Eidetic Analysis of the Premature Chromosome Condensation Process

Rybaczek, Dorota

doi:10.5772/20811

Cited by 3 publications

(5 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite this, the induction of apoptosis occurs most frequently during drug-induced PCC (comp. [ 16 ]), which suggests the co-existence of a similar signal transduction pathway toward apoptosis and PCC [ 11 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Caffeine-Induced Premature Chromosome Condensation Results in the Apoptosis-Like Programmed Cell Death in Root Meristems of Vicia faba

2015

Self Cite

View full text Add to dashboard Cite

We have demonstrated that the activation of apoptosis-like programmed cell death (AL-PCD) was a secondary result of caffeine (CF) induced premature chromosome condensation (PCC) in hydroxyurea-synchronized Vicia faba root meristem cells. Initiation of the apoptotic-like cell degradation pathway seemed to be the result of DNA damage generated by treatment with hydroxyurea (HU) [double-stranded breaks (DSBs) mostly] and co-treatment with HU/CF [single-stranded breaks (SSBs) mainly]. A single chromosome comet assay was successfully used to study different types of DNA damage (neutral variant–DSBs versus alkaline–DSBs or SSBs). The immunocytochemical detection of H2AXS139Ph and PARP-2 were used as markers for DSBs and SSBs, respectively. Acridine orange and ethidium bromide (AO/EB) were applied for quantitative immunofluorescence measurements of dead, dying and living cells. Apoptotic-type DNA fragmentation and positive TUNEL reaction finally proved that CF triggers AL-PCD in stressed V. faba root meristem cells. In addition, the results obtained under transmission electron microscopy (TEM) further revealed apoptotic-like features at the ultrastructural level of PCC-type cells: (i) extensive vacuolization; (ii) abnormal chromatin condensation, its marginalization and concomitant degradation; (iii) formation of autophagy-like vesicles (iv) protoplast shrinkage (v) fragmentation of cell nuclei and (vi) extensive degeneration of the cells. The results obtained have been discussed with respect to the vacuolar/autolytic type of plant-specific AL-PCD.

show abstract

Section: Discussionmentioning

confidence: 99%

“…However, spindle formation, p34 cdc2 activation and phosphorylation of histones H1 and H3 occurred only in PCC, which indicates molecular differences between these processes (i.e. PCC versus apoptosis [ 11 – 12 , 16 ]).…”

Section: Introductionmentioning

confidence: 99%

Caffeine-Induced Premature Chromosome Condensation Results in the Apoptosis-Like Programmed Cell Death in Root Meristems of Vicia faba

2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…8 , 12 ) clearly demonstrating that general rules of normal cell cycle progression were well preserved here, indicating that CF, 2-AP, and Van induce PCC and all its attendant H3S10Ph (comp. Rybaczek 2011 ). The posttranslation modification of H3 histone consisting of phosphorylation at Ser10 (H3S10Ph) constitutes a preliminary signal of mitotic-type chromatin condensation and is tightly correlated with prophase initiation [in plant cells, the phosphorylation of Ser10 histone H3 takes place in prophase chromosomes which are already condensed (Kaszás and Cande 2000 ) and does not extend beyond the pericentromeric region (Houben et al 1999 ; Manzanero et al 2000 )].…”

Section: Discussionmentioning

confidence: 99%

“…However, the mechanisms inducing premature chromosome condensation (PCC) through overriding of the S-M checkpoint are relatively poorly recognized (Nghiem et al 2001; Rybaczek 2011 and ref. therein; Rybaczek and Kowalewicz-Kulbat 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Studies carried out on the organization of S-phase checkpoints elucidated the function of the main elements of the S-M checkpoint, whereby the capability of cells to divide mitotically in response to DNA replication arrest is blocked (Bartek et al 2004 ). However, the mechanisms inducing premature chromosome condensation (PCC) through overriding of the S-M checkpoint are relatively poorly recognized (Nghiem et al 2001 ; Rybaczek 2011 and ref. therein; Rybaczek and Kowalewicz-Kulbat 2011 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells

Rybaczek

2014

Plant Cell Rep

Self Cite

View full text Add to dashboard Cite

Key messagePCC induction is regulated by several signaling pathways, and all observed effects associated with PCC induction are strongly dependent on the mechanism of action of each PCC inducer used.AbstractElectron microscopic observations of cells with symptoms of premature chromosome condensation (PCC) showed that the interphase chromatin and mitotic chromosomes differed with respect to a chemical compound inducing PCC. Induction of this process under the influence of hydroxyurea and caffeine as well as hydroxyurea and sodium metavanadate led to a slight decrease in interphase chromatin condensation and the formation of chromosomes with a considerably loosened structure in comparison with the control. Incubation in the mixture of hydroxyurea and 2-aminopurine brought about clear chromatin dispersion in interphase and very strong mitotic chromosome condensation. Electron microscopic examinations also revealed the characteristic features of the structural organization of cytoplasm of Vicia faba root meristems, which seemed to be dependent on the type of the PCC inducer used. The presence of the following was observed: (i) large plastids filled with starch grains (caffeine), (ii) mitochondria and plastids of electron dense matrix with dilated invaginations of their internal membranes (2-aminopurine), and (iii) large mitochondria of electron clear matrix and plastids containing protein crystals in their interior (sodium metavanadate). Moreover, since caffeine causes either the most effective loosening of chromatin fibrils (within the prematurely condensed chromosomes) or induction of starch formation (in the plastids surrounding the nuclei), this may be a proof that demonstrates the existence of a link between physical accessibility to chromatin and the effectiveness of cellular signaling (e.g., phosphothreonine-connected).

show abstract

Relation of the Types of DNA Damage to Replication Stress and the Induction of Premature Chromosome Condensation

Rybaczek¹,

Kowalewicz-Kulbat²

2013

New Research Directions in DNA Repair

Self Cite

View full text Add to dashboard Cite

Eidetic Analysis of the Premature Chromosome Condensation Process

Cited by 3 publications

References 82 publications

Caffeine-Induced Premature Chromosome Condensation Results in the Apoptosis-Like Programmed Cell Death in Root Meristems of Vicia faba

Caffeine-Induced Premature Chromosome Condensation Results in the Apoptosis-Like Programmed Cell Death in Root Meristems of Vicia faba

Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells

Relation of the Types of DNA Damage to Replication Stress and the Induction of Premature Chromosome Condensation

Contact Info

Product

Resources

About