Eine neue Biosensor Plattform für den Einsatz in der Tierdiagnostik

Ewald, Melanie; Blanc, Alexander Le; Frimmer, V.; Gauglitz, G.

doi:10.5162/10dss2011/1.2

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2012

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“…Therefore, the antigen, a lipopolysaccharide, was immobilized onto the surface and the antigen-antibody interaction was monitored in a time-resolved manner by Reflectometric Interference Spectroscopy (RIfS). The new assay including the surface chemistry and assay procedure was set up and fully characterized 29 . The calibration curve is presented in Figure 12.…”

Section: Healthcarementioning

confidence: 99%

Optical sensing: recognition elements and devices

Gauglitz

2012

SPIE Proceedings

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The requirements in chemical and biochemical sensing with respect to recognition elements, avoiding non-specific interactions, and high loading of the surface for detection of low concentrations as well as optimized detection systems are discussed. Among the many detection principles the optical techniques are classified. Methods using labeled compounds like Total Internal Reflection Fluorescence (TIRF) and direct optical methods like micro reflectometry or refractometry are discussed in comparison. Reflectometric Interference Spectroscopy (RIfS) is presented as a robust simple method for biosensing. As applications, trace analysis of endocrine disruptors in water, hormones in food, detection of viruses and bacteria in food and clinical diagnostics are discussed.

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Section: Healthcarementioning

confidence: 99%

Optical sensing: recognition elements and devices

Gauglitz

2012

SPIE Proceedings

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Eine neue Biosensor Plattform für den Einsatz in der Tierdiagnostik

Cited by 1 publication

References 2 publications

Optical sensing: recognition elements and devices

Optical sensing: recognition elements and devices

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