Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Büyükgüzel, Ender; Büyükgüzel, Kemal; Snela, Milena; Erdem, Meltem; Radtke, Katarzyna; Ziemnicki, Kazimierz; Adamski, Zbigniew

doi:10.1007/s10565-013-9240-7

Cited by 66 publications

(70 citation statements)

References 15 publications

(19 reference statements)

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“…Similar results were observed when Galleria mellonella (L., 1758) larvae were exposed to boric acid (Buyukguzel et al 2013) or when Tenebrio molitor (L., 1758) larvae were exposed to fenitrothion (Adamski et al 2005). We suggest that xenobiotics are transported through midgut cells relatively quickly.…”

Section: Disscussionsupporting

confidence: 57%

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Biological activity of Melia azedarach extracts against Spodoptera exigua

et al. 2014

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Melia azedarach is a plant species whose fruits posses nematicidal and insecticidal activity. In this study, we tested two M. azedarach extracts: a crude methanolic fruit extract (CRU) and its limonoids fraction (LIM) produced after partitioning with dichloromethane to check their insecticidal activity against beet armyworm Spodoptera exigua. Both extracts disturbed development of S. exigua. After exposure to CRU and LIM, hatching success was slightly lower, larvalpupal and pupal-imago moulting was significantly disturbed. In physiological studies, CRU extracts decreased the heartbeat of S. exigua pupae. Electron microscopic studies revealed that both extracts caused malformations of fat body and midgut cells. The most common observed malformations were: vacuolization of mitochondria, swollen endoplasmic reticulum and degeneration of nuclei. Obtained results indicated that M. azedarach fruits are a source of substances that may be used in plant protection against S. exigua. Despite the fact that M. azedarach extracts do not cause acute effects, their usage may lead to serious disturbances within exposed insects and significantly decrease activity of pests.

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Section: Disscussionsupporting

confidence: 57%

“…This tissue plays crucial role in detoxification and its malfunctioning may cause high toxicity to insects. Fusion of fat droplets was also observed when moths were exposed to other xenobiotics (Buyukguzel et al 2013). Fat body plays also important role in development.…”

Section: Disscussionmentioning

confidence: 90%

Biological activity of Melia azedarach extracts against Spodoptera exigua

et al. 2014

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“…In muscle tissue, interstitial Tables 1-3 and Figures 1-3. 4. Discussion BA is often used as a pesticide, and various non-target organisms are exposed to it (Büyükgüzel et al 2013). Sub-lethal concentrations of pesticides cause biochemical, histological and physiological changes in many tissues of aquatic organisms (Boran et al 2010).…”

Section: Experimental Groupsmentioning

confidence: 99%

“…BA is found naturally in water and seas at 1-5 ppm concentrations, and it may change the biological functions of different animal species. Therefore, it is frequently used as a pesticide, but high doses of BA and boron compounds are toxic to fish such as rainbow trout and zebrafish (Rowe et al 1998;Büyükgüzel et al 2013).…”

Section: Introductionmentioning

confidence: 99%

The effects of acute boric acid treatment on gill, kidney and muscle tissues in juvenile rainbow trout

Topal

Oruç

Altun

et al. 2015

Journal of Applied Animal Research

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Boric acid (BA) is an essential nutrient for plants and many organisms, but it has become an environmental contaminant because of widespread use. Pesticide and its compounds are a serious threat to aquatic organisms. This study was carried out to determine the histopathological effects of acute exposure to BA concentrations in rainbow trout. The fish were exposed to 10 2 and 10 3 mg/L concentrations of BA. Tissues were sampled at 6, 12, 24, 48 and 96 h. Histopathological alterations occurring in tissues were common in both doses of BA. Gill tissues showed lamellar oedema, cellulary infiltration, lamellar disorganization, degenerative changes and lamellar thickening. Kidneys had glomerular oedema and glomerulonephritis, degeneration of the tubulary epithelium, interstitial fibrosis and a hyaline cast within the tubular lumens. Muscle tissues displayed interstitial oedema and degenerative and atrophic changes to varying degrees in the myofibrils. Our study shows that BA can be toxic for rainbow trout and cause histopathological damage in fish tissue.

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“…Recently, insecticidal properties of BA on G. mellonella (Büyükgüzel et al, ; Hyršl et al, ), cockroaches (Cochran, ; Habes et al, ) and termites (Farid, Zaman, Saeed, Khan, & Bad Shah, ) have been reported. Studies reported that BA‐induced oxidative stress, altered protein profiles (Büyükgüzel et al, ; Hyršl et al, ) and also caused cuticular abrasion and destruction of the digestive tract wall (Cochran, ; Ebeling, ). These physiological effects could be related to oxidative stress and the suppression of the insect immune mechanisms leading to death.…”

Section: Discussionmentioning

confidence: 99%

Boric acid‐induced immunotoxicity and genotoxicity in model insect Galleria mellonella L. (Lepidoptera: Pyralidae)

Gwokyalya

Altuntaş

2019

Arch Insect Biochem Physiol

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Boric acid (BA) is widely used in various industrial process and can be accessed to nontarget organisms. This study aimed to investigate the insecticidal effects of BA and its toxic activities with respect to immunologic and genotoxic effects using Galleria mellonella larvae as a model. BA concentrations (78.125–10,000 ppm) were administrated to the larvae using the feeding method. Concentration‐dependent mortality was observed in all larval groups. Probit analysis revealed LC30, LC50, and LC70 values to be 112.4, 320.1, and 911.4 ppm, respectively. These concentrations were used in all bioassays. Drastic reductions in total hemocyte counts along with changes in differential hemocyte counts were observed following BA treatment. Cell viability assays showed dose‐dependent reductions in viable cells and an increase in the necrotic and apoptotic ratios after BA treatment. However, mitotic indices of larval hemocytes did not change at all BA concentrations. The cytotoxic effect of BA led to a significant reduction in cellular immune responses such as encapsulation, melanization, and nodulation activities of treated larvae. While BA increased micronucleus ratios at the highest concentration, comet parameters indicating DNA damage increased in G. mellonella larval hemocytes at all concentrations. These report that BA suppresses the immune system of G. mellonella and also poses risks of genotoxicity at high concentrations.

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Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Cited by 66 publications

References 15 publications

Biological activity of Melia azedarach extracts against Spodoptera exigua

Biological activity of Melia azedarach extracts against Spodoptera exigua

The effects of acute boric acid treatment on gill, kidney and muscle tissues in juvenile rainbow trout

Boric acid‐induced immunotoxicity and genotoxicity in model insect Galleria mellonella L. (Lepidoptera: Pyralidae)

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