Eine T�pfelreaktion zum Nachweis von nativem Eiwei�

Feigl, F.; Anger, V.

doi:10.1007/bf01471882

Cited by 18 publications

(10 citation statements)

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“…When the isoleucine fraction from tlc in butanol-acetic acid-water was developed twice in ethanolwater (90:10, v/v), the sample was resolved into two ninhydrin-positive components. The compound which had the smallest R f gave a positive test for N-alkyl amino acids with p-nitrobenzoyl chloride-pyridine (Plattner and Nager, 1948) and for a secondary amine with nitroprusside-acetaldehyde (Feigl and Anger, 1937). The infrared spectrum of this component was essentially identical with the one for standard N-methylvaline.…”

Section: Results a N D Discussionmentioning

confidence: 91%

“…The slowest moving ninhydrin-positive component in butanol-acetic acid-water (60:20:20, v/ v/v) had the same chromatographic mobility as an authentic standard sample of N-methyldanine in this and two other solvent systems. This purified sample gave a positive test for N-alkyl amino acids with p-nitrobenzoyl chloride-pyridine (Plattner and Nager, 1948) and for a secondary amine with nitroprusside-acetaldehyde (Feigl and Anger, 1937). The color produced by reaction with ninhydrin is the same rare reddish brown seen with standard N-methylalanine and N-methylglycine (sarcosine) (Eloff and Grobbelaar, 1967).…”

Section: Results a N D Discussionmentioning

confidence: 94%

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Purification and characterization of roseotoxin B, a toxic cyclodepsipeptide from Trichothecium roseum

Engstrom¹,

DeLance²,

Richard³

et al. 1975

J. Agric. Food Chem.

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Section: Results a N D Discussionmentioning

confidence: 91%

Section: Results a N D Discussionmentioning

confidence: 94%

Purification and characterization of roseotoxin B, a toxic cyclodepsipeptide from Trichothecium roseum

Engstrom¹,

DeLance²,

Richard³

et al. 1975

J. Agric. Food Chem.

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“…A strong absorption band near 45,000 cm-I is al 0 indicated. Two minima appear n ear 255 mj1 (39,200 cm -I ) and 325 mJ.l. (3 0,800 cm-I ) , with the respective indexes 9,000 and 6,400.…”

Section: Acid-base Reactions In Organic Solventsmentioning

confidence: 97%

“…In a similar connection, it has been the subject of spectrographic studies by Thiel and Diehl [36], and by Madame RamartLucas and her collaborators [37,38]. The dye and its potassium salt have been proposed for the detection of native protein [39].…”

Section: History Of Bromphthalein Magentamentioning

confidence: 99%

Acid-base reactions in benzene and other organic solvents: Behavior of bromphthalein magenta with different classes of organic bases

Davis¹,

Schuhmann²

1947

J. RES. NATL. BUR. STAN.

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Practical and theoretica l aspects of acid-base reactions in benzene and other organic solvents al'e discussed . The synthesis of the indicators bromphtha lein magenta E and B (the ethyl and n-butyl esters of t etrabromopheno lph thale in ) an d of their t etraalkylammonium salts is descri bed. Qualitative and spectrophotometric data concern in g the color reactions of the indicators with primary, seconda ry, and t ertia ry a li phatic amines , s ubstituted guan idines, a nd other n itrogen bases in benz ene and other types of organic solvents are presen ted. An expla nati on off ered for the color phenom ena is also appli cable to ulfon ephthalcins and other indicators.Examples are given of the qu antitative m eas urement of the relative reactivity of bases with bromphthal ein magenta in benzene. Illust rations a re furnishe d of the app li cation of the ind icato rs to the quantitative estimation of acids and bases, to measu rem ents of the relative s trengt,h s of orga ni c acids, and to the differen tiation o f primary, secondary, and tertiary a liphatic a mines.During t he sp ectrophotometric inves t igations, it was necessar y to des ign new types of a bsorption cells s uitable fo r vo latile solvents a nd for th in laye rs of solu t ion. A desc ription of the e cells is given.

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“…The copper complex of methylamphetamine gave a single spot with the R f value of 0.30, while the R f value of methylamphetamine was 0.18. Methylamphetamine (but not the copper complex) turned to violet when the Simon reagent 8,9 was spread over (Note that, unfortunately, the detection limit of methylamphetamine for this coloring test is at most 10 mg/ml or larger in solution 3 ). The spot of copper complex of methylamphetamine was extracted with acetone and evaporated to dryness.…”

Section: Identification Of the Copper Complex Of Methylamphetaminementioning

confidence: 99%

Determination of Methylamphetamine in Urine by Differential Pulse Polarography

Fushinuki

Taniguchi

1998

ANAL. SCI.

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A new electroanalytical method for detecting methylamphetamine as a stimulant drug in human urine has been proposed for forensic purposes. Although methylamphetamine itself has no remarkable voltammetric waves at any electrode in various electrolytes, we have found that the copper complex of methylamphetamine obtained by the method of Feigl showed a well-defined peak at -0.56 V (vs. SCE) at a mercury electrode by differential pulse polarography (DPP) in an aqueous ethanol solution containing 0.25 M ammonium acetate. The peak height was linear as a function of the methylamphetamine concentration in the original test solutions in the region of at least 0 -50 µg/ml. The concentrations of methylamphetamine determined by the present method for eight abusers' urine samples were in excellent agreement with those obtained by the conventional methods (GC-MS analysis) so far used. The proposed method is rather simple in procedure, rapid and selective with a satisfactory detection limit (<0.125 mg/ml) for analysis of methylamphetamine, and would also be applicable for field use.

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Eine T�pfelreaktion zum Nachweis von nativem Eiwei�

Cited by 18 publications

References 0 publications

Purification and characterization of roseotoxin B, a toxic cyclodepsipeptide from Trichothecium roseum

Purification and characterization of roseotoxin B, a toxic cyclodepsipeptide from Trichothecium roseum

Acid-base reactions in benzene and other organic solvents: Behavior of bromphthalein magenta with different classes of organic bases

Determination of Methylamphetamine in Urine by Differential Pulse Polarography

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