Einige physikochemische Eigenschaften des Kartoffel-virus M

Proll, E.; Leiser, Robert-Matthias; Ostermann, Wolf-Dieter; Spaar, Dieter

doi:10.1007/bf02362012

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…The virus was purified from Nicotiana clevelandii plants 3 weeks after inoculation as described by Hollings & Stone (1972). Viral RNA was isolated from the purified virus preparation using the phenol-SDS method (Proll et al, 1981) with minor modifications.…”

Section: Introduction Methodsmentioning

confidence: 99%

Nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus B genomic RNA

Levay¹,

Завриев²

1991

Journal of General Virology

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Section: Introduction Methodsmentioning

confidence: 99%

Nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus B genomic RNA

Levay¹,

Завриев²

1991

Journal of General Virology

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“…PVM (Russian wild strain) was purified from infected tomato plants as described by ProU et al (1981). RNA was isolated from the purified virus preparation using the phenol-SDS extraction method (Proll et al, 1981) with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

“…Potato virus M (PVM), a member of the carlavirus group, has filamentous particles composed of multiple copies of the coat protein and a monopartite plus-sense ssRNA of approximately 8 kb in length (Proll et al, 1981;Wetter & Milne, 1981;Tavantzis, 1984). In our previous papers we published the nucleotide sequence of the 3'-proximal 2630 nucleotides of PVM genomic RNA and demonstrated a similarity between PVM and the potexviruses in the gene arrangement and encoded amino acid sequences (Rupasov et al, 1989(Rupasov et al, , 1990.…”

Section: Introductionmentioning

confidence: 99%

The Genome Organization of Potato Virus M RNA

Завриев

Kanyuka

Levay

1991

Journal of General Virology

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The 8534 nucleotide sequence of the genome of the carlavirus, potato virus M (PVM), has been determined. The sequence contains six large open reading frames (ORFs) and non-coding regions consisting of 75 nucleotides at the 5' end, 70 nucleotides followed by a poly(A) tail at the 3' end and 38 and 21 nucleotides between three large blocks of coding sequences. The ORF beginning at the first initiation codon at nucleotide 76 encodes a polypeptide of 223K which, according to its primary sequence analysis, seems to be a virus RNA replicase. The next coding block consists of three ORFs encoding polypeptides of 25K, 12K and 7K. The third block consists of two ORFs encoding polypeptides of 34K (PVM coat protein) and llK. The llK polypeptide contains a pattern resembling the consensus for a metal-binding nucleic acid-binding 'finger'. The nucleotide sequence EMBL accession number is X53062.

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