Eksplorasi Bakteri Actinomycetes Asli Papua Barat Sebagai Pewarna Makanan Alami dan Antimikroba

Abstract

Bakteri Actinomycetes merupakan kelompok bakteri yang memproduksi senyawa bahan alam (natural product) dan senyawa metabolit seperti pigmen dan antimikroba. Penelitian ini dilakukan untuk mengisolasi, mengidentifikasi produksi pigmen, serta aktivitas antimikroba Actinomycetes dari gundukan tanah dan sampah dari Kabupaten Fakfak, Papua Barat. Hasil yang diperoleh dari isolasi bakteri Actinomycetes adalah dua isolat, yaitu isolat pertama memiliki karakteristik morfologi yaitu hifa aerial berwarna putih, hifa substrat berwarna kuning, pigmen terlarut berwarna kekuningan dan spora yang berserbuk. Isolat kedua memiliki karakteristik morfologi yaitu hifa aerial berwarna putih kecoklatan, hifa substrat berwarna coklat, pigmen terlarut tidak ada dan spora yang berserbuk. Produksi pigmen yang berasal dari bakteri Actinomycetes asli Kab. Fakfak, Provinsi Papua Barat berpotensi menghasilkan pigmen karoten yang ditandai dengan hifa substrat yang berwarna kuning. Adapun zona hambat bakteri Staphylococcus aureus isolat ITP-1 sebesar 4,31 ± 0,17 mm dan isolat ITP-2 sebesar 2,51 ± 0,23. Zona hambat bakteri Escherichia coli isolat ITP-1 sebesar 3,23 ± 0,53 mm dan isolat ITP-2 sebesar 2,24 ± 0,26 mm.

Potential of Actinomycetes from Bora Hot Springs Central Sulawesi to Produce Antibacterial Compounds

“…This result in-line with previous research by Sulistyanto & Trimulyono [4]. The differences that occur in each character of Actinomycetes isolates are caused by the different biological role of each isolate [5]. Macroscopic observations data are presented in Fig.…”

“…Colony morphology observation through the observations according to the procedure that had been reported by Suloi et al, there were isolates that had white aerial mycelium, brownish white, and brown substrate mycelium. This is in-line with isolates AB-2, AB-3, AB-4 and AB-5 [5]. Another similarity with previous research are isolates AB-1 and AB-4 with convex elevation, isolates AB-2 and AB-5 with white colonies.…”

“…Based on the data above, it can be seen that the inhibition zone produced after being incubated for 24 h at 37 °C, there were only 2 isolates (AB-1 and AB-3) which showed antibacterial activity and were able to inhibit both pathogenic bacteria. The inhibition zone is formed due to the presence of antibacterial compounds resulting from secondary metabolites which are secreted into media that can inhibit growth and kill pathogenic bacteria [5].…”