Eksplorasi Fusarium Nonpatogen untuk Pengendalian Penyakit Busuk Pangkal pada Bawang Merah

Isniah, Umi Sallamatul; Widodo, Widodo

doi:10.14692/jfi.11.1.14

Cited by 14 publications

(13 citation statements)

References 9 publications

(12 reference statements)

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“…Bulb/basal rot disease caused by Fusarium oxysporum (Isniah and Widodo 2015) is one of the most devastating diseases in shallot (Allium cepa var. aggregatum) causing severe yield losses of up to 100% (Dean et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots

Herlina¹,

Reflinur²,

Nugroho³

et al. 2018

J. AgroBiogen

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<p>Shallot (Allium cepa var. aggregatum) is one of the most important vegetable crops grown in Indonesia. The limited knowledge available on the genetic diversity and the threat of plant disease have been major problems to maintain high shallot production in Indonesia. Development of molecular markers linked to disease resistance is required for molecular breeding activity in this crop. This study aimed to assess the genetic diversity at conserved domain of resistance gene analog (RGA) in a set of 36 Indonesian shallot genotypes to complement morphological characterization. Twelve morphological and fifteen molecular markers traits were investigated in an attempt to characterize and to discriminate the Indonesian shallots genotypes. Characterization at orphological level indicated that phenotypic variance was highest for total bulb weight (TWB, cv = 99.39%) and the least for the plant height (PH, cv = 28.16%). The correlation analysis between traits showed that TWB and number of bulb (NB), TWB and bulb weight per plant (WB), NB and WB, and WB and PH were positively correlated. Molecular analysis revealed a total of 1,512 alleles with an average of 1.946 alleles per locus. The Polymorphism Information Content (PIC) values ranged from 0.253 to 0.676 and six out of 15 RGA markers were highly informative with PIC values ≥0.50. Based on cluster analysis, the 36 Indonesian shallot genotypes were clearly discriminated into six major groups. These results revealed that the RGA-based markers could support the morphological characterization in evaluating the genetic diversity of shallots. </p>

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots

Herlina¹,

Reflinur²,

Nugroho³

et al. 2018

J. AgroBiogen

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“…The disinfected sections were cut into 5 mm size, then incubated for 14 days at 25 o C in selective agar media. Emerging colonies were observed, and the hyphal tips of the colonies were transferred to potato dextrose agar (PDA) to obtain pure isolate culture [6]. Representative isolates were maintained on PDA plants.…”

Section: Identification Of the Causal Agent Of Fusarium Diseasementioning

confidence: 99%

“…Growth tests were carried out in a screen house, with pathogenic inoculation methods referring to [6]. Seedlings in the form of bulbs that were ready for planting (have stored through shelf life of 3 to4months) were sterilized from microbes by surface sterilization treatment and soaking treatmentin 55 o C sterile-water for 20 minutes.…”

Section: Growth Testmentioning

confidence: 99%

The Causal Agent of Fusarium Disease Infested Shallots in Java Islands of Indonesia

2021

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The success of disease control is largely determined by the validity of information about the type of causal-pathogen. Fusarium oxysporum f. sp. cepae (FOC) was reported to attack shallots and cause leaf twisting disease in Indonesia. We have conducted researches to investigate the causal agent of Fusarium disease and carried out growth tests on nine genotypes of shallots to determine the disease severity and disease indexes, and the correlation between diseases several agronomic traits. Thirty-five strains of Fusarium spp were isolated from wilted shallots tree and bulbs. Based on morphological characterization and rapid test to the isolates, they were identified as F oxysporum, F verticillioides, F solani and F proliferatum. The shallot genotypes that showed a tolerant response to FOC isolates tested were only Sumenep variety. Mentes, Tajuk, Batu Ijo, and Bima Brebes varieties show susceptible response.

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“…Adapun hama dan penyakit utama yang menyerang adalah Fusarium oxsporum (penyakit moler), dan serangan hama ulat perusak daun (Spodoptera exigua). Isniah dan Widodo (2015); Fadhilah et al (2014), melaporkan Fusarium oxysporum f sp. cepae merupakan patogen utama bawang merah, hasil eksplorasi didapatkan 18 isolat non patogen dan 3 isolat yang bersifat patogen terhadap bawang merah.…”

Section: Pendahuluanunclassified

Pengelolaan Tanaman Bawang Merah Ramah Lingkungan dengan Pemanfaatan Biopestisida Trichoderma

Manan¹,

Nurtiati²,

Mugiastuti³

2018

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Kelompok tani “Rukun Tani I” Desa Sumbang , Kecamatan Sumbang Kabupaten Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman hortikultur. Permasalahan utama yang dihadapi dalam peningkatan produksi bawang merah adalah tingginya serangan penyakit moler (Fusarium oxysporum) dan hama ulat bawang (Spodoptera exigua). Sosialisasi pengelolaan hama dan penyakit bawang merah dengan menggunakan biopestisida Trichoderma sp. perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang hama dan penyakit bawang merah serta tindakan pengelolaannya dengan pestisida hayati berbasis Trichoderma. Permasalahan mitra diatasi dengan beberapa pemecahan teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis hama dan penyakit bawang merah, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan aplikasi Trichoderma mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 % dan 34,09 %. Aplikasi pestisida hayati Trichoderma sp. pada petak demplot mampu meningkatkan pertumbuhan dan hasil bawang merah sebesar 7,09 % dan menurunkan intensitas hama dan penyakit sebesar 34,16 %.

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Eksplorasi Fusarium Nonpatogen untuk Pengendalian Penyakit Busuk Pangkal pada Bawang Merah

Cited by 14 publications

References 9 publications

Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots

Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots

The Causal Agent of Fusarium Disease Infested Shallots in Java Islands of Indonesia

Pengelolaan Tanaman Bawang Merah Ramah Lingkungan dengan Pemanfaatan Biopestisida Trichoderma

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