Egr2 enhances insulin resistance via JAK2/STAT3/SOCS-1 pathway in HepG2 cells treated with palmitate

Lin, Li; Ye, Xinhua; Yao, Qing; Lu, Aijiao; Zhao, Zhen; Ding, Yang; Meng, Chuchen; Yu, Wenlong; Du, Yunfeng; Cheng, Jinquan

doi:10.1016/j.ygcen.2017.08.023

Cited by 25 publications

(19 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SOCS1 is a speci c negative regulator that regulates the JAK/STAT pathway [32]. The expression of SOCS1 increases under insulin resistance and overexpression of SOCS1 decreases the phosphorylation of IRS-1; overexpression of SOCS-1 can inhibit insulin-induced glycogen synthesis in L6 myotubes [33].…”

Section: Discussionmentioning

confidence: 99%

Resveratrol improves skeletal muscle insulin resistance through downregulating lncRNA NONMMUT044897.2

Liu

Zhang

Song

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Long non-coding RNA (lncRNA) has proved to be crucial factors in the progression of insulin resistance (IR). Resveratrol (RSV) exhibits promising therapeutic potential for the IR. Nonetheless, whether RSV could influence the expression of lncRNAs and the interaction mechanisms in IR remain unclear.Methods: We conducted high-throughput sequencing to detect the lncRNAs and mRNAs expression signatures and the co-expression network of lncRNAs and mRNAs in skeletal muscle after a high-fat diet (HFD)-induced IR mice model with or without RSV treatment, including hierarchical clustering, gene enrichment and gene co-expression networks analysis. Highly differentially expressed lncRNAs were selected and validated by RT-qPCR. Finally, the biological functions of the selected lncRNAs were investigated by silencing expressing the target genes through lentivirus transfection in C2C12 mouse myotubes cells.Results: We revealed that 338 mRNAs and 629 lncRNAs whose expression in skeletal muscle after a high-fat diet (HFD)-induced IR mice model was reversed by RSV treatment. Gene Ontology and Kyoto encyclopedia of genes and genomes databases indicated that the differential expression mRNAs modulate the insulin signaling pathway. After validating randomly selected lncRNAs via RT-qPCR, we found that lncRNA (NONMMUT044897.2) and Suppressor of Cytokine Signaling 1 (SOCS1) were up-regulated in the HFD group, and reversed by RSV treatment. Additionally, NONMMUT044897.2 was validated to function as a ceRNA of microRNA (miR)-7051-5p and SOCS1 was confirmed as a target for miR‑7051-5p. We further performed lentivirus transfection to knockdown NONMMUT044897.2 in vitro and found that NONMMUT044897.2 silence inactivated SOCS1 and promoted the insulin signaling pathway. Importantly, RSV could mimic the effects of silencing NONMMUT044897.2.Conclusion: Our study revealed that resveratrol improves skeletal muscle IR might be via regulation of NONMUT044897.2.

show abstract

Section: Discussionmentioning

confidence: 99%

Resveratrol improves skeletal muscle insulin resistance through downregulating lncRNA NONMMUT044897.2

Liu

Zhang

Song

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Chen et al found that the regulation of EGR2 expression by the competitive combination of LINC01939 with mir-17-5p may inhibit the metastasis and EMT of gastric cancer (Chen et al, 2019). EGR2 can reduce the phosphorylation of JAK2 and STAT3 by regulating the expression of SOCS-1 (Lu et al, 2017). In addition, a lack of EGR2 results in defective cloning amplification of T-cells as a response to viral infection, with overactivation and overdifferentiation (Miao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Expression and prognostic analyses of early growth response proteins (EGRs) in human breast carcinoma based on database analysis

Fei

Huang

et al. 2019

PeerJ

View full text Add to dashboard Cite

BackgroundEarly growth response proteins (EGRs), as a transcriptional regulatory family, are involved in the process of cell growth, differentiation, apoptosis, and even carcinogenesis. However, the role of EGRs in tumors, their expression levels, and their prognostic value remain unclear.MethodsUsing the Oncomine database, Kaplan–Meier Plotter, bcGenExMiner v4.2, cBioPortal, and other tools, the association between the survival data of breast carcinoma (BC) patients and transcriptional levels of four EGRs was investigated.ResultsAccording to the Oncomine database, in comparison to normal tissues, the expression level of EGR2/3 mRNA in BC tissues was decreased, but there was no difference in the expression level of EGR4 mRNA. On the basis of the Scarff-Bloom-Richardson (SBR) grading system, the downregulated expression level of EGR1/2/3 and upregulated expression level of EGR4 were correlated with an increased histological differentiation level, with significant differences (p < 0.05). Kaplan–Meier curves suggest that a reduction in EGR2/3 mRNA expression is related to recurrence-free survival (RFS) in BC patients. In addition, the mRNA expression level of EGR1/2/3 was related to metastatic relapse-free survival (MRFS) in BC patients with metastatic recurrence (p < 0.05).ConclusionEGR1/2/3 can be utilized as an important factor for evaluating prognosis and may be relevant to diagnosis. EGR4 may play a role in the occurrence and development of BC. The specific function and mechanism of EGRs in BC deserve further study.

show abstract

“…EGR plays a crucial role in the expression of FasL mediated by HBx, thus affecting the occurrence of HBV-related HCC[36]. Inhibition of EGR2 in HCC cell lines reduces the expression of SOCS-1 and the phosphorylation of JAK2 and STAT3, thus affecting cell proliferation[37]. FOS signal transduction is associated with TLR9-mediated IFN production in plasma-like dendritic cells, and the gene expression level of it is also significantly changed in HCC[38,39].…”

Section: Discussionmentioning

confidence: 99%

Identification of hepatitis B virus and liver cancer bridge molecules based on functional module network

Huang

Feng

et al. 2019

WJG

View full text Add to dashboard Cite

BACKGROUNDThe potential role of chronic inflammation in the development of cancer has been widely recognized. However, there has been little research fully and thoroughly exploring the molecular link between hepatitis B virus (HBV) and hepatocellular carcinoma (HCC).AIMTo elucidate the molecular links between HBV and HCC through analyzing the molecular processes of HBV-HCC using a multidimensional approach.METHODSFirst, maladjusted genes shared between HBV and HCC were identified by disease-related differentially expressed genes. Second, the protein-protein interaction network based on dysfunctional genes identified a series of dysfunctional modules and significant crosstalk between modules based on the hypergeometric test. In addition, key regulators were detected by pivot analysis. Finally, targeted drugs that have regulatory effects on diseases were predicted by modular methods and drug target information.RESULTSThe study found that 67 genes continued to increase in the HBV-HCC process. Moreover, 366 overlapping genes in the module network participated in multiple functional blocks. It could be presumed that these genes and their interactions play an important role in the relationship between inflammation and cancer. Correspondingly, significant crosstalk constructed a module level bridge for HBV-HCC molecular processes. On the other hand, a series of non-coding RNAs and transcription factors that have potential pivot regulatory effects on HBV and HCC were identified. Among them, some of the regulators also had persistent disorders in the process of HBV-HCC including microRNA-192, microRNA-215, and microRNA-874, and early growth response 2, FOS, and Kruppel-like factor 4. Therefore, the study concluded that these pivots are the key bridge molecules outside the module. Last but not least, a variety of drugs that may have some potential pharmacological or toxic side effects on HBV-induced HCC were predicted, but their mechanisms still need to be further explored.CONCLUSIONThe results suggest that the persistent inflammatory environment of HBV can be utilized as an important risk factor to induce the occurrence of HCC, which is supported by molecular evidence.

show abstract

Egr2 enhances insulin resistance via JAK2/STAT3/SOCS-1 pathway in HepG2 cells treated with palmitate

Cited by 25 publications

References 28 publications

Resveratrol improves skeletal muscle insulin resistance through downregulating lncRNA NONMMUT044897.2

Resveratrol improves skeletal muscle insulin resistance through downregulating lncRNA NONMMUT044897.2

Expression and prognostic analyses of early growth response proteins (EGRs) in human breast carcinoma based on database analysis

Identification of hepatitis B virus and liver cancer bridge molecules based on functional module network

Contact Info

Product

Resources

About