Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

Talamond, Pascale; Verdeil, Jean‐Luc; Conéjéro, Geneviève

doi:10.3390/molecules20035024

Cited by 141 publications

(100 citation statements)

References 38 publications

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“…Fortunately, a wide range of plant metabolites (e.g. most phenolics, many alkaloids, many terpenoids, several co-factors and flavin coenzymes) exhibit autofluorescence with unique excitation/emission properties (Talamond et al, 2015). These autofluorescence properties provide an opportunity to investigate the dynamics of metabolites inside cells without the need to perturb the system with exogenous markers or fluorescent dyes.…”

Section: Introductionmentioning

confidence: 99%

Using fluorescence lifetime microscopy to study the subcellular localization of anthocyanins

et al. 2016

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Anthocyanins are flavonoid pigments that accumulate in most seed plants. They are synthesized in the cytoplasm but accumulate inside the vacuoles. Anthocyanins are pigmented at the lower vacuolar pH, but in the cytoplasm they can be visualized based on their fluorescence properties. Thus, anthocyanins provide an ideal system for the development of new methods to investigate cytoplasmic pools and association with other molecular components. We have analyzed the fluorescence decay of anthocyanins by fluorescence lifetime imaging microscopy (FLIM), in both in vitro and in vivo conditions, using wild-type and mutant Arabidopsis thaliana seedlings. Within plant cells, the amplitude-weighted mean fluorescence lifetime (τ ) correlated with distinct subcellular localizations of anthocyanins. The vacuolar pool of anthocyanins exhibited shorter τ than the cytoplasmic pool. Consistently, lowering the pH of anthocyanins in solution shortened their fluorescence decay. We propose that FLIM is a useful tool for understanding the trafficking of anthocyanins and, potentially, for estimating vacuolar pH inside intact plant cells.

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Section: Introductionmentioning

confidence: 99%

Using fluorescence lifetime microscopy to study the subcellular localization of anthocyanins

et al. 2016

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“…Figure 9 shows that the Mah ı samples are darker under UV. The observed fluorescence of Mah ı can be attributed to the presence of natural multicomponent fluorescent systems such as phenolic compounds and chlorophylls [70,71]. The observed fluorescence of Mah ı can be attributed to the presence of natural multicomponent fluorescent systems such as phenolic compounds and chlorophylls [70,71].…”

Section: M1amentioning

confidence: 91%

“…M1B M1C M8A M8B M8C Application of UV fluorescence imaging and fluorescence microscopy UV light induces a fluorescent nature in many natural products [70]. Fluorescence analysis of very dilute aqueous solutions of powdered Mah ı samples and stains of Mah ı on bond paper showed different coloration in the presence and absence of UV ranging from blue to green fluorescence (Figures 8 and 9; Figures S13 and S14).…”

Section: M1amentioning

confidence: 99%

“…But the samples did not show any kind of phosphorescence when the exposure to UV source was stopped. The blue fluorescence of plants is associated with the emissions of several universal cellular fluorophores including nicotinamide, flavin coenzymes, pyridoxal phosphate, folic acid and secondary metabolites including the three main groups, namely, phenolics, alkaloids and terpenoids [70]. The blue fluorescence of plants is associated with the emissions of several universal cellular fluorophores including nicotinamide, flavin coenzymes, pyridoxal phosphate, folic acid and secondary metabolites including the three main groups, namely, phenolics, alkaloids and terpenoids [70].…”

Section: M1amentioning

confidence: 99%

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A physicochemical characterisation of a medieval herbal ink, Mahī, of Assam, India

Goswami

Chamuah

Dutta

2018

Coloration Technology

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Mah ı, a unique herbal ink used in medieval Assam in India for writing and illustrating manuscripts, is known for its intense colour and durability for centuries, and its non-corrosive nature. Mah ı is distinctly different from the other contemporary inks used elsewhere in the world, including other parts of India. The interesting features of Mah ı are due to its special recipe involving several herbal ingredients in addition to iron sourced from fish blood or the rust of iron tools, with cow urine as an extractant and solvent. The objective of the current study is an in-depth understanding of the traditional method of the preparation of Mah ı, and its characterisation through analysis of the physicochemical properties using spectroscopic and imaging techniques as well as biochemical analysis. A series of model Mah ı samples are prepared following the traditional method with varying ingredients, and their compositions and physicochemical properties are evaluated with respect to the ingredients using standard methods and analytical tools including atomic absorption spectroscopy, tensiometry, ultraviolet ( UV )-visible, Fourier Transform-infrared ( FT-IR), Raman, steady-state fluorescence, UV fluorescence and emission microscopy. The colour of the ink has been attributed to various polyphenols, gallic acid, epigallocatechin gallate, epicatechin, quercetin, kaempferol and tannic acid, and their iron complexes. UV fluorescence and emission microscopy confirmed an autofluorescence indicative of the presence of phenolic and chlorophyll pigments. Solubilisation by glycosidic biosurfactant contributes to stabilisation of Mah ı. The non-corrosive nature of Mah ı has been attributed to its neutral pH and absence of free iron and copper ions.

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“…Such was the case for ferulic acid [64,65] or chlorogenic acid [66]. More recently, Talamond et al [67] addressed the location of many phenolics using multiphoton microscopy. In leaves of healthy plants, the strongest BGF corresponds to the epidermis, vascular tissue and also mesophyll cell walls.…”

Section: Multicolor Fluorescence Imagingmentioning

confidence: 99%

Picturing pathogen infection in plants

Barón

Pineda

Pérez-Bueno

2016

Zeitschrift Für Naturforschung C

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Several imaging techniques have provided valuable tools to evaluate the impact of biotic stress on host plants. The use of these techniques enables the study of plant-pathogen interactions by analysing the spatial and temporal heterogeneity of foliar metabolism during pathogenesis. In this work we review the use of imaging techniques based on chlorophyll fluorescence, multicolour fluorescence and thermography for the study of virus, bacteria and fungi-infected plants. These studies have revealed the impact of pathogen challenge on photosynthetic performance, secondary metabolism, as well as leaf transpiration as a promising tool for field and greenhouse management of diseases. Images of standard chlorophyll fluorescence (Chl-F) parameters obtained during Chl-F induction kinetics related to photochemical processes and those involved in energy dissipation, could be good stress indicators to monitor pathogenesis. Changes on UV-induced blue (F440) and green fluorescence (F520) measured by multicolour fluorescence imaging in pathogen-challenged plants seem to be related with the up-regulation of the plant secondary metabolism and with an increase in phenolic compounds involved in plant defence, such as scopoletin, chlorogenic or ferulic acids. Thermal imaging visualizes the leaf transpiration map during pathogenesis and emphasizes the key role of stomata on innate plant immunity. Using several imaging techniques in parallel could allow obtaining disease signatures for a specific pathogen. These techniques have also turned out to be very useful for presymptomatic pathogen detection, and powerful non-destructive tools for precision agriculture. Their applicability at lab-scale, in the field by remote sensing, and in high-throughput plant phenotyping, makes them particularly useful. Thermal sensors are widely used in crop fields to detect early changes in leaf transpiration induced by both air-borne and soil-borne pathogens. The limitations of measuring photosynthesis by Chl-F at the canopy level are being solved, while the use of multispectral fluorescence imaging is very challenging due to the type of light excitation that is used.Keywords: biotic stress; chlorophyll fluorescence imaging; multicolor fluorescence imaging; plant pathogens; thermography. Dedication: This work is dedicated to the memory of Professor Peter Böger. He will always bring to my heart (M. B.) the best memories of my postdoc at Lake Constance.

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Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

Cited by 141 publications

References 38 publications

Using fluorescence lifetime microscopy to study the subcellular localization of anthocyanins

Using fluorescence lifetime microscopy to study the subcellular localization of anthocyanins

A physicochemical characterisation of a medieval herbal ink, Mahī, of Assam, India

Picturing pathogen infection in plants

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