Eight flavonoids and their potential as inhibitors of human cytomegalovirus replication

Cotin, Sébastien; Calliste, Claude-Alain; Mazeron, Marie‐Christine; Hantz, Sébastien; Duroux, Jean‐Luc; Rawlinson, William D.; Ploy, Marie-Cécile; Alain, Sophie

doi:10.1016/j.antiviral.2012.09.010

Cited by 58 publications

(37 citation statements)

References 18 publications

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“…1, which endows it with stronger affinity to proteins (Cotin et al, 2012). Limited studies revealed that quercetagetin exhibited a range of pharmacological activities.…”

Section: Introductionmentioning

confidence: 99%

Physical, structural, thermal and morphological characteristics of zeinquercetagetin composite colloidal nanoparticles

Sun

Liu

Yang

et al. 2015

Industrial Crops and Products

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“…1, which endows it with stronger affinity to proteins (Cotin et al, 2012). Limited studies revealed that quercetagetin exhibited a range of pharmacological activities.…”

Section: Introductionmentioning

confidence: 99%

Physical, structural, thermal and morphological characteristics of zeinquercetagetin composite colloidal nanoparticles

Sun

Liu

Yang

et al. 2015

Industrial Crops and Products

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“…The extract did not inhibit the development of the HSV-1 induced cytopathic effect (rounded cells and production of syncytium) at this concentration, as observed in figure 2. Regarding the compounds identified in the phytochemical characterization, there are reports of antiviral activity against HSV-1 for purified flavonoids quercetin and naningerin (Cotin et al, 2012) evaluated by the ortho-nitrophenyl-β-Dgalactopyranoside (ONPG) method, which measures the percentage of surviving cells in relation to an infected control. Differences in the methods employed to evaluate the antiviral activity as well as possible lower concentrations of these compounds in the methanol extract of F. vasta or even interactions of the different molecules present in the extract with them could perhaps explain the absence of anti-HSV-1 activity observed in this study.…”

Section: Antibacterial Assaymentioning

confidence: 99%

Evaluation of cytotoxicity, anti-herpes simplex virus type 1 (HSV-1) and antibacterial activities of Ficus vasta and phytoconstituents

Rashed

Ono²

2013

Int Curr Pharm J

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This study deals with the evaluation of in vitro cytotoxicity of the methanol 80% extract of Ficus vasta aerial parts in Vero cells, anti-HSV-1 and antibacterial activities and the determination of the phytochemical content of the F. vasta methanol extract. The extract was tested for its cytotoxicity in Vero cells and for anti-HSV-1 and against bacterial strains as S. aureus, S. epidermidis, E. coli and P. aeruginosa and also phytochemical constituents were detected. The results showed that this extract presented a CC50 value of 389 µg/mL, an intermediate value taking into account other researchers reported for some Moraceae species. Regarding the other biological activities, antiviral effect was not demonstrated by a HSV-1 challenge dilution of 100x tissue culture infective dose 50% (TCID50) and also by the disk-diffusion method at the higher non-toxic concentration of the extract (119 µg/mL). Phytochemical analysis of the methanol extract proved the presence of carbohydrates, tannins, flavonoids, coumarins, alkaloids and triterpenes. Chromatographic separation of the methanol extract resulted in the isolation and identification of ?-sitosterol, stigmasterol, lupeol, ursolic acid, naringenin, luteolin, quercetin, vitexin, quercetin 3-O-?-glucoside and rutin. The results indicated that F. vasta aerial parts methanol extract is potential candidate for experiments of biological activity screening that are not anti-HSV-1 or antibacterial against the strains evaluated in this study, to be carried on in the future.DOI: http://dx.doi.org/10.3329/icpj.v3i1.17295 International Current Pharmaceutical Journal, December 2013, 3(1): 211-218

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“…In fact, virucidal activity might eclipse antiviral activity if tests of direct inactivation of viral particles are not performed. The antiviral activity of the flavonoids as quercetin, caffeic acid and kaempferol has been reported for others viruses, including porcine epidemic diarrhea virus, herpesvirus, human cytomegalovirus, and influenza virus (WANG et al, 1994;COTIN et al, 2012;AMOROS et al, 1992;CHOI et al, 2009;KUMAR et al, 2005;GRAVINA et al, 2011). The results show that the virucidal activity of the EEO is related to several chemical compounds.…”

Section: Resultsmentioning

confidence: 53%

“…Perhaps the reduction of virus yield using the cytopathic effect inhibition assay in RK13 also is due to inactivation of viral particles during morphogenesis or budding steps of the viral cycle. However, another mechanism of action may be existing since the quercetin, for example, known to be inhibitors of HIV-reverse transcriptase (WANG et al, 1994) and have been shown to be inhibitory to the activities of various cellular DNA and RNA polymerases (ONO; NAKANE, 1990;COTIN et al, 2012). Also it can be assumed that part of the antiviral activity of EEO relies The compounds flavonoids are promising candidates as they have low toxicity and probably inhibit different targets to currently available antivirals.…”

Section: Resultsmentioning

confidence: 99%

Anti-equine arteritis virus activity of ethanolic extract and compounds from Origanum vulgare

Blank

Corrêa

Freitag

et al. 2017

SCA

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The equine arteritis virus (EAV) is responsible by an important respiratory and reproductive disease in equine populations and there is no specific antiviral treatment available. The objective of this study was to investigate the activity of an ethanolic crude extract of Origanum vulgare (EEO) and of isolated compound caffeic acid, p-coumaric acid, rosmarinic acid, quercetin, luteolin, carnosol, carnosic acid, kaempferol and apigenin against EAV. The assays were performed using non-cytotoxic concentrations. The antiviral activity was monitored initially by cytopathic effect inhibition (CPE) assay in RK13 cells in the presence or absence of EEO. Pre-incubated cells with EEO were also examined to show prophylactic effect. Direct viral inactivation by EEO and isolated compounds was evaluated by incubation at 37°C or 20°C. After the incubation period, the infectivity was immediately determined by virus titrations on cell cultures and expressed as 50% tissue culture infective dose (TCID 50 )/100 µL. There was significant virucidal activity of EEO and of the compounds caffeic acid, p-coumaric acid, quercetin, carnosic acid and kaempferol. When EEO was added after infection, EEO inhibited the virus growth in infected cells, as evidenced by significant reduction of the viral titre. The results provide evidence that the EEO exhibit an inhibitory effect anti-EAV. Among the main compounds evaluated, caffeic acid, p-coumaric acid, carnosic acid, kaempferol and mainly quercetin, contributed to the activity of EEO. EEO may represent a good prototype for the development of a new antiviral agent, presenting promising for combating arteriviruses infections. Key words: Antimicrobial. Antiviral. Cytotoxicity. Origanum sp. Virucidal. ResumoO virus da arterite equine (EAV) é responsável por uma importante doença respiratória e reprodutiva na população de equinos e não há tratamento antiviral específico disponível. O objetivo desse estudo foi investigar a atividade de um extrato etanólico de Origanum vulgare (EEO) e dos compostos isolados ácido caféico, ácido p-cumarico, ácido rosmarínico, quercetina, luteolina, carnosol, ácido carnósico, campferol e apigenina contra o EAV. Os ensaios foram realizados com concentrações não citotóxicas. A atividade antiviral foi monitorada inicialmente por um ensaio de inibição de efeito citopático (CPE) em células RK13 na presença ou ausência do EEO. Células pré-incubadas com o EEO foram também examinadas para a presença de efeito profilático. Inativação direta do EAV pelo EEO e pelos compostos isolados foi avaliada mediante incubação a 37°C ou 20°C. Após o período de incubação, a infectividade foi imediatamente determinada por titulação viral em cultivo celular sendo expresso como dose infectiva a 50% (TCID 50 )/100 µL. Houve significativa atividade virucida do EEO e dos compostos ácido caféico, ácido p-cumarico, quercetina, ácido carnósico e campferol. Quando o EEO foi adicionado após a infecção, o EEO inibiu a replicação do vírus nas células infectadas, o que foi evidenciado pela redução s...

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Eight flavonoids and their potential as inhibitors of human cytomegalovirus replication

Cited by 58 publications

References 18 publications

Physical, structural, thermal and morphological characteristics of zeinquercetagetin composite colloidal nanoparticles

Physical, structural, thermal and morphological characteristics of zeinquercetagetin composite colloidal nanoparticles

Evaluation of cytotoxicity, anti-herpes simplex virus type 1 (HSV-1) and antibacterial activities of Ficus vasta and phytoconstituents

Anti-equine arteritis virus activity of ethanolic extract and compounds from Origanum vulgare

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