Hydrilla verticillata is one of Allah SWT creations that potential as an antioxidant. This study’s objectives were to determine antioxidant activity and identify steroid isolates of n-hexane fraction of H. verticillata. The steroid compounds were extracted using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using n-hexane. The n-hexane fraction was tested phytochemically and separated using thin-layer chromatography (TLC). The steroids compounds were identified using UV-Vis spectrophotometer, FTIR, and LC-MS/MS. The result showed that maceration extraction produced 5.14% yield, whereas n-hexane fraction produce 47.95% yield. Steroid separation through analytical TLC revealed that n-hexane: ethyl acetate (4:1) as the best eluant with 12 spots, while separation using preparative TLC yielded 19 spots. Steroid isolate from TLC preparative has antioxidant activity with EC50 of 5.109 ppm. Identification of steroid compounds using UV-Vis produced maximum wavelengths at 203.9 and 276 nm, while using FTIR indicated the presence of O-H group, geminal dimethyl, C=O, C=C, secondary C-OH, and =C-H (alkene) which might contain steroid compounds. The result of LC-MS/MS showed the presence of β-sitosterol. Hydrilla verticillata merupakan salah satu ciptaan Allah SWT yang memiliki potensi sebagai antioksidan. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan mengidentifikasi senyawa steroid pada isolat hasil pemisahan fraksi n-heksana H. verticillata. Senyawa steroid diekstraksi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi dengan n-heksana. Fraksi n-heksana hasil partisi diuji fitokimia dan dipisahkan senyawanya menggunakan KLT (Kromatografi Lapis Tipis). Identifikasi dilakukan menggunakan UV-Vis, FTIR, dan LC-MS/MS. Hasil penelitian menunjukkan ekstraksi maserasi menghasilkan rendemen 5,14%, sedangkan rendemen partisi n-heksana sebesar 47,95%. Pemisahan steroid menggunakan KLT analitik menunjukkan bahwa variasi eluen terbaik adalah n-heksana: etil asetat (4:1) dengan 12 noda, sedangkan pemisahan KLT preparatif menghasilkan 19 noda. Isolat steroid hasil KLT preparatif memiliki aktivitas antioksidan dengan EC50 5,109 ppm. Identifikasi senyawa steroid menggunakan UV-Vis menunjukkan bahwa panjang gelombang maksimum sebesar 203,9 dan 276 nm, sedangkan untuk identifikasi isolat steroid menggunakan FTIR menunjukkan gugus fungsi O-H, geminal dimetil, C=O, C=C, C-OH sekunder dan =C-H (alkena) yang diduga merupakan senyawa steroid. Hasil LC-MS/MS menunjukkan adanya senyawa steroid β-sitosterol.

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“…Some secondary metabolites are bonded with sugar as glicosides, so can be extracted using polar solvent such as ethanol [18]. Separation can be done by hydrolysis using an acid solution to break glycosides bonds between glycon an aglycon [13], then partition using a semipolar or nonpolar solvent to separate the steroids [19]. More specific steroids separation can be done using column chromatography.…”

Section: Introductionmentioning

confidence: 99%

Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata

Fasya¹,

Amalia²,

Megawati³

et al. 2021

Advances in Social Science, Education and Humanities Research

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Hydrilla verticillata is one of the aquatic plants that contain some secondary metabolites, such as triterphenoids and steroids. The aim of this study was to determine the toxicity levels of steroids isolated from ethyl acetate fraction of Hydrilla verticillata separated by column chromatoghraphy. The biomass of Hydrilla verticillata was dried and powdered. Hydrilla verticillata powder is then extracted by maceration methods using ethanol solvent. The crude ethanol extracts were hydrolyzed using 2 N of HCl and partitioned using ethyl acetate solvent. The steroids compounds of ethyl acetate fraction were separated by Column Chromatography. The steroid isolates from Column Chromatography were identified by FTIR and UV-Vis spectrophotometer. The toxicity level of Column Chromatography steroid isolates was determined by BSLT method. The result of this research showed that extraction through maceration using ethanol produced 2.52 % yield, whereas the percent yield of the partition using ethyl acetate was 45.04 %. Separation by column chromatography resulted 3 steroids isolates B1, B2, and G1. The toxicity test showed that column chromatography steroids isolate from ethyl acetate fraction of Hydrilla verticillata has toxicity properties. The LC50 value of B1, B2 and G1 isolate was 14.58, 7.55 and 4.71 ppm, respectively.

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Ekstraksi, Hidrolisis dan Partisi Metabolit Sekunder dari Mikroalga Chlorella sp.

Cited by 5 publications

References 0 publications

Evaluation and Comparison Anti-aging Facial Serum from Algae Extract

Evaluation and Comparison Anti-aging Facial Serum from Algae Extract

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata

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