Ein neues Reagenz zum Nachweis und zur Unterscheidung von Flavonen im Papierchromatogramm

Neu, Richard

doi:10.1007/bf00631858

Cited by 57 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, phenolic compound histolocalization methods mainly uses the standard reagent for phenols and flavonoids, the Neu’s reagent, and then examination by epifluorescence microscopy [36,37]. Excitation with UV laser beam at 364 nm induced strong green fluorescence in the epidermal cells of coffee leaves, which was attributed to the presence of phenolic compounds [24].…”

Section: Resultsmentioning

confidence: 99%

Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

2015

View full text Add to dashboard Cite

Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment.

show abstract

Section: Resultsmentioning

confidence: 99%

Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

2015

View full text Add to dashboard Cite

show abstract

“…The presence of alkaloids, terpenoids, steroids, saponins, iridoids, coumarins, cinnamic derivatives, phenylpropanoglucosides, flavonoids, phenolic acids, condensed proanthocyanidins, leucoanthocyanidins, and hydrolysable tannins was investigated using adequate development systems and revealers as listed in Table 1 (Harborne, 1998;Markhan, 1982;Neu, 1956;Roberts et al, 1957;Wagner & Bladt, 1996;Wallenfels, 1950). After development, the plates were air dried and sprayed with the indicators in a fume hood.…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Nova

Almeida

Procópio

et al. 2022

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

This study evaluated the effects of acute exposure of Aedes aegypti third instar (L3) larvae to the saline extract of Opuntia ficus‐indica cladodes on the biological cycle and fertility of the emerging adults. For this, larvae were treated for 24 h with the extract at ¼ LC50 (lethal concentration to kill 50% of larvae), ½ LC50 or LC50; the development and reproduction of the emerged adults were evaluated after a recovery period of 9 days. The resistance of proteins in the extract to hydrolysis by L3 digestive enzymes and histomorphological alterations in the larval midgut were also investigated. The extract contained lectin, flavonoids, cinnamic derivatives, terpenes, steroids, and reducing sugars. It showed a LC50 of 3.71% for 48 h. The data indicated mean survival times similar in control and extract treatments. It was observed development delay in extract‐treated groups, with a lower number of adults than in control. However, the females that emerged laid similar number of eggs in control and treatments. Histological evaluation revealed absence of bacterial and fungal microorganisms in the food content in midguts from larvae treated with cladode extract. Electrophoresis revealed that three polypeptides in the extract resisted to hydrolysis by L3 digestive proteases for 90 min. The lectin activity was not altered even after 24‐h incubation with the enzymes. In conclusion, the extract from O. ficus‐indica can delay the development of Ae. aegypti larvae, which may be linked to induction of an axenic environment at larval midgut and permanence of lectin activity even after proteolysis.

show abstract

“…To date, phenol histolocalization is generally done using Neu's reagent, which binds with phenolics, emitting a specific greenish-white epifluorescence under UV light (Neu, 1956). As expected, the most intense greenish-white fluorescence is observed in juvenile coffee leaf blades (Mondolot et al, 2006; Campa et al, 2012), but its specificity toward HQA is low.…”

Section: Introductionmentioning

confidence: 99%

Spectral analysis combined with advanced linear unmixing allows for histolocalization of phenolics in leaves of coffee trees

et al. 2014

View full text Add to dashboard Cite

An imaging method using spectral analysis combined with advanced linear unmixing was used to allow histolocalization of natural autofluorescent compounds such as hydroxycinnamic acid (chlorogenic acid) and xanthone (mangiferin) in living cells and tissues (mature coffee leaves). The tested method included three complementary steps: 1/ visualization of natural autofluorescence and spectrum acquisition with a multiphoton microscope; 2/ identification of some compounds using previous information on the chemical composition of the tissue, obtained from litterature; and 3/ localization of candidate compounds by spectral imaging. The second part of the study consisted of describing the histochemical structure of leaves during their development. This revealed very fast histochemical differentiation of leaves during the first week after their emergence. Lastly, young leaves of Coffea pseudozanguebariae (PSE), C. eugenioides (EUG), C. arabica (ARA) and C. canephora (CAN) were compared. This confirmed the presence of xanthone in PSE and EUG, but especially its precise tissue localization. This also highlighted the paternal CAN origin of the leaf structure in the allotetraploid species ARA. The limits and advantages of the method without staining are discussed relative to classical epifluorescence microscopy under UV light. This non-invasive optical technique does not require pretreatment and is an effective experimental tool to differentiate multiple naturally-occuring fluorochores in living tissues.

show abstract

Ein neues Reagenz zum Nachweis und zur Unterscheidung von Flavonen im Papierchromatogramm

Cited by 57 publications

References 6 publications

Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Spectral analysis combined with advanced linear unmixing allows for histolocalization of phenolics in leaves of coffee trees

Contact Info

Product

Resources

About