Optical sensing: recognition elements and devices

Gauglitz, Güenter

doi:10.1117/12.970550

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…In recent years, many research groups have used laser-induced fluorescence in microfluidic chips, such as lab-on-chip microsystems [ 8 ], capillary electrophoresis [ 9 , 10 , 11 ] or miniature biosensor devices [ 12 , 13 , 14 ]. Depending on the purpose and implementation of the tests, the determination is carried out in the volume of liquid (e.g., capillary electrophoresis) or on a functionalized surface [ 15 , 16 ]. One of the most frequently used mechanisms is Total Internal Reflection Fluorescence (TIRF), which stimulates fluorescently determined analytes on the surface of the flow channel using a evanescent waveguide [ 17 , 18 , 19 , 20 ].…”

Section: Point-of-care—vital Body Fluids’ Agentsmentioning

confidence: 99%

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

Nawrot

Drzozga

Baluta

et al. 2018

Sensors

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The clinical applications of sensing tools (i.e., biosensors) for the monitoring of physiologically important analytes are very common. Nowadays, the biosensors are being increasingly used to detect physiologically important analytes in real biological samples (i.e., blood, plasma, urine, and saliva). This review focuses on biosensors that can be applied to continuous, time-resolved measurements with fluorescence. The material presents the fluorescent biosensors for the detection of neurotransmitters, hormones, and other human metabolites as glucose, lactate or uric acid. The construction of microfluidic devices based on fluorescence uses a variety of materials, fluorescent dyes, types of detectors, excitation sources, optical filters, and geometrical systems. Due to their small size, these devices can perform a full analysis. Microfluidics-based technologies have shown promising applications in several of the main laboratory techniques, including blood chemistries, immunoassays, nucleic-acid amplification tests. Of the all technologies that are used to manufacture microfluidic systems, the LTCC technique seems to be an interesting alternative. It allows easy integration of electronic and microfluidic components on a single ceramic substrate. Moreover, the LTCC material is biologically and chemically inert, and is resistant to high temperature and pressure. The combination of all these features makes the LTCC technology particularly useful for implementation of fluorescence-based detection in the ceramic microfluidic systems.

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Section: Point-of-care—vital Body Fluids’ Agentsmentioning

confidence: 99%

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

Nawrot

Drzozga

Baluta

et al. 2018

Sensors

View full text Add to dashboard Cite

show abstract

“…Depending on the purpose and the implementation, the detection is either performed in the fluid volume (e.g. capillary electrophoresis) or on a functionalized surface [11][12][13]. As an example, total internal reflection fluorescence (TIRF) [14][15][16][17] is a powerful method that excites the fluorescent labeled analytes on a flow channel surface using evanescent waveguiding.…”

Section: Introductionmentioning

confidence: 99%

Laser-induced fluorescence detection platform for point-of-care testing

Berner

Hilbig

Schubert

et al. 2017

Meas. Sci. Technol.

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Point-of-care testing (POCT) devices for continuous low-cost monitoring of critical patient parameters require miniaturized and integrated setups for performing quick high-sensitivity analyses, away from central clinical laboratories. This work presents a novel and promising laser-induced fluorescence platform for measurements in direct optical test formats that leads towards such powerful POCT devices based on fluorescence-labeled immunoassays. Ultimate sensitivity of thin film photodetectors, integrated with microfluidics, and a comprehensive optimization of all system components aim at low-level signal detection in the targeted biosensor application. The setup acquires fluorescence signals from the volume of a microfluidic channel. An innovative sandwiching process forms a flow channel in the microfluidic chips by embedding laser-cut double-sided adhesive tapes. The custom fit of amorphous silicon based photodiode arrays to the geometry of the flow channel enables miniaturization, fully adequate for POCT devices. A free-beam laser excitation with line focus provides excellent alignment stability, allows for easy and reliable swapping of the disposable microfluidic chips, and therewith greatly improves the ease of use of the resulting integrated device. As a proof-of-concept of this novel in-volume measurement approach, the limit of detection for the dye DY636-COOH in pure water as a model fluorophore is examined and found to be 26 nmol .

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Optical sensing: recognition elements and devices

Cited by 2 publications

References 21 publications

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

Laser-induced fluorescence detection platform for point-of-care testing

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