Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

Büyükgüzel, Ender; Hyršl, Pavel; Büyükgüzel, Kemal

doi:10.1016/j.cbpa.2010.01.020

Cited by 44 publications

(31 citation statements)

References 60 publications

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“…Fifteen newly emerged adult females were placed in jars and provided a piece of old honeycomb on the diet for egg deposition and feeding of newly hatched larvae. The methods used to prepare and dispense diets into container, and the methods used to obtain eggs and larvae and their placement onto diets were described in previous studies (Hyršl et al 2008; Büyükgüzel et al 2010). …”

Section: Methodsmentioning

confidence: 99%

Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Büyükgüzel

Snela

et al. 2013

Cell Biol Toxicol

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Boric acid is widely used as an insecticide, acaricide, herbicide, and fungicide and also during various industrial processings. Hence, numerous populations are subjects to this toxic compound. Its action on animals is still not fully known and understood. We examined the effect of boric acid on larvae of greater wax moth (Galleria mellonella). The chemical appeared to be toxic for larvae, usually in a concentration-dependent manner. Exposed groups revealed increased lipid peroxidation and altered activity of catalase, superoxide dismutase, glutathione S-transferase, and glutathione peroxidase. We also observed changes of ultrastructure, which were in tune with biochemical assays. We suggest that boric acid has a broad mode of action, which may affect exposed larvae, and even if sublethal, they may lead to disturbances within exposed populations.

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Section: Methodsmentioning

confidence: 99%

Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Büyükgüzel

Snela

et al. 2013

Cell Biol Toxicol

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“…Glutathione-S-transferase conjugates xenobiotics with reduced glutathione for excretion. The non-enzymatic components consist of small organic molecules such as reduced glutathione and vitamin C (Krishnan and Kodrík 2006; Krishnan et al 2009; Zhao and Shi 2009, 2010; Buyukguzel et al 2010). The tropical tasar silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae), produces silk of commercial importance.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Total Hydroperoxides and Antioxidant Defense System in the Indian Tropical Tasar Silkworm,Antheraea mylitta, in Diapausing and Non-Diapausing Generations

Jena

Kar

Babu

et al. 2013

Journal of Insect Science

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In the present study, the total hydroperoxides, catalase, glutathione-s-transferase, and ascorbic acid contents were determined in different developmental stages of the non-diapause and the diapause generation of the tropical tasar silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae). The results showed stage-specific significantly higher levels of total hydroperoxides, catalase, and ascorbic acid contents in the non-diapause as compared to the diapause generation (p < 0.05). However, a significantly enhanced level of glutathione-S-transferase activity was observed in mature 5th instar larvae of the diapause generation (p < 0.05). In the case of pupae, significantly higher levels of total hydroperoxides, catalase, and glutathione-s-transferase activity were observed in the non-diapause generation (p < 0.05). These results could be the effect of intensive metabolic transformation that takes place in tissues of the non-diapause generation and causes increased production of reactive oxygen species, such as hydroperoxides. The results suggest that antioxidants play an important role in protecting cells against reactive oxygen species.

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“…Hydroperoxides oxidize ferrous to ferric ions selectively in dilute acid and the resultant ferric and vitamin C (Felton and Summers,1995;Krishnan and Kodrik,2006;Krishnan et al, 2009;Buyukguzel et al, 2010;Shi 2009, 2010).…”

Section: Estimation Of Total Hydroperoxidesmentioning

confidence: 99%

Oxidative Stress and Antioxidant Defences in the Tasar Silkworm Antheraea mylitta D: Challenged with Nosema Species

Jena¹,

Pandey²,

Sinha³

2014

International Journal of Industrial Entomology

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This study was designed to find out the effect of Nosema spore on oxidative damages and antioxidant defence in the midgut of tasar silkworm Antheraea mylitta. Higher level of lipid peroxidation (LPX) and total hydroperoxides indicate the resultant oxidative stress in the Nosema exposed specimen. Increased superoxide dismutase (SOD) suggests activation of physiological mechanism to scavenge the superoxide radical produced during Nosema infection. Higher activities of catalase and glutathione-S-tranferase on 18 th d indicate adaptive behaviour of the tissue against oxyradicals. The results suggest that Nosema infection is involved in altering the active oxygen metabolism by modulating LPX and reactive oxygen species (ROS), which is indicative of pebrine disease disorder. As ingestion is the main entry route of many pathogens, the intestinal epithelium is the first line of defense against invasion and dissemination of pathogenic microorganisms.In this view insect innate immune system plays a vital role in the defense against microorganisms (Medzhitov and Janeway, 1997). One of the most immediate epithelial responses in mammals to combat the pathogen is the generation of antimicrobial reactive oxygen species (Cohn et al., 1994;Geiszt et al., 2003). It is to be noted that ROS can affect both entomopathogens and the host tissues, and may lead to damages of cellular biomolecules such as lipids, proteins and nucleic acids.To protect against these toxic cellular environment, insect possess a suit of antioxidant defence mechanisms, comprised of both enzymatic as well as non-enzymatic components.

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Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

Cited by 44 publications

References 60 publications

Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Comparative Study of Total Hydroperoxides and Antioxidant Defense System in the Indian Tropical Tasar Silkworm,Antheraea mylitta, in Diapausing and Non-Diapausing Generations

Oxidative Stress and Antioxidant Defences in the Tasar Silkworm Antheraea mylitta D: Challenged with Nosema Species

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