Addition of oxygen-containing C,-compounds to chemostat cultures of GB 25 increases both the yield of biomass and the specific growth rate. At optimum concentrations the catalytic activity of these compounds increases with increasing growth rates. Their influence on maintenance coefficients and maximum yield coefficients decreases in the order CH,OH > CO, > HCOOH > HCHO. This result together with spectrophotometric NADH determinations suggests that the NADH pool determines the balance between the assimilatory and oxidative utilization of formaldehyde.Methanotrophe Bakterien oxidieren Methan enzymatisch stufenweise uber C,-Intermediate zu Kohlendioxid (BROWN et u2. 1964, JOHNSON