EGOFET Peptide Aptasensor for Label‐Free Detection of Inflammatory Cytokines in Complex Fluids

Berto, Marcello; Diacci, Chiara; D’Agata, Roberta; Pinti, Marcello; Bianchini, Elena; Lauro, Michele Di; Casalini, Stefano; Cossarizza, Andrea; Berggren, Magnus; Simon, Daniel T.; Spoto, Giuseppe; Biscarini, Fabio; Bortolotti, Carlo Augusto

doi:10.1002/adbi.201700072

Cited by 73 publications

(73 citation statements)

References 70 publications

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“…EGOTs (Figure 1.A), featuring a semiconductive channel exposed to an electrolyte whose potential is fixed by a Gate electrode, are ultra-sensitive sensors towards bio-markers, [17,18] ionic and molecular analytes, [19,20] and transducers of bioelectrical signals. [21,22] A prototypical bio-organic EGOT, light-gated by means of the photosynthetic RC from R. sphaeroides, is presented and termed Light-driven Electrolyte-Gated Organic Transistor -…”

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confidence: 99%

A Bacterial Photosynthetic Enzymatic Unit Modulating Organic Transistors with Light

Lauro

Gatta

Bortolotti

et al. 2019

Adv Elect Materials

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The photochemical core of every photosynthetic apparatus is the reaction center, a transmembrane enzyme that converts photons into charge-separated states across the biological membrane with an almost unitary quantum yield. We present a light-driven organic transistor architecture, which converts light into electrical current by exploiting the efficiency of this biological machinery. Proper surface tailoring enables the integration of the bacterial reaction center as photoactive element in organic transistors, allowing the transduction of its photogenerated voltage into photomodulation of the output current up to two orders of magnitude. The device architecture, termed Light-driven Electrolyte-Gated Organic Transistor (LEGOT), is the prototype of a new generation of low-power hybrid bio-optoelectronic organic devices. Main text Evolution has engineered multi-protein complexes to efficiently convert solar radiation into chemical energy, [1] sustaining the energy needs of life on planet Earth via the photosynthetic process. These photoenzymes catalyze the uphill conversion of oxidized molecules to their reduced forms, using light as energy source. Photosynthetic organisms, such as plants, algae, and some bacteria are the sole kind of organisms on the planet able to harvest and store energy. [2] The photosynthetic anoxygenic bacteria possess a photosynthetic apparatus based on a single functional unit, the reaction center (RC), which converts photons into charge-separated states across the membrane with unmatched quantum yield. Rhodobacter (R.) sphaeroides is a purple non-sulphur bacterium, whose RC is a three-subunit transmembrane protein sitting within the photosynthetic membrane. [3] Light impinges a cascade of electron transfer reactions that forms the hole-electron couple with a unitary quantum yield. [4] In absence of exogenous electron donors and acceptors, this state does not evolve further and has a lifetime ranging from hundred milliseconds up to three seconds. [5] See Figure S1 in the Supporting Information.

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confidence: 99%

A Bacterial Photosynthetic Enzymatic Unit Modulating Organic Transistors with Light

Lauro

Gatta

Bortolotti

et al. 2019

Adv Elect Materials

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“…Prospectively, the OECT complementary amplifier approach could be extended to a wide range of transistor-based bioelectronic technologies including for example, immunosensors 44 as well as metabolites 45 – 48 , hormones 49 , DNA 50 , peptides 51 , proteins 52 and viruses 53 , 54 , detection. Along this direction, an emerging and rapidly growing research field is the single molecule detection with millimeter-sized transistors 55 – 59 where the biorecognition events result in a shift of the transistor threshold voltage, which is typically of the order of few millivolts.…”

Section: Discussionmentioning

confidence: 99%

Multiscale real time and high sensitivity ion detection with complementary organic electrochemical transistors amplifier

et al. 2020

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Ions are ubiquitous biological regulators playing a key role for vital processes in animals and plants. The combined detection of ion concentration and real-time monitoring of small variations with respect to the resting conditions is a multiscale functionality providing important information on health states. This multiscale functionality is still an open challenge for current ion sensing approaches. Here we show multiscale real-time and high-sensitivity ion detection with complementary organic electrochemical transistors amplifiers. The ion-sensing amplifier integrates in the same device both selective ion-to-electron transduction and local signal amplification demonstrating a sensitivity larger than 2300 mV V −1 dec −1 , which overcomes the fundamental limit. It provides both ion detection over a range of five orders of magnitude and real-time monitoring of variations two orders of magnitude lower than the detected concentration, viz. multiscale ion detection. The approach is generally applicable to several transistor technologies and opens opportunities for multifunctional enhanced bioelectronics.

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“…Berto et al [52] first used His-Tag PG to immobilize the pro-inflammatory cytokine antibody for tumor necrosis factor alpha (TNF α) on the surface of a gold electrode, and demonstrated that gate voltage is the key factor for tuning the device sensitivity. In subsequent work, the authors realized the ultralow detection of TNF α in complex solutions such as a cell culture medium of 10% serum, which had obvious ligand specificity [53].…”

Section: Immobilization Strategies Of Aptamers and Other Moleculesmentioning

confidence: 99%

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

Wang

Xiao

et al. 2020

Trans. Tianjin Univ.

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Organic field-effect transistors (OFETs) are fabricated using organic semiconductors (OSCs) as the active layer in the form of thin films. Due to its advantages of high sensitivity, low cost, compact integration, flexibility, and printability, OFETs have been used extensively in the sensing area. For analysis platforms, the construction of sensing layers is a key element for their efficient detection capability. The strategy used to immobilize biomolecules in these devices is especially important for ensuring that the sensing functions of the OFET are effective. Generally, analysis platforms are developed by modifying the gate/electrolyte or OSC/electrolyte interface using biomolecules, such as enzymes, antibodies, or deoxyribonucleic acid (DNA) to ensure high selectivity. To provide better or more convenient biological immobilization methods for researchers in this field and thereby improve detection sensitivity, this review summarizes recent developments in the immobilization strategies used for biological macromolecules in OFETs, including cross-linking, physical adsorption, embedding, and chemical covalent binding. The influences of biomolecules on device performance are also discussed.

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EGOFET Peptide Aptasensor for Label‐Free Detection of Inflammatory Cytokines in Complex Fluids

Cited by 73 publications

References 70 publications

A Bacterial Photosynthetic Enzymatic Unit Modulating Organic Transistors with Light

A Bacterial Photosynthetic Enzymatic Unit Modulating Organic Transistors with Light

Multiscale real time and high sensitivity ion detection with complementary organic electrochemical transistors amplifier

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

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